Gold 3 chloride trihydrate haucl4 3h2o

Synthesis of AuNPs was conducted using wet chemistry methods as previously described [34 (link)]. Briefly, 1 mL of 25 mM HAuCl₄ was rapidly added to 150 mL of a boiling aqueous solution of sodium citrate 2.2 mM. The formation of AuNPs (~10 nm) occurred in a few minutes, and was followed by growth-inducing steps, consisting of the addition of HAuCl₄ until reaching the desired NP size. Au NPs of 12 nm diameter were used in this study. Sodium citrate tribasic dihydrate (≥99% purity), gold (III) chloride trihydrate HAuCl₄·3H₂O (99.9% purity) were from Sigma-Aldrich, Inc. (St. Louis, MO, USA). All reagents were used as received without further purification, and all glass material was sterilized and dehydrogenated in an oven prior to use. Pyrogen-free milli-Q water was used in the preparation of all solutions. AuNPs were purified by centrifugation to discard byproducts and contaminants, then NPs were resuspended in a solution of 2.2 mM sodium citrate (final pH = 6.4) and stored at 4 °C in the dark.

Gold(III) chloride trihydrate (HAuCl₄·3H₂O) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The 3T3-L1 preadipocytes were obtained from the American Type Culture Collection (Manassas, VA, USA). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Welgene (Gyeongsan-si, South Korea). In addition, deionized (DI) water was used for the green synthesis of AuNPs.

The substrates used in this experiment are the microscope glass slides purchased from Technologist Choice, Bio Nuclear diagnostics Inc., with a glass transition temperature, T_g = 820°C. Substrates are cut to the size of 37 mm x 12.5mm x 1 mm. Gold (III) chloride trihydrate (HAuCl₄.3H₂O) and sodium citrate were purchased from Sigma Aldrich. De-ionized (DI) water with a resistivity of 18MΩ, used in all the experiments was obtained from the NANO pure ultrapure water system (Barnstead). 11-mercaptoundecanoic acid in ethanol (Nano Thinks Acid 11), N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) and N-Hydroxy succinimide (NHS), and phosphate buffered saline (PBS) was obtained from Sigma Aldrich, Canada. PBS tablets were dissolved in DI water at 0.1M concentration (pH = 7.2). Streptavidin was purchased from IBA GmBH. Biotin-PEG-Vn96 was purchased from New England Peptide. MCF7 exosomes were purified from MCF7 cells conditioned media in a bioreactor, using ultracentrifugation, and resuspended in fetal bovine serum free cell-culture media and the exosome concentration was determined by using NTA [24 (link)].