Sa231a2

Manufactured by BioLegend

SA231A2 is a lab equipment product. It functions as a core tool for laboratory research and analysis.

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Lab products found in correlation

Close spelling variants of this product

KLRG1 (SA231A2,

2 protocols using sa231a2

Flow Cytometric Profiling of PBMCs

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Frozen PBMCs were thawed, washed in FACS buffer (2% FBS, 1mM EDTA in PBS), and counted on TC20 (Biorad) before surface staining using two independent flow panels. For the innate panel, the following antibodies were used: CD3 (SP34, BD Pharmingen) and CD20 (2H7, BioLegend) for the exclusion of T & B lymphocytes, respectively. We further stained for CD56 (HCD56, Biolegend), CD57 (HNK-1, BioLegend), KLRG1 (SA231A2, BioLegend) CD16 (3G8, BioLegend), CD14 (M5E2, BioLegend), HLA-DR (L243, BioLegend), CD11c (3.9, ThermoFisher Scientific), CD123 (6H6, BioLegend) and CD86 (IT2.2, BioLegend). For the adaptive panel, the following antibodies were used: CD4 (OKT4, BioLegend), CD8b (2ST8.5H7, Beckman Coulter), CD45RA (HI100, TONBO Biosciences), CCR7 (G043H7, BD Biosciences), CD19 (HIB19, BioLegend), IgD (IA6–2, BioLegend), CD27 (M-T271, BioLegend), KLRG1 (SA231A2, BioLegend) and PD-1 (Eh12.2h7, BioLegend). Cells were stained with Ghost Dye viability dye (TONBO biosciences) for 30 minutes at 4C per manufacturer’s instructions, washed, surface stained with either innate or adaptive panels for 30 minutes at 4C. Samples were then washed and analyzed on Attune NxT Flow Cytometer (ThermoFisher Scientific, Waltham MA).

Sureshchandra S., Zulu M.Z., Doratt B., Jankeel A., Tifrea D., Edwards R., Rincon M., Marshall N.E, & Messaoudi I. (2021). Deep immune profiling of the maternal-fetal interface with mild SARS-CoV-2 infection. bioRxiv.

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SARS-CoV-2 Spike Protein-Specific CD8+ T Cell Analysis

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Cryopreserved PBMCs were thawed and stained with APC-labeled HLA-A*0201 pentamers (YLQPRTFLL: SARS-CoV-2 S₂₆₉) and antibodies. For pentamer staining, the cells were incubated for 15 min at room temperature, washed, and then stained with fluorochrome-conjugated antibodies for specific surface markers for 20 min at 4°C. The antibody mixture consisted of anti-human CD3 (Biolegend, clone HIT3a), CD8α (Biolegend, clone HIT8a), CCR7 (Biolegend, clone G043H7), CD45RA (Biolegend, clone HI100), KLRG1 (Biolegend, clone SA231A2), CD127 (Biolegend, clone A019D5), CD28 (Biolegend, clone CD28.2), PD-1 (Biolegend, clone EH12.2H7), CD38 (Biolegend, clone HIT2), HLA-DR (Biolegend, clone L234), and CD107a (Biolegend, clone H4A3). After three additional washes with FACS buffer, the cells were analyzed on a FACSCanto II (BD Biosciences).

Kondo H., Kageyama T., Tanaka S., Otsuka K., Tsukumo S.I., Mashimo Y., Onouchi Y., Nakajima H, & Yasutomo K. (2022). Markers of Memory CD8 T Cells Depicting the Effect of the BNT162b2 mRNA COVID-19 Vaccine in Japan. Frontiers in Immunology, 13, 836923.

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