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Corticosterone elisa

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The Corticosterone ELISA kit is a quantitative enzyme-linked immunosorbent assay designed to measure corticosterone levels in various sample types, including serum, plasma, and tissue extracts. The kit uses a competitive immunoassay principle to determine the concentration of corticosterone in the samples.

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Lab products found in correlation

Synergy 2 multi mode plate reader, by Agilent Technologies (2 mentions) Ultrasensitive mouse insulin elisa, by Mercodia (1 mentions) Triglyceride determination kit, by Merck Group (1 mentions) Quantikine elisa mouse rat fgf 21, by R&D Systems (1 mentions) Quantikine elisa mouse adiponectin acrp30, by R&D Systems (1 mentions) Synergy ht, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Corticosterone ELISA kit (103 citations) Corticosterone (25 citations) Enzo Corticosterone ELISA kit (2 citations) Corticosterone Elisa Kit ADI-900-097 (2 citations)

8 protocols using corticosterone elisa

1

Plasma Hormone Quantification and Hepatic Triglycerides

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Hormone concentrations in plasma sampled at the time of killing were measured with the following commercially available kits: insulin, Ultrasensitive Mouse Insulin ELISA (Mercodia, Uppsala, Sweden); leptin, Metabolism & Endocrinology Multiplex Assays (Luminex, Merck Millipore, Burlington, MA, USA); adiponectin, Quantikine ELISA Mouse Adiponectin/Acrp30 (R&D Systems, MN, USA); fibroblast growth factor-21 (FGF-21), Quantikine ELISA Mouse/Rat FGF-21 (R&D Systems); corticosterone, Corticosterone ELISA (Enzo Life Sciences, Farmingdale, NY, USA). Hepatic triglycerides were determined after extraction according to Folch (32 (link)) using the Triglyceride Determination Kit from Sigma Aldrich (St. Louis, MO, USA).
Abu Eid S., Hackl M.T., Kaplanian M., Winter M.P., Kaltenecker D., Moriggl R., Luger A., Scherer T, & Fürnsinn C. (2018). Life Under Hypoxia Lowers Blood Glucose Independently of Effects on Appetite and Body Weight in Mice. Frontiers in Endocrinology, 9, 490.
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2

Serum Corticosterone Measurement Protocol

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To measure serum corticosterone concentration, blood (~200 μL) was collected by decapitation (for terminal corticosterone measurement) or by submandibular vein puncture (for serial corticosterone measurement). The blood was allowed to coagulate at room temperature for 90 min and was centrifuged at 2000 × g for 15 min. Serum was collected and samples were stored at −20°C until they were shipped to the University of Virginia Center for Research in Reproduction Ligand Assay and Analysis Core (terminal experiments), where corticosterone levels were measured by 125I corticosterone radioimmunoassay, or until they were assayed in-house using a corticosterone ELISA (Enzo Life Sciences, Farmingdale, NY) according to the manufacturer’s instructions (for serial measurement experiments).
Jiang Z., Chen C., Weiss G.L., Fu X., Stelly C.E., Sweeten B.L., Tirrell P.S., Pursell I., Stevens C.R., Fisher M.O., Begley J.C., Harrison L.M, & Tasker J.G. (2022). Stress-induced glucocorticoid desensitizes adrenoreceptors to gate the neuroendocrine response to somatic stress in male mice. Cell reports, 41(3), 111509.
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3

Plasma Corticosterone in Stress Response

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In order to determine whether physiological stress responses differed after contextual re-exposure in males exposed to either direct social defeat (intruders) or witness stress, plasma corticosterone was measured using a Corticosterone ELISA (Enzo Life Sciences, Farmingdale, NY). Plasma was diluted 1:40 and run according to manufacturer protocol. Plates were read using a Synergy 2 Multi-Mode plate reader (Bio Tek, Winooski, VT) with Gen5 software (Bio Tek, Winooski, VT).
Finnell J.E., Lombard C.M., Padi A.R., Moffitt C.M., Wilson L.B., Wood C.S, & Wood S.K. (2017). Physical versus psychological social stress in male rats reveals distinct cardiovascular, inflammatory and behavioral consequences. PLoS ONE, 12(2), e0172868.
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4

Corticosterone Quantification from Blood

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Trunk blood was collected, then allowed to sit overnight at 4 °C. The following day, samples were centrifuged at 3,000 rcf, then the supernatant was collected and stored at −80 °C until use. All samples were run in duplicate using the Corticosterone ELISA (Enzo) and concentration was determined as percentage bound using a standard curve ranging from 32 – 20,000 pg mL−1.
Ota K.T., Liu R.J., Voleti B., Maldonado-Aviles J.G., Duric V., Iwata M., Dutheil S., Duman C., Boikess S., Lewis D.A., Stockmeier C.A., DiLeone R.J., Rex C., Aghajanian G.K, & Duman R.S. (2014). REDD1 is essential for stress-induced synaptic loss and depressive behavior. Nature medicine, 20(5), 531-535.
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5

Quantifying Stress Hormone Levels

Cited 1 time
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To determine differences in circulating stress hormones following the acute defeat challenge, plasma corticosterone was measured using a Corticosterone ELISA (Enzo Life Sciences, Farmingdale, NY) as previously published (Finnell et al., 2017b (link)). Briefly, plasma was diluted 1:40 and run according to manufacturer protocol. Plates were read using a Synergy 2 Multi-Mode plate reader (Bio Tek, Winooski, VT) with Gen5 software (Bio Tek, Winooski, VT).
Finnell J.E., Moffitt C.M., Hesser L.A., Harrington E., Melson M.N., Wood C.S, & Wood S.K. (2019). The contribution of the locus coeruleus-norepinephrine system in the emergence of defeat-induced inflammatory priming. Brain, behavior, and immunity, 79, 102-113.
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6

Circadian Regulation of Corticosterone

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All sacrifice started at 10:00 am. The mice of each group were anesthetized at the same time with 2.5% isoflurane followed by decapitation. Truckal blood was collected, set on ice for 30 min, and centrifuged. Corticosterone was measured using a corticosterone ELISA (Enzo). Thymus and spleen were dissected, weighed, and immediately processed for flow cytometry.
Xiao R., Bergin S.M., Huang W., Mansour A.G., Liu X., Judd R.T., Widstrom K.J., Queen N., Wilkins R., Siu J.J., Ali S., Caligiuri M.A, & Cao L. (2018). Enriched environment regulates thymocyte development and alleviates experimental autoimmune encephalomyelitis in mice. Brain, behavior, and immunity, 75, 137-148.
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7

Plasma Corticosterone Quantification

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Corticosterone quantification of plasma (15 μL) collected from trunk blood at the sacrifice was performed using a corticosterone ELISA (Enzo Life Sciences) and was performed according to the manufacturer's instructions and was analysed as previously described (Bastiaanssen et al., 2021 (link); Anand Gururajan et al., 2022 (link)). A multi-mode plate reader (Synergy HT, BioTek Instruments) was used to quantify light absorbance in the assay, at 405 nm. Only data derived from duplicates with <15% CV were included in the analysis. Concentrations of plasma corticosterone were expressed in ng/mL. Limit of detection is represented in Supplementary Table 1.
Cruz-Pereira J.S., Moloney G.M., Bastiaanssen T.F., Boscaini S., Tofani G., Borras-Bisa J., van de Wouw M., Fitzgerald P., Dinan T.G., Clarke G, & Cryan J.F. (2022). Prebiotic supplementation modulates selective effects of stress on behavior and brain metabolome in aged mice. Neurobiology of Stress, 21, 100501.
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8

Corticosterone Quantification from Blood

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Trunk blood was collected, then allowed to sit overnight at 4 °C. The following day, samples were centrifuged at 3,000 rcf, then the supernatant was collected and stored at −80 °C until use. All samples were run in duplicate using the Corticosterone ELISA (Enzo) and concentration was determined as percentage bound using a standard curve ranging from 32 – 20,000 pg mL−1.
Ota K.T., Liu R.J., Voleti B., Maldonado-Aviles J.G., Duric V., Iwata M., Dutheil S., Duman C., Boikess S., Lewis D.A., Stockmeier C.A., DiLeone R.J., Rex C., Aghajanian G.K, & Duman R.S. (2014). REDD1 is essential for stress-induced synaptic loss and depressive behavior. Nature medicine, 20(5), 531-535.
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