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Sc-30210 is a highly specific monoclonal antibody produced by the Developmental Studies Hybridoma Bank. It is designed for research purposes and can be used in various immunological techniques to detect and analyze specific target proteins.

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Lab products found in correlation

Ab52903, by Abcam (1 mentions) Rat anti vasa, by Developmental Studies Hybridoma Bank (1 mentions)

2 protocols using sc 30210

1

Immunostaining Techniques for Drosophila Proteins

Cited 1 time
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For immunostaining, the following primary antibodies were used: rabbit anti-CENP-A (CID) antibody (active motif 39719; 1:500), sheep anti-CENP-C (Dattoli et al., 2020 (link)); 1:2000), rabbit anti-VASA (Santa Cruz sc-30210; 1:500), rat anti-VASA (Developmental Studies Hybridoma Bank [DSHB]; 1:500), sheep anti-CAL1 (this study; 1:1000), rabbit anti-SMAD3/5 (pMad; Abcam ab52903; 1:500), DAPI (1:1000), mouse anti-Armadillo (DSHB N2 7A1; 1:700), mouse anti-1B1 (Hts; DSHB; 1:100), mouse anti-BAM (DSHB; 1:50), mouse anti-FIBRILLARIN (Abcam; ab5821; 1:500), mouse anti-HA (ThermoFisher; 26183; 1:500), mouse anti-gamma-tubulin (Abcam; ab44928; 1:500), mouse anti-FASCILLIN III (DSHB 7G10; 1:200), rat anti-mCherry (Chromotek 5F8; 1:200). Sheep anti-CAL1 antibody was raised against CAL1 amino acids 748–979 with a C-terminal His tag produced according to Bade et al. (2014 (link)). Species-specific Alexa Fluor 405, 488, 546, and 647 secondary antibodies were used at a 1:500 dilution.
Kochendoerfer A.M., Keegan R.S, & Dunleavy E.M. (2023). Centromere proteins are asymmetrically distributed between newly divided germline stem and daughter cells and maintain a balanced niche in Drosophila males. Molecular Biology of the Cell, 34(5), ar42.
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2

Immunostaining Assay for Drosophila Mitotic Proteins

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For immunostaining, the following antibodies were used: rabbit anti-CENP-A (CID) antibody (Active Motif 39719; 1:1000), rat anti-CID antibody (Active Motif 61735, 1:500), sheep anti-CENP-C (Dattoli et al, 2020; 1:2000), mouse anti-H3S10P (Abcam ab14955; 1:1,000), rabbit anti-VASA (Santa Cruz sc-30210; 1:300), rat anti-VASA (Developmental Studies Hybridoma Bank (DSHB); 1.500), mouse anti-Hts (1B1, DSHB; 1:500), rabbit anti-CAL1 (Bade et al, 2014; 1:1000), rabbit anti-SMAD3/5 (pMad) (Abcam; 1:500), mouse anti-SEX-LETHAL (DSHB, M114, 1:500), sheep anti-Spc105 (M. Przewloka; 1:2000), DAPI (1:1000), Hoechst (1:1000).
Carty B.L., Dattoli A.A, & Dunleavy E.M. (2021). CENP-C functions in centromere assembly, the maintenance of CENP-A asymmetry and epigenetic age in Drosophila germline stem cells. PLoS Genetics, 17(5), e1009247.
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