Dichloromethane dcm

2-[2-[2-Cholro-3-[[1,3-dihydro-1,1-dimethyl-3-(4-sulfobutyl)-2H-benzo[e]indol-2-ylidene]-ethylidene-1-cyclohexen-1-yl]-ethenyl]-1,1-dimethyl-3-(4-sulfobutyl)-1H-benzo [e]indolium hydroxide inner salt (NaIR820), Nile Blue A sulfate (NBA₂SO₄), deuterated dimethyl sulfoxide (d-DMSO), and Wilmad 5 mm NMR tubes were purchased from Sigma-Aldrich. Ethanol and dichloromethane (DCM) were purchased from VWR. Alumina cells for thermal stability analysis were purchased from Perkin Elmer. Distilled deionized water of 18.2 MΩ cm was obtained from ultrapure distilled water purifier (ELGA).

Unless otherwise indicated, all chemicals used for preparation of the scaffold and cell sheet decellularization were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dichloromethane (DCM) was purchased from VWR Chemicals (Radnor, PA, USA).
All cells used were purchased from ATCC, except that the mesenchymal stem cells (MSC) were purchased from Innoprot (Bizkaia, Spain), and the human chondrocyte cells were purchased from Cell Application INC (San Diego, CA, USA). Culture reagents, including fetal bovine serum (FBS), penicillin–streptomycin, and trypsin, were purchased from Life Technologies (Carlsbad, CA, USA). The culture media were purchased from the following sources: rat mesenchymal basal medium and its growth supplement, Cell Application Inc.; Eagle’s minimum essential medium, ATCC; Dulbecco’s modified Eagle medium/nutrient mixture F-12, Gibco (Billings, MT, USA); Dulbecco’s modified Eagle medium, Gibco.
The analytical reagents were purchased from the following sources: CellTiter Blue cell viability assay, Promega (Madison, WI, USA); Quant-iT™ PicoGreen^® dsDNA, Invitrogen (Waltham, MA, USA); BCA assay kit, Thermo Fisher Scientific (Waltham, MA, USA).

PCL (Mw = 80,000), PEP essential oil (purified by triple-distillation), standard menthol, and fetal bovine serum (FBS) were purchased from Sigma-Aldrich (Darmstadt, Germany). Glacial acetic acid (GAA, VWR, Darmstadt, Germany) was used as a solvent. Phosphate-buffered saline (PBS, biotech grade, pH 7.4) and dichloromethane (DCM) were obtained from VWR (Darmstadt, Germany). The microorganism strains of S. aureus (ATCC25923), and E. coli (ATCC25922) were used in our laboratory. Luria-Bertani (LB) agar and lysogeny broth (LB) medium were supplied by Carl Roth GmbH (Karlsruhe, Germany). Dulbecco's modified Eagle‘s medium (DMEM), penicillin/streptomycin (PS), and trypsin/EDTA were purchased from Thermo Scientific (Schwerte, Germany). NHDF cell line was obtained from Translation Research Center (TRC), Erlangen. All reagents and solvents were of analytical grade.