Sds page gel kit

Yeast extract and tryptone were purchased from Oxoid (Shanghai, China). Pyridoxal-5’-phosphate (PLP), pyruvate and R-1-phenylethylamine (1-(R)-PEA) were purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Choline chloride (ChCl), urea, ethylene glycol (EG), propylene glycol (PG), glycerol, formate, kanamycin sulphate, isopropyl–β-d-thiogalactopyranoside (IPTG), sodium dihydrogen phosphate, disodium phosphate, imidazole, sodium chloride, Ni-NTA Sefinose™ Resin (Settled Resin), Modified Bradford Protein Assay kit and SDS PAGE gel kit were obtained from Sangon (Shanghai, China). Amicon^®® Ultra Filter units were obtained from Sigma Aldrich (Shanghai, China). All chemicals were of analytical grade. The transaminase genes from Aspergillus terreus, which were previously transformed in E. coli BL21 (DE3) pRSF-D-AAO competent cells, were stored in our laboratory at −80 °C. The plasmids and the E. coli BL21 (DE3) pRSF-D-AAO competent cells were constructed by Anhui General Biology Company (Anhui, China).

The At-ATA cDNA from Aspergillus terreus sequence, including the NcoI and XhoI restriction sites, was synthesized by General Biosystems (Chuzhou, China), and the plasmid pET-28a(+) was used for gene cloning and DNA sequencing. All PCR primers were synthesized by Qingke Biology Co., Ltd. (Hangzhou, China). PrimeSTAR^® Max DNA polymerase was obtained from Takara Biotechnology (Dalian, China) for the polymerase chain reaction (PCR). Dpn I, Yeast extract and tryptone were obtained from Thermo Fisher Scientific (Shanghai, China). Dimethyl sulfoxide (DMSO), 1-(R)-PEA and pyruvate were obtained from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). NaCl, NaH₂PO₄, Na₂HPO₄, NaOH, DNA ladder, protein marker, protein loading buffer, kanamycin sulfate, isopropyl-β-d-thiogalactoside (IPTG), Ni-NTA Sefinose (TM) Resin (Settled Resin) kit, SDS-PAGE gel kit, and Modified Bradford Protein Assay Kit were obtained from Sangon (Shanghai, China). E. coli BL21(DE3) Chemically Competent Cell, EasyPure^® HiPure Plasmid MaxiPrep Kit, EasyPure^® Quick Gel Extraction Kit and EasyPure^® PCR Purification Kit were purchased from TransGen Biotech (Beijing, China).