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Anti cd14

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Anti-CD14 is a laboratory reagent used in immunohistochemistry (IHC) and flow cytometry applications. It binds to the CD14 antigen, which is expressed on the surface of monocytes, macrophages, and other cell types. This reagent can be used to identify and characterize these cell populations in research and clinical settings.

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Lab products found in correlation

Anti smad7, by Santa Cruz Biotechnology (2 mentions) Opal 7 color automation ihc kit, by Akoya Biosciences (1 mentions) Anti cd19, by Abcam (1 mentions) Anti pan cytokeratin, by Abcam (1 mentions) Fluoromount g mounting medium, by Southern Biotech (1 mentions) Vectra 3 automated quantitative pathology imaging system, by PerkinElmer (1 mentions)

3 protocols using anti cd14

1

Multiplex IHC Analysis of FFPE Samples

Cited 1 time
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Multiplex immunohistochemistry of patient FFPE samples was done in sequential staining cycles using the Opal 7-color Automation IHC Kit (Akoya Biosciences, NEL801001KT) on the BOND RX IHC & ISH Research Platform (Leica Biosystems), which was optimized and performed as described before (33 (link), 34 (link)). The multiplex panel consisted of 1:200 anti-CD14 (Cell Marque, 114R-16) with Opal620, 1:200 anti-CD19 (Abcam, ab134114) with Opal690, 1:150 anti-BDCA2 (Dendritics, DDX0043) with Opal540, 1:100 anti-CD1c (Thermo Fisher Scientific, TA505411) with Opal520, 1:100 XCR1 (Cell Signaling Technologies, 44665S) with Opal570 and 1:1500 anti-pan cytokeratin (Abcam, ab86734) with Opal650. Slides were counterstained with DAPI for 5 minutes and enclosed in Fluoromount-G mounting medium (SouthernBiotech, 0100-01). Whole tissue slides were imaged using the microscope Vectra 3 Automated Quantitative Pathology Imaging System (Version 3.0.4, PerkinElmer Inc.). For comparison to the co-cultures with PDTOs, only DAPI, CD1c, and Pan cytokeratin are shown.
Subtil B., Iyer K.K., Poel D., Bakkerus L., Gorris M.A., Escalona J.C., van den Dries K., Cambi A., Verheul H.M., de Vries I.J, & Tauriello D.V. (2023). Dendritic cell phenotype and function in a 3D co-culture model of patient-derived metastatic colorectal cancer organoids. Frontiers in Immunology, 14, 1105244.
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2

Immunofluorescence Staining of Formalin-Fixed Tissue

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We used our previously-described immunofluorescence protocol 14 (link) to stain serial sections of formalin-fixed, paraffin-embedded tissue. Slides were incubated with either rabbit polyclonal anti–Smad7 (Santa Cruz Biotechnology), mouse monoclonal anti-CD14 (Cell Marque, Rocklin, California), or irrelevant isotype-matched antibody overnight at 4°C. Sections then were washed, incubated with anti-mouse or anti-rabbit secondary antibodies, washed, stained with DAPI, and visualized by confocal microscopy.
Dennis E.A., Smythies L.E., Grabski R., Li M., Ballestas M.E., Shimamura M., Sun J.J., Grams J., Stahl R., Niederweis M.E., Britt W.J, & Smith P.D. (2018). Cytomegalovirus Promotes Intestinal Macrophage-mediated Mucosal Inflammation through Induction of Smad7. Mucosal immunology, 11(6), 1694-1704.
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3

Immunofluorescence Staining of Formalin-Fixed Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
We used our previously-described immunofluorescence protocol 14 (link) to stain serial sections of formalin-fixed, paraffin-embedded tissue. Slides were incubated with either rabbit polyclonal anti–Smad7 (Santa Cruz Biotechnology), mouse monoclonal anti-CD14 (Cell Marque, Rocklin, California), or irrelevant isotype-matched antibody overnight at 4°C. Sections then were washed, incubated with anti-mouse or anti-rabbit secondary antibodies, washed, stained with DAPI, and visualized by confocal microscopy.
Dennis E.A., Smythies L.E., Grabski R., Li M., Ballestas M.E., Shimamura M., Sun J.J., Grams J., Stahl R., Niederweis M.E., Britt W.J, & Smith P.D. (2018). Cytomegalovirus Promotes Intestinal Macrophage-mediated Mucosal Inflammation through Induction of Smad7. Mucosal immunology, 11(6), 1694-1704.
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