Igf 1

Manufactured by BD

Sourced in United States

IGF-1 is a laboratory assay used to measure the levels of insulin-like growth factor 1 (IGF-1) in biological samples. IGF-1 is a hormone that plays a crucial role in growth and development. The IGF-1 assay provides quantitative information about the concentration of this hormone, which can be used to assess various physiological and clinical conditions.

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Lab products found in correlation

Neurocult ns a differentiation kit, by STEMCELL (1 mentions) T 25 culture flasks, by BD (1 mentions) Basic fibroblast growth factor (bfgf), by BD (1 mentions) Epidermal growth factor (egf), by BD (1 mentions) Neurocult ns a proliferation kit, by STEMCELL (1 mentions) Igf 1, by Merck Group (1 mentions) Basic fibroblast growth factor (bfgf), by Merck Group (1 mentions) Hct116, by American Type Culture Collection (1 mentions) Pre coated elisa kit, by Cusabio (1 mentions) Ifn γ, by BD (1 mentions) Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions) Stattic, by MedChemExpress (1 mentions) Phospho src, by Cell Signaling Technology (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Cck 8 solution, by Dojindo Laboratories (1 mentions) Mccoy s 5a media, by Thermo Fisher Scientific (1 mentions) Verteporfin, by MedChemExpress (1 mentions) Nobiletin, by MedChemExpress (1 mentions) Eagle s minimum essential medium emem, by Thermo Fisher Scientific (1 mentions) Phospho stat3, by Cell Signaling Technology (1 mentions)

5 protocols using igf 1

Induction of ESSC Proliferation and Differentiation

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E18 ESSCs were cultured using the NeuroCult NS-A Proliferation Kit and NeuroCult NS-A Differentiation Kit (rat) from Stem Cell Technologies, for the induction of proliferation and differentiation, respectively. The growth factor supplements consisting of insulin-like growth factor-1 (IGF-1, Sigma I 8779), leukaemia inhibitory factor (LIF, Sigma L 5158), epidermal growth factor (EGF, Sigma E 4127) and basic fibroblast growth factor (bFGF, Sigma F 0291) were purchased from Sigma. The concentrations of these growth factors used were 20 ng/mL for the EGF and bFGF, 100 ng/mL for the IGF-1 and 20 ng/mL for the LIF, in accordance with our recently published report [7 (link)]. The ESSCs were plated in T-25 culture flasks (BD Falcon) in triplicate, under five different conditions (Figure 1): group A (no growth factor); group B (EGF + bFGF); group C (EGF + bFGF + LIF); group D (EGF + bFGF + IGF-1); and group E (EGF + bFGF + LIF + IGF-1).

Pati S., Supeno N.E., Muthuraju S., Abdul Hadi R., Ghani A.R., Idris F.M., Maletic-Savatic M., Abdullah J.M, & Jaafar H. (2014). MicroRNA Profiling Reveals Unique miRNA Signatures in IGF-1 Treated Embryonic Striatal Stem Cell Fate Decisions in Striatal Neurogenesis In Vitro. BioMed Research International, 2014, 503162.

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Colorectal Cancer Cell Line Characterization

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Human colorectal adenocarcinoma cells, HT-29, Lovo, and HCT-116 were purchased from American Type Culture Collection (Manassas, VA, USA). Antibodies for ATF3 were purchased from Santa Cruz (Santa Cruz, CA, USA). Antibodies for GSK3β, PARP, actin, phospho-AKT (Ser473) and total AKT were purchased from Cell Signaling (Beverly, MA, USA). The trans-10, cis-12 CLA (t10, c12-CLA) was purchased from Natural Lipids (Hovdebygda, Norway). The t10, c12-CLA preparation was 94 % pure, with 2% cis-9, trans-11 isomer (c9, t11-CLA) and 3% other conjugated linoleic acid isomers. IGF-1 was purchased from BD Biosciences (Bedford, MA, USA). Human ATF3 promoter constructs were previously reported (Lee et al., 2010a (link)). All chemicals were purchased from Fisher Scientific, unless otherwise specified.

Kim K.J., Lee J., Park Y, & Lee S.H. (2015). ATF3 Mediates Anti-Cancer Activity of Trans-10, cis-12-Conjugated Linoleic Acid in Human Colon Cancer Cells. Biomolecules & Therapeutics, 23(2), 134-140.

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Airway Milieu Protein Analysis

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To determine the impact viral infection has on the airway milieu, we measured proteins by enzyme-linked immunosorbent assays. We measured the amount of Mucin 5AC in the apical wash of primary bronchial cells with a precoated ELISA kit from CUSABIO (Wuhan, China). IFNα (pbl IFN Source, Piscataway, NJ, USA), IFNγ and IGF-1 (BD Biosciences) were measured in undiluted BAL fluid following the manufacturer's protocols.

Samarasinghe A.E., Woolard S.N., Boyd K.L., Hoselton S.A., Schuh J.M, & McCullers J.A. (2014). The immune profile associated with acute allergic asthma accelerates clearance of influenza virus. Immunology and Cell Biology, 92(5), 449-459.

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Nobiletin Modulates Cancer Signaling

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Nobiletin (>99% purity) was purchased from MedChemExpress (Monmouth Junction, NY, USA); the CCK-8 solution was obtained from Dojindo Molecular Technologies, Inc. (Tokyo, Japan); fetal bovine serum was obtained from ZETA (San Francisco, CA, USA); trypsin and dimethyl sulfoxide (DMSO) were obtained from Thermo Fisher Scientific (Waltham, MA, USA); Eagle’s minimum essential medium (EMEM) and McCoy’s 5A media were obtained from Gibco (Gaithersburg, MD, USA). Antibodies against AKT, STAT3, SRC, YY1AP1, caspase 3, caspase 9, phospho-AKT, phospho-STAT3, phospho-YY1AP1, phospho-SRC, cleaved caspase 3, and cleaved caspase 9, as well as all secondary antibodies, were purchased from Cell Signaling Technology (Danvers, MA, USA). Stattic and Verteporfin were obtained from MedChemExpress. IGF1 was obtained from BD Bioscience (Shanghai, China).

Wei D., Zhang G., Zhu Z., Zheng Y., Yan F., Pan C., Wang Z., Li X., Wang F., Meng P., Zheng W., Yan Z., Zhai D., Lu Z, & Yuan J. (2019). Nobiletin Inhibits Cell Viability via the SRC/AKT/STAT3/YY1AP1 Pathway in Human Renal Carcinoma Cells. Frontiers in Pharmacology, 10, 690.

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Neural Crest Induction from Keratinocytes

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For induction into the NC fate, KC were cultured at a density of 10-15×10³ cells/ cm² in collagen type I coated dishes (10µg collagen type I per cm²; BD Biosciences) in the presence of neural crest induction medium (NCIM) comprising of basal medium (EBM-2 medium; Lonza) plus 2% (v/v) FBS, 10 µg per ml heparin, 100 µg per ml ascorbic acid and 0.5 µg per ml hydrocortisone, 1x Gentamicin/Amphotericin-B supplemented with 10 ng/ml fibroblast growth factor 2 (FGF2, BD Biosciences), 10 ng/ml Insulin like growth factor 1 (IGF1, BD Biosciences). Other induction factors and inhibitors tested were EGF, WNT1 (Life Technologies), Chir99021 (Tocris), NRG1 (Sigma), BMP4 (Gibco), and PD173074 (Cayman).

Bajpai V.K., Kerosuo L., Tseropoulos G., Cummings K.A., Wang X., Lei P., Liu B., Liu S., Popescu G., Bronner M.E, & Andreadis S.T. (2017). Reprogramming postnatal human epidermal keratinocytes toward functional neural crest fates. Stem cells (Dayton, Ohio), 35(5), 1402-1415.

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