Hiscript 3 1st strand cdna synthesis kits gdna wiper

The SARS-CoV-2 nucleic acid standards were diluted to 100 copies per microliter (copies/μL) in nuclease-free sterile water. The Complementary DNA (cDNA) of SARS-CoV-2 standards and clinical samples were synthesized using a CFX96 C1000 thermal cycler (Bio-Rad Laboratories) and HiScript III 1st Strand cDNA Synthesis kits (+gDNA wiper) (R312, Vazyme Biotech Co. Ltd., Nanjing, China). Dilations of the SARS-CoV-2 standards of 1, 10, and 100 copies/μL were prepared through dilution of the cDNA libraries of the SARS-CoV-2 standards according to the original concentration, and then confirmed by detecting the N and ORF1ab regions of the SARS-CoV-2 genome using qRT-PCR. The qPCR tests were performed at our laboratory using the AceQ qPCR Probe Master Mix (Q112, Vazyme Biotech Co. Ltd., Nanjing, China) on an ABI StepOnePlus real-time PCR system. qRT-PCR tests of clinical specimens were routinely performed at the hospital using 2019-nCoV nucleic test kits (Bojie Ltd., Shanghai, China).

The SARS-CoV-2 nucleic acid standards were diluted to 100 copies per microliter (copies/μL) in nuclease-free sterile water. The Complementary DNA (cDNA) of SARS-CoV-2 standards and clinical samples were synthesized using a CFX96 C1000 thermal cycler (Bio-Rad Laboratories) and HiScript III 1st Strand cDNA Synthesis kits (+gDNA wiper) (R312, Vazyme Biotech Co. Ltd., Nanjing, China). Dilations of the SARS-CoV-2 standards of 1, 10, and 100 copies/μL were prepared through dilution of the cDNA libraries of the SARS-CoV-2 standards according to the original concentration, and then confirmed by detecting the N and ORF1ab regions of the SARS-CoV-2 genome using qRT-PCR. The qPCR tests were performed at our laboratory using the AceQ qPCR Probe Master Mix (Q112, Vazyme Biotech Co. Ltd., Nanjing, China) on an ABI StepOnePlus real-time PCR system. qRT-PCR tests of clinical specimens were routinely performed at the hospital using 2019-nCoV nucleic test kits (Bojie Ltd., Shanghai, China).