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Anti cd45.2 apc cy7 clone 104

Manufactured by BioLegend
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Anti-CD45.2-APC-Cy7 is a fluorochrome-conjugated monoclonal antibody that binds to the CD45.2 antigen. CD45 is a protein tyrosine phosphatase expressed on the surface of most hematopoietic cells.

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Lab products found in correlation

Lsrfortessa cell analyzer, by BD (2 mentions) Anti mhcii bv421 clone m5 114.15.2, by BD (2 mentions) Anti cd21 phycoerythrin pe cf594 clone 7g6, by BD (2 mentions) Anti cd23 allophycocyanine apc clone b3b4, by BioLegend (2 mentions) Anti cd45.1 pe clone a20, by BD (2 mentions) Anti cd86 bv510 clone gl1, by BD (2 mentions) Anti cd21 pe cf594 clone 7g6, by BD (2 mentions) Anti cd23 pe cy7 clone b3b4, by BD (2 mentions) Anti mhcii fluorescein isothiocyanate fitc clone 2g9, by BD (2 mentions) Anti pstat1 af647 clone 4a, by BD (2 mentions) Anti cd62l apc, by BioLegend (1 mentions) Anti il 2 pe, by BioLegend (1 mentions) Anti ifn γ fitc, by BioLegend (1 mentions) Anti mhc 2 fitc, by BioLegend (1 mentions) Anti cd8 percp cy5.5 clone 53 6 7, by BioLegend (1 mentions) Anti cd127 pe, by BioLegend (1 mentions) Anti tnf apc, by BioLegend (1 mentions)

Close spelling variants of this product

Anti-CD45 (228 citations) CD45-APC-Cy7 (107 citations) APC-Cy7 (106 citations) CD45-APC (97 citations) Anti-CD45-APC-Cy7 (45 citations) Anti-CD45-APC (42 citations) CD45.2 (clone 104, (29 citations) CD45.2 (104, (22 citations) Anti-CD45.2 (104, (17 citations) Anti-CD45.2 (clone 104, (13 citations)

3 protocols using anti cd45.2 apc cy7 clone 104

1

Multiparameter Flow Cytometry Protocol

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The following Abs were used from Biolegend (San Diego, California): anti-CD8 PerCP/Cy5.5 (clone 53-6.7), anti-CD4 PerCP/Cy5.5 (clone RM4-5), anti-CD25 PE (clone PC61.5), anti-CD69 FITC (clone H1.2F3), anti-CD40 PE (clone 1C10), anti-CD80 PE (clone 16-10A1), anti-CD86 PE (clone GL-1), anti-Thy1.1 APC, (clone OX-7), anti-CD45.2 APC/Cy7 (clone 104), anti-CD19 PerCP/Cy5.5 (clone 6D5), anti-CD11c APC (clone N418), anti-B220 PE (clone RA3-6B2), anti-MHC II FITC (clone M5/114.15.2), anti-CD62L APC (clone MEL-14), anti-CD127 PE (clone A7R34), anti-KLRG1 APC (clone 2F1/KLRG1), anti-IFN-γ FITC (clone XMG1.2), anti-TNF APC (clone MP6-XT22), and anti-IL-2 PE (clone JES6-5H4). The following antibody was purchased from eBiosciences (Dan Diego, CA): anti-CD27 PE (clone LG.7F9). The following antibody was purchased from Caltag Laboratories anti-granzyme B-PE.
White C.E., Villarino N.F., Sloan S.S., Ganusov V.V, & Schmidt N.W. (2014). Plasmodium suppresses expansion of T cell responses to heterologous infections. Journal of immunology (Baltimore, Md. : 1950), 194(2), 697-708.
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2

Flow Cytometric Analysis of IFN-Signaling in B Cells

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The presence of PKH67-stained LV9 on various B cell subsets was assessed using the following antibodies: anti-CD21-phycoerythrin (PE)-CF594 (clone 7G6; BD Biosciences), anti-CD23-allophycocyanine (APC) (clone B3B4; BioLegend), anti-CD45.1-PE (clone A20; BD Biosciences), anti-CD45.2-APC-Cy7 (clone 104; BioLegend), anti-CD86-BV510 (clone GL1; BD Biosciences), and anti-MHCII-BV421 (clone M5/114.15.2; BD Biosciences). To conserve the phosphorylation status during the measurement of type I IFN signaling, cells were fixed in 2% PFA prior to permeabilization using cold methanol and staining using the following antibody panel: anti-CD21-PE-CF594 (clone 7G6; BD Biosciences), anti-CD23-PE-Cy7 (clone B3B4; BD Biosciences), anti-MHCII-fluorescein isothiocyanate (FITC) (clone 2G9; BD Biosciences), and anti-pSTAT1-AF647 (clone 4a; BD Biosciences). For each sample, 100,000 cells were acquired using an LSRFortessa cell analyzer (BD), and data were analyzed using FlowJo software (BD).
Stögerer T., Silva-Barrios S., Carmona-Pérez L., Swaminathan S., Mai L.T., Leroux L.P., Jaramillo M., Descoteaux A, & Stäger S. (2023). Leishmania donovani Exploits Tunneling Nanotubes for Dissemination and Propagation of B Cell Activation. Microbiology Spectrum, 11(4), e05096-22.
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3

Flow Cytometric Analysis of IFN-Signaling in B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The presence of PKH67-stained LV9 on various B cell subsets was assessed using the following antibodies: anti-CD21-phycoerythrin (PE)-CF594 (clone 7G6; BD Biosciences), anti-CD23-allophycocyanine (APC) (clone B3B4; BioLegend), anti-CD45.1-PE (clone A20; BD Biosciences), anti-CD45.2-APC-Cy7 (clone 104; BioLegend), anti-CD86-BV510 (clone GL1; BD Biosciences), and anti-MHCII-BV421 (clone M5/114.15.2; BD Biosciences). To conserve the phosphorylation status during the measurement of type I IFN signaling, cells were fixed in 2% PFA prior to permeabilization using cold methanol and staining using the following antibody panel: anti-CD21-PE-CF594 (clone 7G6; BD Biosciences), anti-CD23-PE-Cy7 (clone B3B4; BD Biosciences), anti-MHCII-fluorescein isothiocyanate (FITC) (clone 2G9; BD Biosciences), and anti-pSTAT1-AF647 (clone 4a; BD Biosciences). For each sample, 100,000 cells were acquired using an LSRFortessa cell analyzer (BD), and data were analyzed using FlowJo software (BD).
Stögerer T., Silva-Barrios S., Carmona-Pérez L., Swaminathan S., Mai L.T., Leroux L.P., Jaramillo M., Descoteaux A, & Stäger S. (2023). Leishmania donovani Exploits Tunneling Nanotubes for Dissemination and Propagation of B Cell Activation. Microbiology Spectrum, 11(4), e05096-22.
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