All non-cancer cell lines used were human and were cultured at 37°C under 5% CO2 and 20% oxygen. THLE3 (normal liver) cells were a kind gift of the Kwan Yong Choi Lab (POSTECH, Korea). CCD841CoN (normal colon), CCD18Lu (normal lung), HS67 (normal thymus), HIEC6 (normal small intestine) were purchased from ATCC, and GM05565 and GM02037 (normal skin fibroblasts) were purchased from the Corriel Institute. THLE3 cells were cultured in BEBM Bronchial Epithelial Cell Growth Basal Medium (Lonza Cat#CC-3171) supplemented with the BEGM SingleQuots (Lonza Cat#CC-4175), 5ng/mL EGF, 70ng/mL Phosphoethanolamine, and 10% FBS. CCD841CoN, CCD18Lu, GM05565, and GM02037 were cultured in Eagle’s Minimum Essential Medium (ATCC Cat#30–2003) with 10% FBS (Sigma #F2442) and 100 units/ml Penicillin-Streptomycin. HS67 were cultured in Dulbecco’s Modified Eagle Medium (DMEM, high glucose, pyruvate) supplemented with 10% FBS, 100 units/ml Penicillin-Streptomycin, and 2mM L-Glutamine. HIEC6 were cultured in OptiMEM 1 Reduced Serum Medium (Gibco Cat#31985), 20 mM HEPES, 10 mM GlutaMAX (Gibco Cat#35050061), 10ng/ml EGF, 4% FBS, and 100 units/ml Penicillin-Streptomycin.
Bebm bronchial epithelial cell growth basal medium
Manufactured by Lonza
Sourced in Sweden, Switzerland
BEBM Bronchial Epithelial Cell Growth Basal Medium is a cell culture medium formulated to support the growth and maintenance of human bronchial epithelial cells in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Ccd 841 con, by American Type Culture Collection (2 mentions)
Opti mem 1 reduced serum medium, by Thermo Fisher Scientific (2 mentions)
Ccd 18lu, by American Type Culture Collection (2 mentions)
Gm05565, by American Type Culture Collection (2 mentions)
Gm02037, by American Type Culture Collection (2 mentions)
Hiec6, by Thermo Fisher Scientific (2 mentions)
Hiec 6, by American Type Culture Collection (2 mentions)
Glutamax, by Thermo Fisher Scientific (2 mentions)
Begm singlequots, by Lonza (2 mentions)
Cc 4175, by Lonza (1 mentions)
Begm bronchial epithelial cell growth medium, by Lonza (1 mentions)
Gentamicin amphotericin b, by Lonza (1 mentions)
Human multiplex cytokine assay, by Eve Technologies (1 mentions)
Milli q, by Merck Group (1 mentions)
Bronchial epithelial singlequots kit, by Lonza (1 mentions)
Formic acid, by Merck Group (1 mentions)
Nystatin, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Retinoic acid, by Merck Group (1 mentions)
6 protocols using bebm bronchial epithelial cell growth basal medium
1
Cell Line Culture Optimization and Maintenance
2
Culturing Normal Human Bronchial Epithelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary normal human bronchial epithelial (NHBE) cells were obtained from LONZA Walkersville Inc. (NHBE CC-2540; Lonza) from a single anonymous female donor, who was a non-smoker with no respiratory pathology. NHBE cells were seeded and grown according to the manufacturer's instructions. Briefly, cells were passaged once into a T25 flask in BEBM Bronchial Epithelial Cell Growth Basal Medium (CC-3171, Lonza) with BEGM Bronchial Epithelial Cell Growth Medium SingleQuots Supplements and Growth Factors, containing Bovine Pituitary Extract [BPE], Hydrocortisone, human Epidermal Growth Factor [hEGF], Epinephrine, Transferrin, Insulin, Retinoic Acid, Triiodothyronine, and Gentamicin/Amphotericin-B (CC-4175, Lonza). The growth media was changed every 48–72 h. When cells exceeded 45% confluence, the volume of the medium was doubled. Once cells reach 75–85% confluence, cells were re-seeded at 100,000 cells/T25 flask. Cells were passaged every seven days or when 85% confluency was reached. We used Clonetics ReagentPack (CC-5034, Lonza) for cell subculture with HEPES Buffered Saline Solution, Trypsin/EDTA, and Trypsin Neutralizing Solution. Cells were maintained at 37 °C in a 5% CO2 atmosphere. All experiments were performed between passages 1–5.
3
Cell Line Culture Optimization and Maintenance
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All non-cancer cell lines used were human and were cultured at 37°C under 5% CO2 and 20% oxygen. THLE3 (normal liver) cells were a kind gift of the Kwan Yong Choi Lab (POSTECH, Korea). CCD841CoN (normal colon), CCD18Lu (normal lung), HS67 (normal thymus), HIEC6 (normal small intestine) were purchased from ATCC, and GM05565 and GM02037 (normal skin fibroblasts) were purchased from the Corriel Institute. THLE3 cells were cultured in BEBM Bronchial Epithelial Cell Growth Basal Medium (Lonza Cat#CC-3171) supplemented with the BEGM SingleQuots (Lonza Cat#CC-4175), 5ng/mL EGF, 70ng/mL Phosphoethanolamine, and 10% FBS. CCD841CoN, CCD18Lu, GM05565, and GM02037 were cultured in Eagle’s Minimum Essential Medium (ATCC Cat#30–2003) with 10% FBS (Sigma #F2442) and 100 units/ml Penicillin-Streptomycin. HS67 were cultured in Dulbecco’s Modified Eagle Medium (DMEM, high glucose, pyruvate) supplemented with 10% FBS, 100 units/ml Penicillin-Streptomycin, and 2mM L-Glutamine. HIEC6 were cultured in OptiMEM 1 Reduced Serum Medium (Gibco Cat#31985), 20 mM HEPES, 10 mM GlutaMAX (Gibco Cat#35050061), 10ng/ml EGF, 4% FBS, and 100 units/ml Penicillin-Streptomycin.
4
Cytokine Profiling in Airway Epithelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Non‐transfected and transfected BEAS‐2B cells were cultured in 12‐well plates and grown to ~80% confluency. Then, cells were starved using BEBM™ Bronchial Epithelial Cell Growth Basal Medium (Lonza Inc.) and the medium was collected 24 h later. Samples were centrifuged at 800 g for 5 mins at 4°C and stored at −80°C until needed. The concentration of human TNFα, IL‐1β, IL‐4, IL‐5, IL‐6, IL‐8, IL‐10, IL‐13, CCL2, GM‐CSF, and IFNγ were measured using a commercially available human multiplex cytokine assay (Eve Technologies).
5
Nano-DESI solvent composition and cell culture medium
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the nano-DESI solvent, HPLC-MS grade methanol (BDH Chromanorm Solvents, VWR International AB, Stockholm, Sweden), deionized water (18.2 MΩ, Milli-Q, Millipore, Solna, Sweden), and formic acid (98-100%, Merck, Darmstadt, Germany) were used. Interleukine-13 and histamine were purchased (Sigma-Aldrich, Stockholm, Sweden), and budesonide and terbutaline were extracted from commercially available products. The medium used for growing the cells consisted of 50 mL of Dulbecco’s Modified Eagle Medium (500 mL of D-MDM, 5 mL of 100 mM Minimum Essential Medium Sodium pyruvate (Gibco, MEM NaPyr, Fisher Scientific, Gothenburg, Sweden), 5 mL of 200 mM L-Glutamine, 5 mL of 100× Minimum Essential Medium Non-Essential Amino Acids (Gibco MEM NEA, Fisher Scientific, Gothenburg, Sweden), and 50 mL ALI medium (250 mL of Bronchial Epithelial Cell Growth Basal Medium (BEBM, Lonza Bioscience, Stockholm, Sweden), 1 vial of Bronchial Epithelial SingleQuots Kit (BEGM, Lonza Bioscience, Stockholm, Sweden), and 500 µL 1.5 mg/mL BSA), with an addition of 50 µL 0.1 mM retinoic acid. All the other reagents were purchased from Thermo Fisher, Waltham, MA, USA.
6
Culturing Primary Human Bronchial Epithelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary HBE cells were obtained from the Cystic Fibrosis Center Tissue Procurement and Cell Culture Core, under the approved Protocol (No. 03–1396) by the Institutional Review Board at the University of North Carolina at Chapel Hill. We denoted non-asthmatic cells for the cells isolated from eight donors with no history of smoking or chronic lung disease and asthmatic cells for the cells isolated from three donors with fatal asthma and one donor with non-fatal asthma (Table S1 ). As described previously [12 (link),13 (link),47 (link),48 (link),49 (link),50 (link)], passage 2 of primary HBE cells were cultured and maintained in air–liquid interface (ALI). For the ALI culture, we used a 1:1 mixture of Dulbecco’s modified Eagle’s medium (DMEM, Lonza, Basel, Switzerland) and Bronchial epithelial cell growth basal medium (BEBM, Lonza) supplemented with Bronchial epithelial growth medium (BEGM) SingleQuot kit supplement and growth factors (Lonza), nystatin (20 units/mL, Sigma Aldrich, St. Louis, MO, USA), retinoic acid (50 nM, Sigma Aldrich), and bovine serum albumin (1.5 µg/mL, Sigma Aldrich). In this study, we used the HBE cells on ALI days 14–16, where we consistently observed well-differentiated epithelial cell phenotypes [11 (link),16 (link),51 (link),52 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!