Cd11b

cMSCs were isolated from sham-operated and HF mice (8 weeks’ post-MI) using primary explant cell outgrowth culture. Cell outgrowths from minced myocardial tissue were cultured in cMSC media (Dulbecco’s modified Eagle medium [DMEM]/Ham’s F-12 culture medium [Thermo Fisher Scientific–HyClone] supplemented with 0.2 mM l-glutathione, 10 ng/mL leukemia inhibitory factor, 10 ng/mL basic fibroblast growth factor, 10% fetal bovine serum, and 1% penicillin-streptomycin). Lineage-positive (Lin⁺) cells (CD5⁺, CD45R⁺, Cd11b⁺, Gr-1⁺, TER-119⁺; Miltenyi Biotec, #130-092-613), endothelial cells (CD31⁺; BD, #553372), and fibroblasts (discoidin domain receptor 2 positive [DDR2⁺]; LSBio, #LS-C255961) were depleted from explant cultures by using antibody-conjugated magnetic microbeads. After depletion, Lin⁻CD31⁻DDR2⁻ cells were minimally expanded and further sorted by using a specific Sca1 antibody (eBioscience, #25-5981-81) and consequently enriched for Sca1⁺ cells. Lin^–CD31^–DDR2^–Sca1⁺ cells were minimally expanded and used at cell passages 3 to 5 for all experiments. cMSCs were evaluated for their ability to differentiate into adipogenic, osteogenic, and chondrogenic lineages using standard differentiation protocols.⁹ (link) Lineage-specific gene expression was used to confirm successful differentiation as described.⁹ (link)^,28 (link)

Colonic LPMCs were separated by MACS. LPMCs with specific CD antibodies were magnetically labeled with their respective magnetic beads (CD11c, #130-108-338; CD11b, #130-049-601; CD4, #130-049-201; Miltenyi Biotec). The cell suspension was loaded onto a MACS^® LS Column (#130-042-401; Miltenyi Biotec), which was placed in the magnetic field of a MACS Separator (#130-042-301; Miltenyi Biotec). The MACS Separation Buffer contained a final BSA (Miltenyi Biotec) concentration of 0.5%. After cells were separated, they were analyzed for gene expression.