Water bath

Manufactured by Avantor

Sourced in United States

A water bath is a laboratory instrument used to maintain a consistent temperature in a controlled environment. It typically consists of an insulated container filled with water that can be heated or cooled to a specific temperature. The water bath provides a stable and uniform temperature for various applications, such as incubation, thawing, and temperature-sensitive reactions.

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Lab products found in correlation

2d quant assay, by GE Healthcare (1 mentions) Proteinase k, by Merck Group (1 mentions) Catalase, by MP Biomedicals (1 mentions) Thermo scientific varioskan lux, by Thermo Fisher Scientific (1 mentions) 1260 infinity, by Agilent Technologies (1 mentions) Sl16r, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Ultrasonic water bath (5 citations) Circulating water-bath (4 citations) Water bath sonicator (2 citations) Reciprocating water bath (2 citations)

6 protocols using water bath

Optimized Wheat Grain Protein Extraction

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Individual wheat grains (cv Hereward) were crushed between filter paper using pliers, and transferred to a clean Eppendorf. To three crushed grains (per extraction) 250 μL of extraction buffer was added.
Rapigest™ was included in all extracts at 0.2% (w/v) in order to improve protease digestion. All extractions were carried out for 15 min with sonication in a water bath heated to 60°C (VWR, Leicestershire, UK), vortexing every 5 min. Extracts were then centrifuged at 10,000 × g for 10 min, the supernatant collected and transferred to a clean microcentrifuge tube. Three biological replicates were extracted with each buffer. Protein concentration of the biological replicates for each extraction was initially determined using 2D Quant Assay™ (GE Healthcare, Buckinghamshire UK) as per the manufacturer's instructions using bovine serum albumin as a standard. The protein extraction rate was calculated as a percentage of the total grain protein determined with a Kjeldhal analysis using the Dumas combustion method for each extraction.

Bromilow S.N., Gethings L.A., Langridge J.I., Shewry P.R., Buckley M., Bromley M.J, & Mills E.N. (2017). Comprehensive Proteomic Profiling of Wheat Gluten Using a Combination of Data-Independent and Data-Dependent Acquisition. Frontiers in Plant Science, 7, 2020.

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Exposure of Cells to Multi-Walled Carbon Nanotubes

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Immediately prior to cell exposures, MWNTs were suspended in serum-free DCCM-1 at a concentration of 1 mg/mL, vortexed for 1 minute and then sonicated in a water bath (42 kHz, 135 W, VWR International, USA) for 10 minutes. This stock solution was diluted in DCCM-1 to the required doses (0 – 50 µg/mL) and sonicated briefly prior to cell exposure. The cells were exposed to MWNTs with required doses (0 – 50 µg/mL) for 24 hours. All exposures were performed in triplicate.

Chen S., Hu S., Smith E.F., Ruenraroengsak P., Thorley A.J., Menzel R., Goode A.E., Ryan M.P., Tetley T.D., Porter A.E, & Shaffer M.S. (2014). Aqueous cationic, anionic and non-ionic multi-walled carbon nanotubes, functionalised with minimal framework damage, for biomedical application. Biomaterials, 35(17), 4729-4738.

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Zoospore Exudate Characterization

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Zoospore exudate was examined for its activity to various factors, including multiple temperature and enzymes. To test the effect of temperature, 10 ml ZE was incubated in a 15-ml tube, which was placed in water bath (VWR Scientific Inc., Radnor, PA, United States) at temperatures of 40, 60, 80, and 100°C for 20 min, or in an autoclave (Amsco Lab 250 sterilizer, Steris Inc., Mentor, OH, United States) at 121°C for 15 min. Proteinase K (Sigma-Aldrich, Inc., St. Louis, MO, United States, 37°C, pH 7.5), and catalase (MP Biomedicals, 25°C, pH 7.0) were tested for their effect on activity by incubating ZE with 10 mg/ml of each enzyme for 2 h at the optimal temperature according to the manufacturer’s instruction. Zoospore germination and tuber infection were tested using treated ZE as described above. Enzymes and solvents only were tested at the same time as negative controls. Four replicates were set for each treatment, and the experiments were conducted twice.

Jiang H., Hwang H.W., Ge T., Cole B., Perkins B, & Hao J. (2019). Leucine Regulates Zoosporic Germination and Infection by Phytophthora erythroseptica. Frontiers in Microbiology, 10, 131.

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Multiplex RNAscope for Cardiac Markers

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In situ hybridization was performed using formalin-fixed paraffin embedded tissue samples and the RNAscope Multiplex Detection KIT V2 (RNAscope, cat. no. 323100) and RNAscope 4-Plex Ancillary Kit (RNAscope, cat. no. 323120) following the manufacturer’s protocol with minor modifications. The antigen retrieval was performed for 30 min at 99 °C in a water bath (VWR). Tissue pretreatment and washing was performed as suggested by the RNAscope staining protocol. The following probes were used for the RNAscope assay: Hs-CD163 cat. no. 417061-C1, Hs-CCR2 cat. no. 438221-C1, Hs-ANKRD1 cat. no. 524241-C1, Hs-POSTN cat. no. 575941-C1, Hs-Col15a1 cat. no. 484001-C2, Hs-Col1a1 cat. no. 401891-C2, Hs-PECAM1-O2 cat. no. 487381-C2, Hs-NPPB cat. no. 448511-C2, Hs-TREM2 cat. no. 420491-C2, Hs-SPP1 cat. no. 420101-C2, Hs-NPR3 cat. no. 431241-C3, Hs-POSTN cat. no. 409181-C3, Hs-SCARA5 cat. no. 574781-C3, Hs-TNNT2 cat. no. 518991-C3, Hs-SPP1 cat. no. 420101-C4 and Hs-NFE2L1 cat. no. 53850.

Kuppe C., Ramirez Flores R.O., Li Z., Hayat S., Levinson R.T., Liao X., Hannani M.T., Tanevski J., Wünnemann F., Nagai J.S., Halder M., Schumacher D., Menzel S., Schäfer G., Hoeft K., Cheng M., Ziegler S., Zhang X., Peisker F., Kaesler N., Saritas T., Xu Y., Kassner A., Gummert J., Morshuis M., Amrute J., Veltrop R.J., Boor P., Klingel K., Van Laake L.W., Vink A., Hoogenboezem R.M., Bindels E.M., Schurgers L., Sattler S., Schapiro D., Schneider R.K., Lavine K., Milting H., Costa I.G., Saez-Rodriguez J, & Kramann R. (2022). Spatial multi-omic map of human myocardial infarction. Nature, 608(7924), 766-777.

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Thermal Stability Profiling of Proteins

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Protein samples were diluted in buffer A to 0.2 mg/mL and each sample (100 µL in triplicate) was heated for 10 min in a water bath (VWR, PA, USA) at 25, 30, 37, 40, 45, 50, 52, 55, 57, 60, 65, 67, 70, 75, and 85 °C and placed on ice prior to measurement. The LmTIM_E65Q was subjected to higher temperatures due to its known higher thermostability (25, 50, 55, 60, 65, 70, 75, 78, 80, 83, 86, 90, 95, and 99 °C. The samples were analyzed in white closed-bottom microtiter plates (Hamilton, Reno (NV), USA) with a Thermo Scientific Varioskan LUX (ThermoFisher Scientific, Waltham (MA), USA). IgG, BSA, OVA, and F_ab samples were excited at 295 nm and the emission spectrum were recorded between 314–550 nm, while LmTIM_E65Q was excited at 280 nm and the emission was recorded at 290–550 nm.

Itkonen J., Ghemtio L., Pellegrino D., Jokela (née Heinonen) P.J., Xhaard H, & Casteleijn M.G. (2022). Analysis of Biologics Molecular Descriptors towards Predictive Modelling for Protein Drug Development Using Time-Gated Raman Spectroscopy. Pharmaceutics, 14(8), 1639.

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Glucosinolates and Lutein Extraction from Kale

Cited 1 time

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Extraction of glucosinolates and lutein from kale sprouts powder was carried out in a single process based on the protocol published by Moreira-Rodríguez et al. [32 (link)]. Briefly, kale samples (0.2 g) were mixed with 10 mL of ethanol/water (70:30, v/v), previously heated for 10 min at 70 °C to inactivation of myrosinase, followed by the addition of 50 μL of sinigrin 3 mM as internal standard (I.S.).
Samples were immersed in a water bath (VWR, Radnor, PA, USA) at 70 °C and vortexed every 5 min for 30 min to inactivate myrosinase. Then, the extracts were left to cool at 25 °C and centrifuged (18,000× g, 10 min, 4 °C) (SL16R, Thermo Scientific, GER) to recover the supernatant. The supernatant obtained was used for the identification and quantification of individual glucosinolates, lutein, and phenolic compounds by HPLC-DAD (1260 Infinity, Agilent Technology, Santa Clara, CA, USA).

Ortega-Hernández E., Antunes-Ricardo M., Cisneros-Zevallos L, & Jacobo-Velázquez D.A. (2022). Selenium, Sulfur, and Methyl Jasmonate Treatments Improve the Accumulation of Lutein and Glucosinolates in Kale Sprouts. Plants, 11(9), 1271.

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