In this study, we used bone, cartilage and fat differentiation test to identify the nature of the isolated cells. The confluent cells were cultured in an osteogenic (DMEM including 50 µg/mL ascorbic acid 3-phosphate (Sigma Chemical Co. St Louis, MO, USA), 10 nM dexamethasone (Sigma Chemical Co.), 10 mM β-glycerol phosphate (Sigma Chemical Co.), and adipogenic (DMEM supplemented with 50 µg/mL ascorbic acid 2-phosphate (Sigma Chemical Co), 100 nM dexamethasone (Sigma Chemical Co.), 50 µg/mL indomethacin (Sigma Chemical Co), chondrogenic (DMEM supplemented with 50 µg/mL ascorbic acid 2-phosphate (Sigma Chemical Co), 10 nM dexamethasone (Sigma Chemical Co.), transforming growth factor-ß3 (TGF-ß3; Sigma Chemical Co), bone morphogenetic protein- 6 (BMP-6), and insulin– transferrin– selenium (ITS; GIBCO- BRL) medium. At the end of the differentiation period, the cells were evaluated byalizarin red staining for osteoblasts,alcian blue staining for chondroblasts andoil red staining for adipocytes.
Tgf 3
Manufactured by Merck Group
Sourced in United States
TGF-ß3 is a protein that belongs to the transforming growth factor beta (TGF-β) family. It is a laboratory product primarily used for research purposes in cell biology and molecular biology studies.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Ascorbic acid 3 phosphate, by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Indomethacin, by Merck Group (1 mentions)
Ascorbic acid 2 phosphate, by Merck Group (1 mentions)
β glycerol phosphate, by Merck Group (1 mentions)
Proline, by Merck Group (1 mentions)
Bmp 6, by Merck Group (1 mentions)
Igf 1, by Merck Group (1 mentions)
Sodium pyruvate, by Merck Group (1 mentions)
Ascorbate 2 phosphate, by Merck Group (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
L ascorbic acid 2 phosphate, by Merck Group (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Amphotericin b, by Thermo Fisher Scientific (1 mentions)
3 protocols using tgf 3
1
Multilineage Differentiation Potential
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In this study, we used bone, cartilage and fat differentiation test to identify the nature of the isolated cells. The confluent cells were cultured in an osteogenic (DMEM including 50 µg/mL ascorbic acid 3-phosphate (Sigma Chemical Co. St Louis, MO, USA), 10 nM dexamethasone (Sigma Chemical Co.), 10 mM β-glycerol phosphate (Sigma Chemical Co.), and adipogenic (DMEM supplemented with 50 µg/mL ascorbic acid 2-phosphate (Sigma Chemical Co), 100 nM dexamethasone (Sigma Chemical Co.), 50 µg/mL indomethacin (Sigma Chemical Co), chondrogenic (DMEM supplemented with 50 µg/mL ascorbic acid 2-phosphate (Sigma Chemical Co), 10 nM dexamethasone (Sigma Chemical Co.), transforming growth factor-ß3 (TGF-ß3; Sigma Chemical Co), bone morphogenetic protein- 6 (BMP-6), and insulin– transferrin– selenium (ITS; GIBCO- BRL) medium. At the end of the differentiation period, the cells were evaluated byalizarin red staining for osteoblasts,alcian blue staining for chondroblasts andoil red staining for adipocytes.
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In this study, we used bone, cartilage and fat differentiation test to identify the nature of the isolated cells. The confluent cells were cultured in an osteogenic (DMEM including 50 µg/mL ascorbic acid 3-phosphate (Sigma Chemical Co. St Louis, MO, USA), 10 nM dexamethasone (Sigma Chemical Co.), 10 mM β-glycerol phosphate (Sigma Chemical Co.), and adipogenic (DMEM supplemented with 50 µg/mL ascorbic acid 2-phosphate (Sigma Chemical Co), 100 nM dexamethasone (Sigma Chemical Co.), 50 µg/mL indomethacin (Sigma Chemical Co), chondrogenic (DMEM supplemented with 50 µg/mL ascorbic acid 2-phosphate (Sigma Chemical Co), 10 nM dexamethasone (Sigma Chemical Co.), transforming growth factor-ß3 (TGF-ß3; Sigma Chemical Co), bone morphogenetic protein- 6 (BMP-6), and insulin– transferrin– selenium (ITS; GIBCO- BRL) medium. At the end of the differentiation period, the cells were evaluated byalizarin red staining for osteoblasts,alcian blue staining for chondroblasts andoil red staining for adipocytes.
2
Chondrogenic Differentiation of Cell-Seeded PU-Fibrin Constructs
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After polymerization, chondrogenic differentiation medium, which consisted of DMEM supplemented with 1% P/S, 100 nM dexamethasone, 100 µg/mL sodium pyruvate (Sigma-Aldrich), 50 µg/mL ascorbate-2-phosphate (Sigma-Aldrich), 40 µg/mL proline (Sigma-Aldrich), and 1% ITS-plus liquid media supplement (Sigma-Aldrich), was added to the cell-seeded PU-fibrin constructs. Expansion medium (EM-DMEM) was used for the control group:
Group (DMEM): Control group maintained in expansion medium.
Group (BT): 50 ng/mL TGF-ß3 and 500 ng/mL BMP-6 (PromoCell GmbH, Heidelberg, Germany).
Group (BBT): 500 ng/mL BMP-6 for 7 days, followed by 50 ng/mL TGF-ß3 and 500 ng/mL BMP-6.
Group (TI): 50 ng/mL TGF-ß3 and 100 ng/mL IGF-1 (Sigma-Aldrich).
3
Corneal Stromal Stem Cell Differentiation
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cCSSCs at the third passage were seeded into SF/G stromal scaffold at a concentration of 6x106 cells/mL; 80 μl for construction of the corneal stromal part and seeded into the culture plate for characterization. After 3 days, the media were changed from corneal stromal stem cell proliferation media to keratocyte differentiation media (KDM), consisting of DMEM (Thermo Fisher Scientific Corporation) supplemented with 1.0 mM L ascorbic acid-2-phosphate (Sigma-Aldrich Corporation, St Louis, USA), 2 mM L-glutamine (100x GlutaMAX™; Thermo Fisher Scientific Corporation), 100 unit/mL penicillin (Thermo Fisher Scientific Corporation), 100 μg/mL streptomycin (Thermo Fisher Scientific Corporation), 5 μg/mL amphotericin B (Thermo Fisher Scientific Corporation), 10 ng/mL basic fibroblast growth factor (FGF-2, Millipore corporation), and 0.1 ng/mL transforming growth factor-beta3 (TGF-ß3, Sigma-Aldrich Corporation, USA).
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