Agilent uv visible spectrophotometer

The samples’ total polyphenol (TP) content was determined using the Folin Ciocalteu colorimetric method as described by Blainski et al. [54 (link)] with slight modification. Briefly, 200 µL of the sample aqueous extract was mixed with 300 µL 2% Na₂CO₃ (Fisher Scientific, Waltham, MA, USA), 50 µL Folin-Ciocalten’s reagent (Sigma-Aldrich Co., St. Louis, MO, USA), and 1 mL of distilled water and left for 1 h at room temperature in total darkness. Gallic acid (Sigma-Aldrich, St. Louis, MO, USA) was used as a standard. The absorbance was measured by Agilent UV–visible spectrophotometer (Agilent 8453, Palo Alto, CA, USA) at 765 nm, and the results were expressed as mg Gallic acid equivalents (mg GAE/100 g of the sample).

The required chemicals were purchased from Sigma-Aldrich (Seoul, South Korea) and used as received. The A549 cells for the cytotoxicity study were purchased from the Korea cell line bank, Seoul, South Korea. 3-(4, 5-dimethyl thiazol-2yl)-2, 5-diphenyl tetrazolium bromide (MTB), fetal bovine serum (FBS), trypsin, and dulbecco Modified Eagle Medium (DMEM) were procured from Thermo Fisher Scientific, Waltham, MA, USA. The glass coverslips, cell culture plates, 96 well plates required for cell cytotoxicity assay were obtained from SPL Life Sciences, Seoul, Korea. The FT-IR spectra was recorded in the 4000–400 cm⁻¹ region with a Shimadzu FTIR spectrophotometer (Shimadzu, Kyoto, Japan) using KBr pellet. The ¹H NMR and ¹³C NMR spectra were recorded on a Bruker AVANCE-500 (400 MHz) spectrometer (Bruker, Billerica, UK) using DMSO-d₆ as a solvent. The UV-Visible spectra were recorded on an Agilent UV-Visible spectrophotometer (Agilent Technologies, Santa Clara, CA, USA). Single-crystal X-ray diffraction experiments were performed using an Xcalibur, Sapphire3, Gemini ultra diffractometer equipped with an Oxford Cryosystems 700 Series (Agilent Technologies, Santa Clara, CA, USA) low-temperature apparatus operating at T = 120(2) K. Spectramax Plus 384 (Molecular Devices, San Jose, CA, USA) microplate reader was used for this study.