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Inhibitor 2

Manufactured by Merck Group
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Sourced in United States

Inhibitor II is a laboratory reagent that functions as a protease inhibitor. It is designed to prevent the degradation of proteins during sample preparation and analysis.

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Lab products found in correlation

Ly364947, by Merck Group (1 mentions) Sb431542, by Bio-Techne (1 mentions) Tgf β, by R&D Systems (1 mentions) Pdgf bb, by R&D Systems (1 mentions) Bmp 2, by R&D Systems (1 mentions) Hydrazine, by Merck Group (1 mentions) Lysotracker red, by Thermo Fisher Scientific (1 mentions) Methyl tert butyl ether (mtbe), by Merck Group (1 mentions) Uranyl formate, by Electron Microscopy Sciences (1 mentions) Trifluoroacetic acid tfa, by Merck Group (1 mentions) N n diisopropylethylamine dipea, by Merck Group (1 mentions) Thioanisole, by Merck Group (1 mentions) 4 6 diaminidino 2 pheylindole dapi, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Phosphatase inhibitor cocktails 2 and 3 (210 citations) Phosphatase inhibitors 2 and 3 (19 citations) Phosphatase inhibitors cocktail 2 and 3 (6 citations) Phosphatase inhibitors I and II (6 citations) Phosphatase inhibitors cocktail 2 (4 citations) Phosphatase Inhibitors I&II (3 citations) SIGMAFAST™ Protease Inhibitors and Phosphatase Inhibitor Cocktail 3 and 2 (2 citations) Phosphatase inhibitors (cocktails 2 and 3, (2 citations) Phosphatase inhibitors II & III (2 citations) Phosphatase cocktail inhibitors I and II (2 citations)

2 protocols using inhibitor 2

1

Cytokine Modulation in Cell Culture

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TGF-β, BMP-2, PDGF-BB (R&D Systems, Minneapolis, MN, USA), FGF-2 (Stemgent, Cambridge, MA, USA), and TGF-β receptor inhibitors SB431542 (Tocris Bioscience, Bristol, UK), LY364947 (Sigma-Aldrich, St. Louis, MO, USA), and Inhibitor II (Merck, Darmstadt, Germany) were applied to cell culture media at the concentrations indicated.
Kawahara T., Yamashita M., Ikegami K., Nakamura T., Yanagita M., Yamada S., Kitamura M, & Murakami S. (2015). TGF-Beta Negatively Regulates the BMP2-Dependent Early Commitment of Periodontal Ligament Cells into Hard Tissue Forming Cells. PLoS ONE, 10(5), e0125590.
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2

Peptide Synthesis and Characterization

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All of the protected amino acids, resins, and solvents for peptide synthesis were purchased from Protein Technologies Inc. (Tucson, AZ). Trifluoroacetic acid (TFA), thioanisole, anisole, methyl-tert-butyl ether, N,N-diisopropylethylamine (DIPEA), and hydrazine were obtained from Sigma-Aldrich (St. Louis, MO). N2′-deacetyl-N2′-(3-mercapto-1-oxopropyl)-maytansine (DM1) was supplied by Carbosynth Ltd. (Compton, WB). CathB (purified from bovine spleen) and its inhibitor II were obtained from EMD Millipore (Billerica, MA). Uranyl formate was purchased from Electron Microscopy Sciences (Hatfield, PA). Sulfosuccinimidyl 4-[N-maleimidomethyl]cyclohexane-1-carboxylate (sulfo-SMCC), 4′6-dia-minidino-2-pheylindole (DAPI), and LysoTracker Red were obtained from Life Technologies Inc. (Norwalk, CT).
Bellat V., Lee H.H., Vahdat L, & Law B. (2016). Smart Nanotransformers with Unique Enzyme-Inducible Structural Changes and Drug Release Properties. Biomacromolecules, 17(6), 2040-2049.
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