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Mouse il 1β elisa max standard kit

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The Mouse IL-1β ELISA MAX™ Standard Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) for the measurement of mouse interleukin-1 beta (IL-1β) in cell culture supernatants, serum, and plasma. The kit includes the necessary components to perform the assay.

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Lab products found in correlation

Ltb4 eia kit, by Cayman Chemical (3 mentions)

Close spelling variants of this product

Mouse IL-1β ELISA MAX kits IL-1β Mouse ELISA kits ELISAs

3 protocols using mouse il 1β elisa max standard kit

1

Quantification of LTB4 and IL-1β in Tumors

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Subcutaneous tumors were homogenized in 500 ul 1x PBS buffer containing 10uM Indomethacin using Omni GLH general homogenizer. The homogenates were centrifuged at 14000 g for 10 min and LTB4 levels in the supernatants were quantified. The LTB4 levels were normalized to the amount of protein in the supernatants measured by Themo Sceintific Pierce BCA protein assay kit following manufacturer's instructions. LTB4 in the tumor homogenate supernatant was expressed as pg/mg of protein. LTB4 in cell culture supernatants, BALF, air pouch lavage and subcutaneous tumors was measured using LTB4 EIA Kit (Cayman Chemical) according to the manufacturer's instructions. IL-1β in cell culture supernatants was measured using Mouse IL-1β ELISA MAX™ Standard Kit (Biolegend) according to the manufacturer's instructions.
Satpathy S.R., Jala V.R., Bodduluri S.R., Krishnan E., Hegde B., Hoyle G., Fraig M., Luster A.D, & Haribabu B. (2015). Crystalline silica-induced leukotrieneB4-dependent inflammation promotes lung tumor growth. Nature communications, 6, 7064.
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2

Quantification of LTB4 and IL-1β in Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Subcutaneous tumors were homogenized in 500 ul 1x PBS buffer containing 10uM Indomethacin using Omni GLH general homogenizer. The homogenates were centrifuged at 14000 g for 10 min and LTB4 levels in the supernatants were quantified. The LTB4 levels were normalized to the amount of protein in the supernatants measured by Themo Sceintific Pierce BCA protein assay kit following manufacturer's instructions. LTB4 in the tumor homogenate supernatant was expressed as pg/mg of protein. LTB4 in cell culture supernatants, BALF, air pouch lavage and subcutaneous tumors was measured using LTB4 EIA Kit (Cayman Chemical) according to the manufacturer's instructions. IL-1β in cell culture supernatants was measured using Mouse IL-1β ELISA MAX™ Standard Kit (Biolegend) according to the manufacturer's instructions.
Satpathy S.R., Jala V.R., Bodduluri S.R., Krishnan E., Hegde B., Hoyle G., Fraig M., Luster A.D, & Haribabu B. (2015). Crystalline silica-induced leukotrieneB4-dependent inflammation promotes lung tumor growth. Nature communications, 6, 7064.
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3

Quantifying Inflammatory Mediators

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Levels of LTB4 and IL-1β in the supernatants of cell culture and air pouch lavage fluid were measured using LTB4 EIA Kit (Cayman Chemicals) and Mouse IL-1β ELISA MAX Standard Kit (Biolegend) respectively, using manufacturer’s instructions. Absorbance was measured using a BioTek (Winooski, VT) microplate reader at 405nm and 450nm for LTB4 and IL-1β respectively.
Hegde B., Bodduluri S.R., Satpathy S.R., Alghsham R.S., Jala V.R., Uriarte S.M., Chung D.H., Lawrenz M.B, & Haribabu B. (2018). Inflammasome Independent Leukotriene-B4 Production Drives Crystalline Silica Induced Sterile Inflammation. Journal of immunology (Baltimore, Md. : 1950), 200(10), 3556-3567.
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