Gcms qp5050 spectrometer

Potassium tert-butoxide, 4-methoxybenzenethiol (3), benzenethiol (4), naphthalene-2-thiol (11) and potassium diphenylphosphide solution (0.5 M in THF) are commercially available and used as received. Methyl-4-iodocubane-1-carboxylate (1) and 1,4-diiodocubane (2) were synthesized according to ref. 18 (link). DMSO is Carlo Erba and stored under molecular sieves (4 Å). ¹H NMR and ¹³C NMR spectra were recorded on a 400 MHz Bruker nuclear magnetic resonance spectrometer. HR-MS were recorded on a Bruker, MicroTOF Q II equipment, operated with an ESI source in (positive/negative) mode, using nitrogen as nebulizing and drying gas and sodium formate 10 mM as internal standard. Gas chromatographic analyses were performed on a Varian 3900 GC with flame ionization detector on a FactorFour capillary column (VF-5 MS, 30 m, 0.32 mm, 0.25 micron). GC-MS analyses were carried out on a Shimadzu GC-MS QP5050 spectrometer, employing a 30 m, 0.32 mm, 0.25 micron, DB-5 MS column. Irradiation was performed in a reactor equipped with two 400 W lamps (Philips model Master HPI-T Plus, air- and water-cooled). The Fig. SI-1 in ESI† shows the spectrum of the lamps. HPLC analyses were carried out on a Waters 1525 Binary HPLC Pump connected to a Waters 2998 Photodiode Array Detector, and employing an Agilent Zorbax Eclipse XDB-C18 Analytical column (4.6 × 150 mm, 5 μm).

AM was extracted from the stem bark of C. arborescens, wild trees growing in Malaysia. A voucher specimen was deposited at the Herbarium, Department of Biology, University Putra Malaysia. Hexane, chloroform and methanol extracts were collected. The characterization and identification of AM have been done using JOEL ECA-400 spectrometer and mass spectra data was recorded using a Shimadzu GCMS-QP5050 spectrometer (Sidahmed et al., 2013 (link)). The purity of AM was confirmed to be 100% by HPLC-LC 20 AD (Shimadzu, Japan) using reversed-phase C18, 2.5 mm column from Waters with 10% acetonitrile in water. One peak has been observed between 7–7.5 min. The detection of the peak was at 245 nm wavelength using a Gilson absorbance detector (UV–VIS165 Gilson USA).