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232 protocols using sas university edition

1

Lignin-based Flexible Foam Characterization

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The results were analyzed using SAS University Edition software to check the analysis of variance (ANOVA) and Tukey’s honestly significant difference (Tukey’s HSD) test with a 95% confidence level to calculate the significant differences between the means. The correlations between lignin properties and lignin-based flexible foam performance were also analyzed using the Pearson correlation matrix in SAS University Edition software.
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2

Respiratory Support Risk Factors

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Continuous variables were checked for normality with the Kolmogorov–Smirnov test. Differences between continuous variables were investigated using the Student t-test for independent samples and the Mann–Whitney U test according to distribution. Spearman correlation analysis was performed to investigate the correlation between liver enzymes, inflammatory markers, and respiratory parameters. Univariate logistic regression analyses were performed to identify candidate variables for multivariate analysis in order to estimate the risk of respiratory support. Variables showing association (p < 0.05) with respiratory support were included in the multivariate analysis. Chi-square tests were performed for differences in categorical variables. Continuous variables are expressed as medians (interquartile range, IQR), whereas categorical variables are expressed as frequencies. All the analyses were performed using SAS® University Edition (SAS Institute Inc., Cary, NC, USA). Values of p < 0.05 were considered statistically significant.
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3

Statistical Analysis of Clinical Scores and Viral Data

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Mean cumulative clinical scores were analysed using linear mixed model, with calf as random effect. Viraemia and serological results were compared using two-way ANOVA with repeated measures. RNA detection in organs at necropsy, frequencies and proportions were compared with Fisher’s Exact Test for count data [25 ]. For all tests, P values <0.05 were considered significant. In case of multiple comparisons, a Bonferroni correction was applied to reduce the risk of type I error (conservative approach) and a Holm correction was applied when more than four comparisons had to be tested. Statistical analyses were performed using the R software/environment (R-3.1.2, R Foundation for Statistical Computing, [26 ]) and SAS software, Version 9.3 TS level 1M2 of the SAS System for Unix, and SAS University Edition (SAS Institute, Cary, NC, USA).
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4

Antimicrobial Efficacy of Acids

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Results of antimicrobial efficiencies, including growth inhibition zone diameters and the lowest concentrations of MIC and MBC, of the acids among specific pathogens were described as means ± standard error of the mean (SEM). The antimicrobial efficiencies data were tested for their normal distribution for each pathogen. Logarithm transformation was applied for non-normally distributed data. Differences in growth inhibition zone, MIC and MBC among the four acids for each pathogen were calculated by applying the one-way analysis of variance (ANOVA), and Tukey’s multiple-range tests, SAS® University Edition, were used for pairwise comparison. Significance was defined as p < 0.05.
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5

Urinary Biomarkers and Kidney Outcomes

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The primary outcomes were edema status (yes/no) and eGFR at time of biopsy. Edema status was extracted from the notes in the EMR. All urinary markers (protein/creatinine, albumin/creatinine, plasminogen/creatinine) were log-transformed to approximate normal distribution. Correlations between log-transformed biomarkers and relevant characteristics were assessed using Spearman’s partial correlations. Boxplots that denoted the median, IQR, minimum and maximum were used to visualize the distribution of each urinary biomarker with edema status yes vs. no and eGFR < 60 vs. 60. The association for each log-transformed biomarker with the kidney-related clinical characteristic was evaluated by univariable and multivariable logistic regression for edema status and with univariable and multivariable linear regression for eGFR (adjusted for age, gender, race/ethnicity, RASi or diuretic use, and eGFR, as appropriate) using SAS® University Edition (Cary, NC).
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6

Tumor Analysis via Statistical Methods

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Statistical analysis was carried out using SAS University Edition (SAS Institute, Inc, Cary, NC). Descriptive statistics are presented as mean ± SEM. One-way ANOVA was used to compare differences in tumor variables between groups. Differences in apoptosis, proliferation, gene expression, citrate synthase activity and, densitometric values were evaluated using t-tests. For all tests, p < 0.05 was considered statistically significant.
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7

Exploring Health Disparities in RDS Samples

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Existing univariate and multivariable analysis methods for examining associations are not directly transferable to RDS samples because random sampling assumptions are not satisfied. Bivariate associations between exposure to discrimination by a healthcare provider and the outcome of an unmet health need were explored using unadjusted odds ratios. Following this, stratified odds ratios were calculated for each social determinant of health, including access to a regular healthcare provider and socio-demographic factors (gender, age, education level, employment status, food security, mobility in the past 12 months, and income level).
RDS weights were calculated, using the RDS-II weighting estimator developed by Volz and Heckathorn, along with wave number, for each eligible participant through the RDS Analyst program, powered by the statistical package R (Volz and Heckathorn 2008 ; Handcock et al. 2014 ). Prevalence estimates and unstratified and stratified odds ratios for the associations of interest were calculated with 95% confidence intervals in SAS® University Edition (SAS Institute Inc. SAS® University Edition for OS X 2016 ).
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8

Dietary Effects on Urinary Biomarkers

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All data were analyzed with PROC MIXED including in the model the effects of diet and feeding duration as fixed effects for repeated measures using SAS University Edition (SAS Institute Inc., Cary, NC). For measurement comparisons, we used the least mean square algorithm and performed Tukey–Kramer multiple-comparison adjustment. We also used linear regression to analyze the correlation between UCrn and UUN in each diet in Experiment 1. Regression line slopes were compared with the analysis of covariance. For all analyses, the level of statistical significance was set at 5% (P < 0.05). Values were presented as mean ± standard error.
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9

Protein Expression and Behavioral Analysis

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All quantitative data were expressed as mean ± SEM. Western blot data were analyzed using unpaired t-test in GraphPad Prism6 (GraphPad Software, CA, USA). Behavioral data were analyzed using unpaired t-test, paired t-test, or two-way repeated measures ANOVAs in SAS University Edition (SAS institute, NC, USA). P-values < 0.05 were considered to denote significance.
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10

Testicular Biometry and Sperm Quality in Bulls

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For each bull, averages of the data from the left and right testicles for linear measurements, as well as for histopathology, were used. The data of testicular biometry, sperm analysis, and testosterone concentration were initially tested for normality using the Shapiro-Wilk test. Analyses considered the main effects of treatment (CG, G1, and G2), time (days), and their interactions. The SAS GLIMMIX procedure with a REPEATED statement was used to account for auto-correlation between sequential measures (SAS University Edition, SAS Institute Inc., Cary, NC, USA). The model was adjusted for the type of distribution of each endpoint. Differences between means were compared using the Tukey test. Data of weight and carcass traits was analyzed using the GLIMMIX procedure of the SAS. The grades of seminiferous tubule degeneration were compared among groups using the Kruskal-Wallis non-parametric test. Associations between the degree of degeneration of the seminiferous tubules and other testicle parameters at day 90 were calculated using the Pearson’s correlation method. Results are presented as mean ± S.E.M. A P value lower than 0.05 indicated statistical significance.
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