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Dermatophagoides farina

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Dermatophagoides farina is a laboratory equipment product. It is a sample of the dust mite species Dermatophagoides farina, which is commonly used in allergy research and testing.

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Lab products found in correlation

Transit tko reagent, by Mirus Bio (2 mentions) Dermatophagoides pteronyssinus, by Stallergenes Greer (1 mentions)

5 protocols using dermatophagoides farina

1

Longitudinal Allergen Skin Prick Testing

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SPT was conducted at ages 18 months, 3, 5 and 8 years to major relevant allergens in Singapore, which included cow's milk, egg, peanut and house dust mites, Dermatophagoides pteronyssinus, Dermatophagoides farina (Greer Laboratories, Lenoir, NC, USA) and Blomia tropicalis. SPT was defined as positive at a timepoint if any of the allergens tested positive at the timepoint with an average wheal size of at least 3 mm.
Lau H.X., Chen Z., Chan Y.H., Tham E.H., Goh A.E., Van Bever H., Teoh O.H., Karnani N., Gluckman P.D., Tan K.H., Yap F.K., Godfrey K.M., Eriksson J.G., Chong Y.S., Lee B.W., Shek L.P, & Loo E.X. (2022). Allergic sensitization trajectories to age 8 years in the Singapore GUSTO cohort. The World Allergy Organization Journal, 15(7), 100667.
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2

HDM-Induced Asthma Model in Mice

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Eight-week-old female WT C57BL/6 mice were sensitized subcutaneously with HDM (100 μg per mouse; Dermatophagoides farina, Greer Laboratories) in complete Freund’s adjuvant (CFA) on day 0 and subsequently challenged (intranasally) with HDM (100 μg per mouse) on day 14. AHR measurement, BAL cell counting, and tissue collection were performed 24 hours after the last HDM challenge. The HDM + A18 group of mice were injected (intraperitoneally) with A18 (120 μg per mouse) on days 13 and 14 and were also administered with A18 (60 μg per mouse) (intranasally) 1 hour before challenge.
Liu C., Zhu L., Fukuda K., Ouyang S., Chen X., Wang C., Zhang C.J., Martin B., Gu C., Qin L., Rachakonda S., Aronica M., Qin J, & Li X. (2017). The flavonoid cyanidin blocks binding of the cytokine interleukin-17A to the IL-17RA subunit to alleviate inflammation in vivo. Science signaling, 10(467), eaaf8823.
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3

Intranasal Aptamer Delivery Alleviates HDM-Induced Asthma

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Eight-week-old female WT C57BL/6 mice were sensitized sub-cutaneously
with HDM (100 mg per mouse; Dermatophagoides farina, Greer Laboratories) in
complete Freund’s adjuvant (CFA) on day 0 and subsequently challenged
(intranasally) with HDM (100 mg per mouse) on day 14. One hour before last HDM
challenge mice were administered intranasally with either SBE WT or SBE mutant C
aptamer mixed with 5 μl of TransIT-TKO reagent (Mirus Bio) and PBS in a
total volume of 40 μl. We equally divided aptamer mix and into two
nostrils and applied with a micropipette. Each mouse received 5 nmol of aptamer.
BAL cell counting, and tissue collection were performed 24 hours after the last
HDM challenge.
For monitoring aptamer delivery into trachea fluorescently-labeled SBE
WT aptamers were intranasally delivered to tamoxifen treated LSL-Act1-HA
knock-in Cre-ERT2 mice (see Intradermal
injection).
Herjan T., Hong L., Bubenik J., Bulek K., Qian W., Liu C., Li X., Chen X., Yang H., Ouyang S., Zhou H., Zhao J., Komireddy V., Cockman E., Aronica M., Asosingh K., Licatalosi D.D., Qin J., Fox P.L., Hamilton T.A., Driscoll D, & Li X. (2018). IL-17 receptor-associated adaptor Act1 directly stabilizes mRNAs to mediate IL-17 inflammatory signaling. Nature immunology, 19(4), 354-365.
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4

Intranasal Aptamer Delivery Alleviates HDM-Induced Asthma

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Eight-week-old female WT C57BL/6 mice were sensitized sub-cutaneously
with HDM (100 mg per mouse; Dermatophagoides farina, Greer Laboratories) in
complete Freund’s adjuvant (CFA) on day 0 and subsequently challenged
(intranasally) with HDM (100 mg per mouse) on day 14. One hour before last HDM
challenge mice were administered intranasally with either SBE WT or SBE mutant C
aptamer mixed with 5 μl of TransIT-TKO reagent (Mirus Bio) and PBS in a
total volume of 40 μl. We equally divided aptamer mix and into two
nostrils and applied with a micropipette. Each mouse received 5 nmol of aptamer.
BAL cell counting, and tissue collection were performed 24 hours after the last
HDM challenge.
For monitoring aptamer delivery into trachea fluorescently-labeled SBE
WT aptamers were intranasally delivered to tamoxifen treated LSL-Act1-HA
knock-in Cre-ERT2 mice (see Intradermal
injection).
Herjan T., Hong L., Bubenik J., Bulek K., Qian W., Liu C., Li X., Chen X., Yang H., Ouyang S., Zhou H., Zhao J., Komireddy V., Cockman E., Aronica M., Asosingh K., Licatalosi D.D., Qin J., Fox P.L., Hamilton T.A., Driscoll D, & Li X. (2018). IL-17 receptor-associated adaptor Act1 directly stabilizes mRNAs to mediate IL-17 inflammatory signaling. Nature immunology, 19(4), 354-365.
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5

Murine Model of Allergic Airway Inflammation

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Eight-week-old female C57BL/6 mice were sensitized intranasally with HDM (200 μg in 50 μL saline per mouse; Dermatophagoides farina, Greer Laboratories) on day 0 and day 1. These mice were subsequently challenged (intranasally) with HDM (30 μg in 50 μL saline per mouse) on day 14 for five consecutive days. The HDM + (Ber-NPs) group and the HDM + (PM@Ber-NPs) group of mice were administered intranasally with Ber (2 mg/kg body weight) 1 h before HDM challenge from day 14 to day 21. Bronchoalveolar lavage fluid (BALF), sera, and lung samples were collected 48 h after the last HDM challenge.
Jin H., Li J., Zhang M., Luo R., Lu P., Zhang W., Zhang J., Pi J., Zheng W., Mai Z., Ding X., Liu X., Ouyang S, & Huang G. (2021). Berberine-Loaded Biomimetic Nanoparticles Attenuate Inflammation of Experimental Allergic Asthma via Enhancing IL-12 Expression. Frontiers in Pharmacology, 12, 724525.
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