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Cary 5000 series uv vis nir spectrophotometer

Manufactured by Agilent Technologies

The Cary 5000 Series UV-Vis-NIR Spectrophotometer is a high-performance laboratory instrument designed for the measurement of absorbance, transmittance, and reflectance across the ultraviolet, visible, and near-infrared wavelength ranges. It provides accurate and reliable data for a wide range of applications in materials science, chemistry, and life sciences.

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4 protocols using cary 5000 series uv vis nir spectrophotometer

1

Photoisomerization of 3-(4-Phenylazophenoxy)propanol

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Photoisomerization measurements were performed on 3-(4-phenylazophenoxy)propanol using a previously established procedure.12 (link) A sample of 1 mg mL−1 UCNPs and 4.3 × 10−6 M 3-(4-phenylazophenoxy)propanol in hexanes, was irradiated at 30 min increments using a 976 nm laser (Coherent 6-pin 15 fiber-coupled F6 series laser diode operating at a power density of 42.9 W cm−2, controlled using an ILX Lightwave LDC-3744B). The recording of a UV-Vis absorption spectrum was obtained using a Cary 5000 Series UV-Vis-NIR Spectrophotometer (Agilent Technologies), operating with a scan speed of 600 nm s−1 and a resolution of 1 nm.
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2

Characterization of Organic Compounds

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All UV-visible absorption spectra were collected on a Cary 5000 series UV-vis-NIR spectrophotometer from Agilent Technologies. Solutions were analyzed in a 1 cm path length quartz cuvette from Thorlabs. All 1H-NMR spectra were recorded on a 500 MHz Varian Scientific NMR spectrometer at 25 °C in DMSO-d6.
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3

UV-Vis Characterization of Aqueous CD

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UV-Vis absorbance spectra of the aqueous CD dispersions were collected using a Cary 5000 Series UV-Vis-NIR Spectrophotometer from Agilent Technologies. The scans were acquired over a range of 200–800 nm in a 1-cm quartz cuvette at a speed of 600 nm/s, 1 nm resolution and a 2-nm bandwidth. A Peltier Temperature Controller from Agilent Technologies was used to heat and cool the sample cell for temperature experiments. Data collection and processing were performed using the Agilent Cary Eclipse Scan software package.
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4

Air-Sensitive Iron Complex Synthesis

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Unless otherwise specified, all air sensitive manipulations were carried out either in a N2 filled glove box or using standard Schlenk techniques under Ar. Proligand L2 was prepared according to a literature procedure. [23] FeCl2 (Acros), NaPF6 (Alfa Aesar), NaBPh4 (Sigma-Aldrich), and nBu4NPF6 (electrochemical grade, Sigma-Aldrich) were purchased and used without further purification. Organic solvents were dried and distilled using appropriate drying agents prior to use. 1and 2D NMR spectra were recorded on Bruker Avance 300 MHz or Bruker Avance-III 500 MHz spectrometers. 1 H and 13 C{ 1 H} NMR spectra were referenced to residual solvent peaks. Electronic absorption spectra were recorded on an Agilent Technologies Cary 5000 Series UV-Vis-NIR spectrophotometer in dual beam mode (range: 230 -1600 nm). Mössbauer experiments were performed in transmission geometry with a 57 Co in Rh source and a WissEl constant acceleration drive. Spectra were collected at 10 K using a Janis SHI-850 closed-cycle refrigerator and are calibrated relative to a-Fe at room temperature.
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