Log In Sign up
The largest database of trusted experimental protocols

Anti ephrin a1

Manufactured by Santa Cruz Biotechnology
Visit Supplier

Anti-ephrin-A1 is a laboratory reagent used in research applications. It is an antibody that specifically binds to the ephrin-A1 protein, which is involved in cellular signaling pathways. The core function of Anti-ephrin-A1 is to facilitate the detection and study of ephrin-A1 in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Anti nestin, by Abcam (2 mentions) Anti ps374 akt, by Cell Signaling Technology (2 mentions) Mouse monoclonal anti p erk1 2, by Santa Cruz Biotechnology (2 mentions) Anti akt, by Cell Signaling Technology (2 mentions) Anti sox2, by R&D Systems (2 mentions) Anti gfap, by Merck Group (2 mentions) Anti epha2, by Santa Cruz Biotechnology (2 mentions) Anti erk1, by Santa Cruz Biotechnology (2 mentions) Anti gapdh, by Cell Signaling Technology (2 mentions) Epha2, by Cell Signaling Technology (1 mentions) S473 akt, by Cell Signaling Technology (1 mentions) Y397 fak, by Cell Signaling Technology (1 mentions) Gapdh, by Merck Group (1 mentions) Gsk3β, by Cell Signaling Technology (1 mentions)

3 protocols using anti ephrin a1

1

Protein Extraction and Antibody Detection

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were extracted and blotted as described in (27 (link)). Antibodies against S897-EphA2, Y772-EphA2, EphA2, Y397-FAK, FAK, Y118-PXN, PXN, S9-GSK3, GSK3β, S473-AKT, AKT, phospho-S/T AKT substrate and PTEN were purchased from Cell Signaling Technology (Danvers, MA). Anti-ephrinA1 was from Santa Cruz Biotechnology (Santa Cruz, CA) while GAPDH was purchased from Sigma Aldrich.
Paraiso K.H., Thakur M.D., Fang B., Koomen J.M., Fedorenko I.V., John J.K., Tsao H., Flaherty K.T., Sondak V.K., Messina J.L., Pasquale E.B., Villagra A., Rao U.N., Kirkwood J.M., Meier F., Sloot S., Gibney G.T., Stuart D., Tawbi H, & Smalley K.S. (2014). Ligand independent EphA2 signaling drives the adoption of a targeted therapy-mediated metastatic melanoma phenotype. Cancer discovery, 5(3), 264-273.
+ Open protocol
+ Expand
2

Characterization of Antibodies for EphA2 Signaling

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Rabbit polyclonal anti-pS374-Akt, anti-Akt, and anti-GAPDH were purchased from Cell Signaling. Rabbit anti-p-EphA/B was raised against the conserved phosphopeptides from the juxtamembrane regions. Rabbit polyclonal phospho-S897-specific antibody was raised against phospho-peptide DPRVSIRLP-pS897-TSGSEGVPFR and has been characterized previously (19 (link)). Rabbit polyclonal anti-EphA2, anti-ephrin-A1, anti-ERK1, mouse monoclonal anti-p-ERK1/2 were purchased from Santa Cruz Biotechnology. Mouse monoclonal anti-EphA2 was a kind gift from Dr. Andrew Boyd (The University of Queensland, Australia). Mouse monoclonal anti-Sox2 was purchased from R&D System. Rabbit polyclonal anti-Nestin were purchased from Abcam. Mouse monoclonal anti-GFAP was purchased from Sigma. Ephrin-A1-Fc was produced as described (49 (link)).
Miao H., Gale N.W., Guo H., Qian J., Petty A., Kaspar J., Murphy A.J., Valenzuela D.M., Yancopoulos G., Hambardzumyan D., Lathia J.D., Rich J.N., Lee J, & Wang B. (2014). EphA2 Promotes Infiltrative Invasion of Glioma Stem Cells in vivo through Crosstalk with Akt and Regulates Stem Properties. Oncogene, 34(5), 558-567.
+ Open protocol
+ Expand
3

Characterization of Antibodies for EphA2 Signaling

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Rabbit polyclonal anti-pS374-Akt, anti-Akt, and anti-GAPDH were purchased from Cell Signaling. Rabbit anti-p-EphA/B was raised against the conserved phosphopeptides from the juxtamembrane regions. Rabbit polyclonal phospho-S897-specific antibody was raised against phospho-peptide DPRVSIRLP-pS897-TSGSEGVPFR and has been characterized previously (19 (link)). Rabbit polyclonal anti-EphA2, anti-ephrin-A1, anti-ERK1, mouse monoclonal anti-p-ERK1/2 were purchased from Santa Cruz Biotechnology. Mouse monoclonal anti-EphA2 was a kind gift from Dr. Andrew Boyd (The University of Queensland, Australia). Mouse monoclonal anti-Sox2 was purchased from R&D System. Rabbit polyclonal anti-Nestin were purchased from Abcam. Mouse monoclonal anti-GFAP was purchased from Sigma. Ephrin-A1-Fc was produced as described (49 (link)).
Miao H., Gale N.W., Guo H., Qian J., Petty A., Kaspar J., Murphy A.J., Valenzuela D.M., Yancopoulos G., Hambardzumyan D., Lathia J.D., Rich J.N., Lee J, & Wang B. (2014). EphA2 Promotes Infiltrative Invasion of Glioma Stem Cells in vivo through Crosstalk with Akt and Regulates Stem Properties. Oncogene, 34(5), 558-567.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!