The luciferase reporter plasmids CRNDE-Wt, CRNDE-Mut, MCM5-Wt and MCM5-Mut were purchased from GeneCreate (Wuhan, China). The sequences were respectively cloned into a pmirGLO Dual-luciferase plasmid to construct the luciferase reporter vectors. Lipofectamine 2000 (Invitrogen, CA, USA) was used to co-transfect the luciferase reporter plasmids and miR-136-5p mimic or mimic negative control into 293T cells. After 48 h culture, the transfected cells were collected and the luciferase activity measured using the Dual-Luciferase Reporter Assay System kit (Promega, WI, USA). The Firefly luciferase activity was standardized by the Renilla luciferase.
Crnde wt
Manufactured by Genecreate
Sourced in China
The CRNDE-Wt is a specialized laboratory equipment designed for genetic research. It is a compact and efficient device that enables researchers to perform essential tasks related to gene analysis and manipulation. The core function of the CRNDE-Wt is to facilitate the investigation of genetic sequences and their properties, providing researchers with a reliable tool to advance their scientific endeavors.
Automatically generated - may contain errors
4 protocols using crnde wt
1
Luciferase Assay for miRNA Targets
2
Luciferase Assay of miR-136 Targets
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The luciferase reporter plasmids CRNDE-Wt, CRNDE-Mut, MCM5-Wt and MCM5-Mut were purchased from GeneCreate (Wuhan, China). The sequences were respectively cloned into a pmirGLO Dual-luciferase plasmid to construct the luciferase reporter vectors. Lipofectamine 2000 (Invitrogen, CA, USA) was used to co-transfect the luciferase reporter plasmids and miR-136-5p mimic or mimic negative control into 293T cells. After 48 h culture, the transfected cells were collected and the luciferase activity measured using the Dual-Luciferase Reporter Assay System kit (Promega, WI, USA). The Fire y luciferase activity was standardized by the Renilla luciferase.
3
Luciferase Assay of miR-136 Targets
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The luciferase reporter plasmids CRNDE-Wt, CRNDE-Mut, MCM5-Wt and MCM5-Mut were purchased from GeneCreate (Wuhan, China). The sequences were respectively cloned into a pmirGLO Dual-luciferase plasmid to construct the luciferase reporter vectors. Lipofectamine 2000 (Invitrogen, CA, USA) was used to co-transfect the luciferase reporter plasmids and miR-136-5p mimic or mimic negative control into 293T cells. After 48 h culture, the transfected cells were collected and the luciferase activity measured using the Dual-Luciferase Reporter Assay System kit (Promega, WI, USA). The Fire y luciferase activity was standardized by the Renilla luciferase.
4
Luciferase Assay for miRNA Targets
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The luciferase reporter plasmids CRNDE-Wt, CRNDE-Mut, MCM5-Wt and MCM5-Mut were purchased from GeneCreate (Wuhan, China). The sequences were respectively cloned into a pmirGLO Dual-luciferase plasmid to construct the luciferase reporter vectors. Lipofectamine 2000 (Invitrogen, CA, USA) was used to co-transfect the luciferase reporter plasmids and miR-136-5p mimic or mimic negative control into 293T cells. After 48 h culture, the transfected cells were collected and the luciferase activity measured using the Dual-Luciferase Reporter Assay System kit (Promega, WI, USA). The Firefly luciferase activity was standardized by the Renilla luciferase.
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