Statistical analyses were performed by using GraphPad Prism 5.0 for Windows (GraphPad Software Inc.). Data distribution was checked, and statistical significance was evaluated by Mann–Whitney U test. All data are expressed as the mean ± SEM. P < 0.05 was considered statistically significant.
Prism 5.0 for windows
Manufactured by GraphPad
Sourced in United States
GraphPad Prism 5.0 for Windows is a data analysis and graphing software. It provides tools for a variety of data analysis tasks, including curve fitting, statistical analysis, and the creation of scientific graphs and charts.
Automatically generated - may contain errors
Lab products found in correlation
Spss software for windows version 21, by IBM (2 mentions)
Spss 17.0 for window, by IBM (2 mentions)
Spss v22, by IBM (2 mentions)
Spss version 27, by IBM (1 mentions)
Spss statistics for window, by IBM (1 mentions)
Spss software version 21, by IBM (1 mentions)
Image studio lite software, by LI COR (1 mentions)
Genespring software, by Agilent Technologies (1 mentions)
Matlab, by MathWorks (1 mentions)
Stata version 12, by StataCorp (1 mentions)
Quantity one, by Bio-Rad (1 mentions)
Spss version 21.0 for windows, by IBM (1 mentions)
Amplex red hydrogen peroxide peroxidase assay kit, by Thermo Fisher Scientific (1 mentions)
Spss software version 22, by IBM (1 mentions)
Spss 17, by IBM (1 mentions)
Spectramax m5, by Molecular Devices (1 mentions)
Spss statistics 21, by IBM (1 mentions)
140 protocols using prism 5.0 for windows
1
Statistical Analysis of Data
2
Statistical Methods for Biological Data
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Correlations of two-sided linear dependencies between diverse variables were determined using the Pearson correlation coefficient. The Pearson correlation coefficient is based on a 95% reliability interval, with p < 0.05 considered statistically significant. Statistical analysis was performed using the software GraphPad Prism 5.0 for Windows (GraphPad Software, San Diego, CA, USA). The data obtained from molecular, immunohistochemical, clinical, haematological and histological analyses were evaluated by one-way ANOVA, followed by the Tukey post hoc test using multiple comparison. Significant differences between experimental animals were tested by analysis of variance and non-paired Student t-test. Results are expressed as means ± SD. Differences between results were considered significant at least at p < 0.05. ANOSIM (analysis of similarity) statistical test was used to calculate the difference between the community composition of samples of the FMT and DSS-FMT groups. The Mann–Whitney non-parametric test was used to calculate the significance of alpha diversity indices between the groups FMT and DSS- FMT and to calculate the significance of the relative abundance of selected bacterial taxa between FMT and DSS-FMT using the vegan R package and GraphPad software.
3
Statistical Analysis of Experimental Data
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All statistical analyses were performed using GraphPad Prism 5.0 for Windows (GraphPad Software Inc., San Diego, CA). Normal distribution and homogeneity of variance of the data were tested using Shapiro–Wilk and F tests, respectively. One-way ANOVA followed by Tukey's post hoc tests and Student's t-tests were performed to determine differences between treatments. Two-way ANOVA and Bonferroni's post hoc tests were used when appropriate. All values in figures and tables are presented as mean ± SEM. Statistical significance was set at p < 0.05.
4
Triplicate Experiments with Statistical Analysis
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All experiments were performed at least three times in triplicate, and the results were displayed as mean ± SEM. Statistical analyses were performed using GraphPad Prism 5.0 for Windows (GraphPad Software, San Diego, CA, USA). In the statistical analysis, a one-way ANOVA was used with Dunnett’s multiple-comparison test.
5
Investigating Neuronal Excitability Dynamics
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Data shown are means ± SEM; t test was used for determination of statistical difference between groups. Analyses were performed using GraphPad Prism 5.0 for Windows (GraphPad Software, San Diego, CA). P < 0.05 was considered statistically significant.
6
Immunological Analyses of Experimental Protocol
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Statistical analyses were performed using Graph-Pad Prism 5.0 for Windows (Graphpad Software Inc 2007, San Diego, USA) as well as SPSS version 18. Data were expressed as median. Each group (G1 and G2) were compared with control PBS group (G3). In some cases, G1 and G2 were compared similarly. Non-parametric analysis were used for all tests including humoral, cellular immune responses, DTH responses and parasite load since they were not normally distributed. Mann—Whitney test and Fishers exact test were also used for the comparison of different parameters between groups. The correlation between the IFN-γ and IgG2 production at 14 and 17 months after challenge was calculated using Spearman correlation method for each group (G1, G2 and G3). The p value <0.05 was considered significant.
7
Determination of LC3B-Peptide Binding Affinity
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Binding experiments were done in a black 384-microwell plate. The CF-labelled peptides and purified LC3B protein were dissolved in a HEPES buffer. Each CF-labelled peptide in a volume of 25 µL at a concentration of 2.5 µM was combined with 25 µL of LC3B at a concentration range from 0.2–330 µM. The fluorescent polarization was measured with the excitation and emission wavelengths of 535 nm and 585 nm, respectively. The obtained polarization data were corrected by subtraction of the peptide alone (blank). The dissociation constant (Kd) was quantified using a nonlinear curve fitting the site-specific binding on GraphPad Prism 5.0 for Windows (GraphPad Software, La Jolla, CA, USA). Experiments were performed in three independent replications.
8
Statistical Analysis of Research Data
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Statistical analyses were performed using GraphPad Prism 5.0 for Windows (GraphPad Software, Inc., La Jolla,CA, USA). Normal distribution of the data was tested with the Kolmogorov–Smirnov test. While parametric data were assessed for statistical correlation using Pearson’s correlation, nonparametric data were assessed using Spearman’s rho. A Fisher’s r-to-z transformation test was performed to test for potential differences between correlations. To compare the study groups in terms of continuous variables, the unpaired two-sided t-test and ANOVA were used with the normally distributed data while the Mann−Whitney U test and Kruskal-Wallis test were used with the non-normally distributed data. P values of <0.05 were considered as statistically significant.
9
Statistical Analysis of Experimental Data
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All statistical analyses were performed using GraphPad Prism 5.0 for Windows (GraphPad Software Inc., San Diego, CA, United States). Two-way ANOVA and Bonferroni’s post hoc tests when used when appropriate. All values are presented as mean ± SEM. Statistical significance was set at p < 0.05.
10
Comparative Efficacy of rBCG Variants
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Statistical significance was determined using one-way ANOVA with Kruskal-Wallis tests and Dunnett tests of GraphPad Prism 5.0 for Windows (GraphPad Software, Inc., La Jolla, CA, USA). PBS group was regarded as negative control. The remaining 4 groups were compared with the rBCG group. P<0.05 was considered to indicate a statistically significant difference.
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