Fitc conjugated goat anti mouse igm

Manufactured by Thermo Fisher Scientific

Sourced in Germany, United States

FITC-conjugated goat-anti-mouse IgM is a secondary antibody that binds to mouse IgM antibodies. The FITC (fluorescein isothiocyanate) conjugate allows for the fluorescent detection of the target IgM antibodies.

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Lab products found in correlation

Facscanto 2, by BD (2 mentions) Enoxaparin, by Sanofi (2 mentions) Dalteparin, by Pfizer (2 mentions) Cd1a apc mouse igg1, by BD (2 mentions) Heparan sulfate clone f58 10e4, by AMS Biotechnology (2 mentions) Af488 conjugated donkey anti mouse igg2b, by Thermo Fisher Scientific (2 mentions) Apc conjugated cd1a, by BD (1 mentions) Dc sign fitc, by R&D Systems (1 mentions) Cd3 apc fire750, by BioLegend (1 mentions) Cd11c apc, by BioLegend (1 mentions) Cd14 apc cy7, by BD (1 mentions) Cd11c apc cy7, by BioLegend (1 mentions) Af488 conjugated donkey anti rabbit, by Thermo Fisher Scientific (1 mentions) Cd80 pe, by BD (1 mentions) Cd86 fitc, by BD (1 mentions) Cd83 apc, by BD (1 mentions) Mouse igg2a, by Abcam (1 mentions) Hla dr fitc clone g46 6, by BD (1 mentions) Mouse igg2b, by BD (1 mentions)

Spelling variants of this product

Goat anti-mouse FITC (12 citations) Goat anti-mouse IgM (10 citations) Anti-IgM-FITC (9 citations) IgM-FITC (7 citations) FITC-conjugated goat anti-mouse (5 citations) FITC-conjugated anti-IgM (3 citations)

4 protocols using fitc conjugated goat anti mouse igm

Zika Virus Detection in Immune Cells

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Cells were fixed with paraformaldehyde (PFA) and treated with either BSA (extracellular staining) or BSA/Saponin to measure intracellular staining. The following antibodies were used to detect Zika virus: anti-Flavivirus 4g2 mouse IgG2a (NovusBio), anti-Flavivirus 4g2 monoclonal rabbit (Absolute Antibody), anti-Zika virus monoclonal Rabbit (Genetex).
All other antibodies were anti-human: DC-SIGN mouse IgG1 (AZN-D1), anti-langerin mouse IgG1 (10E2) both in house made, PE conjugated CD207 (langerin), APC conjugated CD1a (BD Biosciences), CD86-FITC (BD Pharmingen), CD80-PE (BD Pharmingen), CD83-APC (BD Pharmingen), DC-SIGN-FITC (R&D systems), CD3-APC/Fire750 (Biolegend), CD11c-APC (Biolegend), PEcy7-HLA-DR (BD Pharmingen), APCcy7-CD14 (BD Biosciences), APCcy7-CD11c (Biolegend),APC-AXL (Thermofisher, PE-MerTK (Thermofisher) and anti-Tyro3 (Thermofisher). For secondary detection the following antibodies were used: AF488-conjugated goat anti-mouse IgG2a (Invitrogen), AF647-conjugated donkey anti-rabbit (Thermofisher), FITC-conjugated goat-anti-mouse IgM (Invitrogen), AF488-conjugated donkey anti-rabbit (Thermofisher).
Flow cytometric analyses were performed on a BD FACS Canto II (BD Biosciences) and data was analysed using FlowJo V10 software (TreeStar).

Eder J., Zijlstra-Willems E., Koen G., Kootstra N.A., Wolthers K.C, & Geijtenbeek T.B. (2023). Transmission of Zika virus by dendritic cell subsets in skin and vaginal mucosa. Frontiers in Immunology, 14, 1125565.

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Quantifying Tumor-Specific IgM Antibodies

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For determination of tumor cell-specific IgM antibodies, indirect immunofluorescence analysis was used. Mice sera were thawed on ice and 1 μl of the respective serum sample was co-incubated with 1 × 10⁵ viable CT26 cells for 1 h at 4°C. Thereafter, cells were washed with PBS/10% FBS. The amount of bound antibodies was analyzed by adding staining solution [5.8 μg/ml FITC-conjugated goat anti-mouse IgM (Invitrogen, Darmstadt, Germany)] for 1 h at 4°C in the dark. After washing, cells were resuspended in PBS/10% FBS. Using flow cytometry, the mean fluorescence intensity of CT26 cells per sample was analyzed and equated with the tumor cell-specific IgM antibody level in the serum.

Frey B., Rückert M., Weber J., Mayr X., Derer A., Lotter M., Bert C., Rödel F., Fietkau R, & Gaipl U.S. (2017). Hypofractionated Irradiation Has Immune Stimulatory Potential and Induces a Timely Restricted Infiltration of Immune Cells in Colon Cancer Tumors. Frontiers in Immunology, 8, 231.

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SARS-CoV-2 Antibodies and Heparin Interactions

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The following antibodies were used (all anti‐human): ACE2 (R&D), (Heparan Sulfate (clone F58‐10E4) (Amsbio), digested Heparan (clone F69‐3G10) (Amsbio), CD1a‐APC mouse IgG1 (BD Biosciences, San Jose, CA, USA), CD207‐PE (langerin) mouse IgG1 (#IM3577), PerCP‐Cy™5.5‐conjugated mouse IgG1 EPCAM 347199) (BD Bioscience), PE‐conjugated mouse IgG1 E‐Cadherin (FAB18381P) (R&D Systems), APCcy‐conjugated mouse IgG1 CD45 (557833) (BD Bioscience), APC‐conjugated CD14 (21620146sp) (Immunotools), PE‐conjugated mouse IgG1 CD11b (101208) (Biolegend). FITC‐conjugated goat‐anti‐mouse IgM (#31992) (Invitrogen), AF488‐conjugated donkey‐anti‐mouse IgG2b (Invitrogen). Flow cytometric analyses were performed on a BD FACS Canto II (BD Biosciences). Data were analyzed using FlowJo vX.0.7 software (TreeStar).
The following reagents were used: unfractionated (UF) heparin, 5.000 IE/ml (LEO). low molecular weight heparins (LMWH): dalteparin, 10.000 IE anti‐Xa/ml (Pfizer), tinzaparin, 10.000 IE anti‐X1/0.5 ml (LEO), enoxaparin, 6000 IE (60 mg)/0.6 ml (Sanofi), nadroparin, 9.500 IE anti‐XA/ml (Aspen). Heparinase III from Flavobacterium heparium, EC 4.2.2.8, Batch 010, (Amsbio). Biotinylated SARS‐CoV‐2 S protein as well as neutralizing and non‐neutralizing COVA antibodies was generated as described previously (Brouwer et al, 2020 (link)).

Bermejo‐Jambrina M., Eder J., Kaptein T.M., van Hamme J.L., Helgers L.C., Vlaming K.E., Brouwer P.J., van Nuenen A.C., Spaargaren M., de Bree G.J., Nijmeijer B.M., Kootstra N.A., van Gils M.J., Sanders R.W, & Geijtenbeek T.B. (2021). Infection and transmission of SARS‐CoV‐2 depend on heparan sulfate proteoglycans. The EMBO Journal, 40(20), e106765.

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Multiparametric Flow Cytometry for Glycosaminoglycans

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The following antibodies were used (all anti-human): Heparan Sulfate (clone F58-10E4) (Amsbio), digested Heparan (clone F69-3G10) (Amsbio), Syndecan 1 (DL-101) (Santa Cruz), Syndecan 2-FITC (H-7) (Santa Cruz), Syndecan 3 (M-300) (Santa Cruz), Syndecan 4 (clone F94-8G3), CD207-PE (langerin) mouse IgG1 (#IM3577) (BeckmanCoulter, USA), CD1a-APC mouse IgG1 (BD Biosciences, San Jose, CA, USA) CD1a-PE (clone SK9) mouse IgG2b (BD Bioscience), HLA-B27-FITC (clone HLA-ABC-m3), mouse IgG2a (Abcam), HLA-DR-FITC (clone G46-6), mouse IgG2b (BD Bioscience), CD80-PE, mouse IgG1 (BD Pharmingen), CD83-PE, mouse IgG1 (eBioscience), CD86-FITC, mouse IgG1 (BD Pharmingen), FITC-conjugated goat-anti-mouse IgM (#31992) (Invitrogen), AF488-conjugated goat-anti-mouse IgG1 (#A21121) (Invitrogen), AF488-conjugated donkey-anti-mouse IgG2b (Invitrogen).
The following reagents were used: Unfractionated (UF) heparin, 5.000 I.E./ml (LEO). Low Molecular Weight (LMW) heparins: dalteparin, 10.000 IE anti-Xa/ml (Pfizer), tinzaparin, 10.000 IE anti-X1/0.5ml (LEO), enoxaparin, 100 mg/ml (Sanofi). 4-Nitrophenyl β-D-xylopyranoside (PNP-Xyl, 2001-96-9) (SigmaAldrich). Heparinase III from Flavobacterium heparium, EC 4.2.2.8, Batch 010 (Amsbio). 123Count eBeads, REF# 01-1234-42, LOT# E133305, 1.011.000 eBeads/ml (eBioscience).

Nijmeijer B.M., Eder J., Langedijk C.J., Kaptein T.M., Meeussen S., Zimmermann P., Ribeiro C.M, & Geijtenbeek T.B. (2020). Syndecan 4 Upregulation on Activated Langerhans Cells Counteracts Langerin Restriction to Facilitate Hepatitis C Virus Transmission. Frontiers in Immunology, 11, 503.

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