Dp25 imaging system

Manufactured by Olympus

Sourced in United States

The DP25 imaging system is a digital camera designed for microscopy applications. It features a 5.0-megapixel CMOS sensor and can capture high-resolution images. The DP25 is capable of live image preview and provides connectivity options for data transfer and image analysis.

Automatically generated - may contain errors

Lab products found in correlation

Bx40 microscope, by Olympus (7 mentions) Positively charged slides, by Avantor (6 mentions) Eosin y, by Avantor (6 mentions) Hematoxylin qs, by Vector Laboratories (6 mentions) Vectamount mounting media, by Vector Laboratories (3 mentions) Cytoseal xyl mounting media, by Thermo Fisher Scientific (3 mentions) Fluorometric activity assay, by Merck Group (2 mentions) Total bilirubin elisa, by Cusabio (2 mentions) Catalyst one serum chemistry analyzer, by IDEXX (2 mentions)

7 protocols using dp25 imaging system

Histological and Biochemical Analysis of Liver in AOM-Treated Mice

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Paraffin-embedded livers from vehicle and AOM-treated mice were sectioned into 3 µm sections and mounted onto positively charged slides (VWR, Radnor, PA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories, Burlingame, CA) for one minute followed by staining for one minute with eosin Y (Amresco, Solon, OH) and rinsed in 95% ethanol. The slides were then dipped into 100% ethanol and subsequently through 2 xylene washes. Coverslips were mounted onto the slides using Vectamount mounting media (Vector Laboratories). The slides were viewed and imaged using an Olympus BX40 microscope with an Olympus DP25 imaging system (Olympus, Center Valley, PA).
Serum ALT and bilirubin were assessed using commercially available kits. Alanine aminotransferase measurement was performed using a fluorometric activity assay (Sigma-Aldrich, St. Louis, MO). Total bilirubin was assayed using a total bilirubin ELISA (CusaBio, Wuha, China). All assays and subsequent analyses were performed according to the manufacturer’s instructions.

McMillin M., Frampton G., Tobin R., Dusio G., Smith J., Shin H., Newell-Rogers M.K., Grant S, & DeMorrow S. (2015). TGR5 signaling reduces neuroinflammation during hepatic encephalopathy. Journal of neurochemistry, 135(3), 565-576.

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Histological Analysis of Paraffin-Embedded Liver Sections

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Paraffin-embedded livers were cut into 4-μm sections and mounted onto positively charged slides (VWR, Radnor, PA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories, Burlingame, CA) followed by staining with eosin Y (Amresco, Solon, OH) and rinsed in 95% ethanol. The slides were then dipped into 100% ethanol and subsequently through two xylene washes. Coverslips were mounted onto the slides using CytoSeal XYL mounting media (ThermoFisher). The slides were viewed and imaged using an Olympus BX40 microscope with an Olympus DP25 imaging system (Olympus, Center Valley, PA). All images are taken as × 200 magnification.
Liver function was assessed by measuring serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels using a Catalyst One serum chemistry analyzer from IDEXX Laboratories, Inc. (Westbrook, MA).

McMillin M., Grant S., Frampton G., Petrescu A.D., Williams E., Jefferson B., Thomas A., Brahmaroutu A, & DeMorrow S. (2019). Elevated circulating TGFβ1 during acute liver failure activates TGFβR2 on cortical neurons and exacerbates neuroinflammation and hepatic encephalopathy in mice. Journal of Neuroinflammation, 16, 69.

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Histological Assessment of Liver Damage

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Liver damage was assessed by H&E staining according to previously published protocols.⁷ (link) Paraffin-embedded livers were cut into 4-μm sections and mounted onto positively charged slides (VWR, Radnor, PA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories) for 1 minute followed by staining for 1 minute with eosin Y (Amresco, Solon, OH) and rinsed in 95% ethanol. The slides then were dipped into 100% ethanol and subsequently through 2 xylene washes. Coverslips were mounted onto the slides using CytoSeal XYL mounting media (Thermo Fisher Scientific, Waltham, MA). The slides were viewed and imaged using an Olympus BX40 microscope with an Olympus DP25 imaging system (Center Valley, PA).
Liver function was assessed by measuring plasma alanine aminotransferase and aspartate aminotransferase using the IDEXX Catalyst One machine from IDEXX Laboratories, Inc.

McMillin M., Grant S., Frampton G., Petrescu A.D., Kain J., Williams E., Haines R., Canady L, & DeMorrow S. (2018). FXR-Mediated Cortical Cholesterol Accumulation Contributes to the Pathogenesis of Type A Hepatic Encephalopathy. Cellular and Molecular Gastroenterology and Hepatology, 6(1), 47-63.

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Histological Liver Analysis and Serum Biomarkers

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Paraffin-embedded livers were cut into 3-μm sections and mounted onto positively charged slides (VWR, Radnor, PA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories, Burlingame, CA) for 1 min, followed by staining for 1 min with eosin Y (Amresco, Solon, OH), and rinsed in 95 % ethanol. The slides were then dipped into 100 % ethanol and subsequently through two xylene washes. Coverslips were mounted onto the slides using Vectamount mounting media (Vector Laboratories). The slides were viewed and imaged using an Olympus BX40 microscope with an Olympus DP25 imaging system (Olympus, Center Valley, PA).
Serum alanine aminotransferasase (ALT) and bilirubin were assessed using commercially available kits. Alanine aminotransferase measurement was performed using a fluorimetric activity assay. Total bilirubin was assayed using a total bilirubin ELISA. All assays and subsequent analyses were performed according to the instructions provided by the manufacturers.

McMillin M., Grant S., Frampton G., Andry S., Brown A, & DeMorrow S. (2016). Fractalkine suppression during hepatic encephalopathy promotes neuroinflammation in mice. Journal of Neuroinflammation, 13(1), 198.

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Liver Histopathology and Serum Biomarkers

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Liver tissue specimens procured from the left lateral hepatic lobe were fixed with 10% neutral formaldehyde solution and embedded with paraffin. Then, the tissue specimens were sectioned into 3 μm sections and stained with hematoxylin and eosin (HE) using standard procedures. The slides were viewed and imaged using an Olympus BX40 microscope with Olympus DP25 imaging system (Olympus, USA). Serum alanine aminotransferase (ALT) and bilirubin were determined using commercially available kits (Nanjing Jiancheng, China) according to manufacturers’ instructions.

Zhang L., Tan J., Jiang X., Qian W., Yang T., Sun X., Chen Z, & Zhu Q. (2017). Neuron-derived CCL2 contributes to microglia activation and neurological decline in hepatic encephalopathy. Biological Research, 50, 26.

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Histochemical and Biochemical Analysis of Liver Function

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Paraffin-embedded livers were sectioned into 3 μm sections and mounted onto positively charged slides (VWR, Radnor, PA, USA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories, Burlingame, CA, USA) for 1 min followed by staining for 1 min with eosin Y (Amresco, Solon, OH, USA) and rinsed in 95% ethanol. The slides were then dipped into 100% ethanol and subsequently through two xylene washes. Coverslips were mounted onto the slides using Vectamount mounting media (Vector Laboratories). The slides were viewed and imaged using an Olympus BX40 microscope with an Olympus DP25 imaging system (Olympus, Center Valley, PA, USA).
Serum alanine aminotransferase (ALT) and bilirubin were assessed using commercially available kits. ALT measurement was performed using a fluorometric activity assay (Sigma-Aldrich). Total bilirubin was assayed using a total bilirubin ELISA (CusaBio, Wuha, China). All assays and subsequent analyses were performed according to manufacturers’ instructions.

McMillin M., Frampton G., Thompson M., Galindo C., Standeford H., Whittington E., Alpini G, & DeMorrow S. (2014). Neuronal CCL2 is upregulated during hepatic encephalopathy and contributes to microglia activation and neurological decline. Journal of Neuroinflammation, 11, 121.

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Quantification of Liver Necrosis and Function

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Paraffin-embedded livers were cut into 4 μm sections and mounted onto positively-charged slides (VWR, Radnor, PA). Slides were deparaffinized and stained with Hematoxylin QS (Vector Laboratories, Burlingame, CA) followed by staining with Eosin Y (Amresco, Solon, OH) and rinsed in 95% ethanol. The slides were then dipped into 100% ethanol and subsequently through 2 xylene washes. Coverslips were mounted onto the slides using CytoSeal XYL mounting media (ThermoFisher Scientific). The slides were viewed and imaged using an Olympus BX40 microscope with a DP25 imaging system (Olympus, Center Valley, PA). Percentage area of necrosis was calculated using 20× field views, drawing areas devoid of hepatocyte nuclei and calculating area using ImageJ software (National Institutes of Health, Bethesda, MD). In each mouse, the necrosis from 5 to 10 fields were quantified and averaged together to determine necrotic area.
Liver function was assessed by measuring serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels using a Catalyst One serum chemistry analyzer from IDEXX Laboratories, Inc. (Westbrook, MA). For control groups, serum was diluted 1:2 in deionized H₂0 and for APAP-treated groups, serum was diluted 1:9 in deionized H₂0 prior to running on the analyzer.

Frampton G., Reddy P., Jefferson B., Ali M., Khan D, & McMillin M. (2020). Inhibition of thrombospondin-1 reduces glutathione activity and worsens acute liver injury during acetaminophen hepatotoxicity in mice. Toxicology and applied pharmacology, 409, 115323.

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