Haake viscotester iq air

NaHA was dissolved in PBS to prepare 0.5% (w/w) solution with or without 3% (w/v) and 6% (w/v) albumin or HES, and left for 24 h to solidify with gentle stirring at room temperature. Dynamic shear moduli of each NaHA solution were measured by small amplitude oscillatory shear experiments over the frequency range of 0.1–10 Hz with a rotational rheometer (HAAKE Viscotester iQ Air, Thermo Fisher Scientific, Waltham, MA, USA) equipped with a Peltier temperature control module kept at 37 °C. The software HAAKE RheoWin (ver. 4.86, Thermo Fisher Scientific, Waltham, MA, USA) was used to determine storage (G′) and loss (G″) shear moduli of NaHA solution at each frequency. Cone geometry with a diameter of 6 cm and core angle of 2° was used. Prior to frequency sweep experiments, strain amplitude was confirmed by strain sweep experiments to be sufficiently small to provide a linear material response at all investigated frequencies. A solvent trap was used to avoid evaporation of samples during experiments. Experiments were carried out at least six times.

The viscoelasticity and flow behaviour of the monomer itself (CSMA), and CSMA with CaP were measured using a rheometer HAAKE Viscotester iQ air (Thermo Scientific, UK). A 35 mm Titanium parallel plate geometry was used. A rotational ramp with a shear rate between 0.1 s⁻¹ and 100 s⁻¹ and a dynamic oscillatory test with steady deformation ɣ = 1.000 at 1 Hz frequency were undertaken for the samples for a step time of 300 s, all test modes were run at room temperature of 25 °C. Collected data were then analysed on the RheoWin software.

Rheological measurements were performed with the HAAKE Viscotester iQ Air oscillatory rheometer (Thermo Scientific, Bremen, Germany).