Developmentally exposed larvae were raised in a dark incubator without a 14:10 light:dark cycle. Following the end of exposure, 5 dpf larvae in the absence of the drug were individually placed in a 96-well plate, pseudo-randomized by treatment, and left in the light for 5 h. The plate was then moved to a DanioVision Observational Chamber (Noldus Information Technology, Wageningen, The Netherlands) where fish movement was recorded over 59 h under different light conditions: 3 h light; 54 h dark; 2 h light. Distance traveled by larvae during the assay was recorded using Ethovision XT software (Noldus Information Technology, Wageningen, The Netherlands) and the data were output in 1-min time bins for analysis. Periodicity was assessed using the R package DiscoRhythm (43 (link)).
Daniovision observational chamber
Manufactured by Noldus
The DanioVision Observational Chamber is a lab equipment product designed for the observation and tracking of small aquatic organisms, such as zebrafish (Danio rerio). The chamber provides a controlled environment for housing and monitoring the behavioral activities of these model organisms.
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Lab products found in correlation
4 protocols using daniovision observational chamber
1
Larval Rhythms: Tracking Behavioral Patterns
2
Larval Zebrafish Locomotion Assay
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Experiments were performed between 1 and 6 p.m. Behavior was assessed at 5 dpf in the presence of the drug, or at 6 dpf, in the absence of the drug, 24 h after the end of the exposure. Larvae were individually placed in 96-well plates, pseudo-randomized by drug treatment, and allowed to acclimate for 30 min. After this period, the plate was placed inside a DanioVision Observational Chamber (Noldus Information Technology, Wageningen, The Netherlands). Larval locomotion was recorded during alternating light/dark periods: 10 min in the dark (infrared conditions), which was used as a baseline; 10 min in the light; 10 min in the dark; 1 min in the light; 10 min in the dark. Distance traveled was recorded using Ethovision XT software (Noldus Information Technology, Wageningen, The Netherlands) and data were output in 10 and 1 s time bins for analysis. Larvae were culled after behavioral experimentation.
3
Acoustic Startle Response in Zebrafish Larvae
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Acoustic startle was performed as described previously (40 (link)) with minor modifications. Experiments were performed between 1 and 6 p.m. Behavior was assessed at 5 dpf in the presence of the drug, or at 6 dpf, in the absence of the drug, 24 h after the end of the exposure. Larvae were individually placed in 96-well plates, pseudo-randomized by drug treatment, and allowed to acclimate for 30 min. After this period, the plate was moved to a DanioVision Observational Chamber (Noldus Information Technology, Wageningen, The Netherlands) where, following a 2-min acclimatization period, larval movement was recorded. Following an initial 1 min baseline period, larvae were subjected to 25 sound/vibration stimuli with 2 s inter-stimulus intervals. The distance traveled was recorded using Ethovision XT software (Noldus Information Technology, Wageningen, The Netherlands), and data were output in 1 s time bins.
4
Circadian Behavior Assay for Larval Zebrafish
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Developmentally exposed larvae were raised in a dark incubator without a 14:10 light:dark cycle. Following the end of exposure, 5 dpf larvae in the absence of the drug were individually placed in a 96-well plate, pseudo-randomized by treatment, and allowed to acclimate in the light for 30 min. The plate was then moved to a DanioVision Observational Chamber (Noldus Information Technology, Wageningen, The Netherlands) where fish movement was recorded over 16.5 h under different light conditions: 3 h light; 46 min gradual dusk; 10 h dark; 46 min gradual dawn; 2 h light. During gradual dusk, light intensity was decreased by 5% every 2 min until 5% intensity was reached and then every 2 min by 1% until the light went off. During gradual dawn, light intensity was increased every 2 min by 1% until 5% intensity was reached and then by 5% until 100% intensity was reached. Distance traveled by larvae during the assay was recorded using Ethovision XT software (Noldus Information Technology, Wageningen, The Netherlands) and the data were output in 1-min time bins for analysis.
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