200fr autoanalyser

Height and body mass were measured with the participants wearing light clothing and no shoes. BMI was calculated as body mass in kilograms divided by the square of the height in meters. WC (in cm) was measured mid-way between the costal margin and the iliac crest at the end of normal expiration. BP was measured on the right arm after resting for 5 min using an automatic manometer with an appropriate cuff size. After overnight fasting, early-morning blood samples were drawn from the antecubital vein into vacuum tubes and subsequently analysed by the central, certified laboratory at AMC. Measurements included concentrations of fasting glucose, insulin, hsCRP, several lipid parameters and liver enzymes.
Fasting total cholesterol, HDL-C, LDL-C, TG, uric acid, AST and ALT levels were measured using enzymatic colorimetric methods on a Toshiba 200FR Neo analyser (Toshiba Medical System Co., Ltd.). GGT was measured using the L-g-glutamyl-p-nitroanilide method (Toshiba Medical System Co., Ltd.). FPG and hsCRP were measured using the enzymatic colorimetric method on the Toshiba 200 FR auto-analyser and the immunoturbidimetric method (Toshiba Medical System Co., Ltd.), respectively. Ion-exchange high-performance liquid chromatography (Bio-Rad Laboratories, Inc., Hercules, CA, USA) was used to measure the HbA1c levels. All enzyme activities were measured at 37 °C.