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2 protocols using orthophosphoric acid

1

Synthesis of Graphene Oxide Nanoparticles

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Graphite Flakes (99%, Sigma-Aldrich), Sodium nitrate (98%), Potassium permanganate (99%), Hydrogen peroxide (30% wt), Sulphuric acid (98%), Orthophosphoric acid (35%) were all obtained from Himedia. The synthesis of Graphene Oxide nanoparticles were done according to the method described in Marcano et al. [29 (link)].
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2

Biopolymer-based Composite Hydrogel

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Chitosan (medium molecular weight ~1,90,000–3,10,000 daltons, <200 mPa.s in viscosity, deacetylation degree ≥75%), gelatin (50,000–1,00,000 daltons in molecular weight and 5.10–5.80 mPa.s in viscosity), sodium alginate (216.12 g/mol in molecular weight), urea, calcium chloride (CaCl2), acetic acid glacial, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, disodium hydrogen phosphate, sodium acetate, orthophosphoric acid, concentrated sulphuric acid, DiAcetylMonoxime (DAM), thiosemicarbazide, sodium chloride and ferric chloride was procured from Hi Media Laboratories Pvt. Ltd. Deionized water was used throughout the experiments.
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