Gp91phox nox2

Manufactured by Santa Cruz Biotechnology

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Gp91phox/NOX2 is a protein subunit of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex. It serves as the catalytic core of the enzyme, responsible for the generation of superoxide radicals.

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Gp91phox (26 citations)

2 protocols using gp91phox nox2

Investigating Oxidative Stress Signaling

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Primary antibodies against gp91phox/NOX2, p47phox, acetylated α-tubulin, p65, IkB, c-fos, and c-jun were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Total-ERK, total-p38, total-JNK, phosphor-ERK, phosphor-p38, phosphor-JNK, β-actin, and HDAC6 were purchased from Cell Signaling Technology (Beverly, MA, U.S.A.). Hindsiipropane B was synthesized from commercially available starting materials with the Wittig-Horner reaction, Claisen-Schmidt condensation and hydrogenation as key steps (14 (link)). 10 mM stock solution of hindsiipropane B was prepared in dimethyl sulfoxide. Oligonucleotide primers (HDAC6, CCL2, CXCL8, CXCL10, Nox2, p22phox, p47phox, and β-actin) (Bioneer, Seoul, Korea) were obtained from commercially.

Jo H., Jang H.Y., Youn G.S., Kim D., Lee C.Y., Jang J.H., Choi S.Y., Jun J.G, & Park J. (2018). Hindsiipropane B alleviates HIV-1 Tat-induced inflammatory responses by suppressing HDAC6-NADPH oxidase-ROS axis in astrocytes. BMB Reports, 51(8), 394-399.

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Doxorubicin-Induced Mitochondrial Dysfunction

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Doxorubicin, neferine, DCF-DA, FURA 2/AM, JC-1, DAPI, rotenone, lysis buffer (CelLytic™), U0126 ERK1/2 and SB203580 p38 inhibitor were purchased from Sigma (Bangalore, India). Dulbecco’s Modified Eagle’s Medium (DMEM), Fetal Bovine Serum (FBS) and antibiotic solutions were obtained from HiMedia Laboratories (Mumbai, India). DiOC6 (3,3′-dihexyloxacarbocyanine iodide) and CCCP [carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone] were obtained from Calbiochem, (San Diego, CA, USA). PVDF membranes were obtained from Whatman (Clifton, NJ, USA). Protein marker (PageRuler™) and all other chemical used for SDS-PAGE were obtained from Thermo Scientific (Bangalore, India). Primary monoclonal antibodies for p22phox (sc-20781), p47phox (sc-14015), gp91phox/Nox2 (sc-5827), p-ERK1/2 (sc-7383), p-p38 (sc-7973), NfκB (sc-109), COX2 (sc-1745), TNF-α (sc-1350), Bcl-2 (sc-7382), Bax (sc-526), p-Bad (sc-101641), cytochrome C (sc-13560), caspase-9 (sc-8355), β-actin (sc-47778), α-tubulin (sc-5286), cyclin D1 (sc-246), GAPDH (sc-25778), HDAC1 (sc-7872) and HRP conjugated secondary antibodies raised against mouse, rabbit and goat were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibody against caspase-3 (#9665 s) was purchased from Cell Signalling Technology (Danver, MA 01923, USA).

Priya L.B., Baskaran R., Huang C.Y, & Padma V.V. (2017). Neferine ameliorates cardiomyoblast apoptosis induced by doxorubicin: possible role in modulating NADPH oxidase/ROS-mediated NFκB redox signaling cascade. Scientific Reports, 7, 12283.

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