Poly mount xylene

Manufactured by Polysciences

Sourced in United States

Poly-mount xylene is a solvent-based mounting medium designed for use in microscopy. It is formulated to provide a clear, durable mounting solution for microscope slides. Poly-mount xylene is a versatile product that can be used with a variety of specimen types.

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Lab products found in correlation

Cryostat, by Leica (1 mentions) Paraformaldehyde (pfa), by Thermo Fisher Scientific (1 mentions) Mayer s hematoxylin solution, by Merck Group (1 mentions) Eosin y solution, by Merck Group (1 mentions) Dfc300fx camera, by Leica (1 mentions) Mz16f stereoscope, by Leica (1 mentions)

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Poly-Mount (28 citations)

6 protocols using poly mount xylene

Histological Staining of Spinal Cord

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Spinal cord tissue section slides were dried at room temperature for 2 hr. Following drying, slides were rehydrated in 3-min baths of xylene, 100% ethanol, 95% ethanol, 70% ethanol and dH₂O. To stain the tissue, slides were placed in an Eriochrome solution (0.16% Eriochrome Cyanine, 0.4% Sulfuric Acid, 0.4% Ferric Chloride in dH₂O) for 14 min, washed with tap water, placed in a developing solution (0.3% ammonium hydroxide in dH₂O) for 5 min, washed with dH₂O, and then placed into a cresyl violet solution (0.4% cresyl violet, 6% 1 M sodium acetate, 34% 1 M acetic acid) for 18 min. After staining, slides were dehydrated by being placed in baths of dH₂O, 70% ethanol, 95% ethanol, 100% ethanol and xylene. Slides were mounted with poly-mount xylene (Polysciences, Warrington, Pennsylvania), and cover slips were added. Slides were then kept at room temperature for storage and analysis.

Urban M.W., Charsar B.A., Heinsinger N.M., Markandaiah S.S., Sprimont L., Zhou W., Brown E.V., Henderson N.T., Thomas S.J., Ghosh B., Cain R.E., Trotti D., Pasinelli P., Wright M.C., Dalva M.B, & Lepore A.C. (2024). EphrinB2 knockdown in cervical spinal cord preserves diaphragm innervation in a mutant SOD1 mouse model of ALS. eLife, 12, RP89298.

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Tissue Staining with Eriochrome Cyanine

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Tissue sections were retrieved from the −20°C freezer and dried at room temperature for 2 hours. Slides were then placed in 3-minute baths of xylene, 100% ethanol, 95% ethanol, 70% ethanol and dH₂O. Slides were next placed in an ErioChrome solution (0.16% Eriochrome Cyanine, 0.4% Sulfuric Acid, 0.4% Ferric Chloride in dH₂O) for 14 minutes, washed with tap water, placed in a developing solution (0.3% ammonium hydroxide in dH₂O) for 5 minutes, washed with dH₂O, and then placed into a cresyl violet solution (0.4% cresyl violet, 6% 1M sodium acetate, 34% 1M acetic acid) for 18 minutes. Finally, slides were placed in baths of dH₂O, 70% ethanol, 95% ethanol, 100% ethanol and xylene. Slides were then mounted with poly-mount xylene (Polysciences, Warrington, Pennsylvania) and cover slips were added. Slides were then kept at room temperature for analysis.

Urban M.W., Ghosh B., Strojny L.R., Block C.G., Blazejewski S.M., Wright M.C., Smith G.M, & Lepore A.C. (2018). Cell-type specific expression of constitutively-active Rheb promotes regeneration of bulbospinal respiratory axons following cervical SCI. Experimental neurology, 303, 108-119.

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Muscle Tissue Cryosectioning and H&E Staining

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Collected muscle samples were frozen in liquid nitrogen-chilled isopentane for 30 seconds. Cross sections were then obtained on a Leica cryostat at a thickness of 10 μm. Sections were air-dried for 30 minutes at room temperature and then fixed in 4% (w/v) of paraformaldehyde (Thermo Scientific) for 10 minutes followed by washing in 1× PBS for 5 minutes. Hematoxylin and Eosin (H&E) staining was then performed as following: 1) stained in Mayer’s Hematoxylin solution (Millipore Sigma, Burlington, MA, USA.) for 10 minutes, 2) rinsed in warm running tap water for 15 minutes, 3) washed in distilled water for 30 seconds, 4) placed in 95% (v/v) reagent alcohol for 30 seconds, 5) stained in Eosin Y solution (Alcoholic, Millipore Sigma) for 1 minute, 6) dehydrated and cleared through 2 changes each of 95% reagent alcohol, absolute reagent alcohol, and xylene for 2 minutes each, 7) mounted with Poly Mount Xylene (Polysciences, Warrington, PA, USA). Stained sections were viewed and photographed under a bright-field microscope (Nikon, Y-THR-L).

Wang X., Chen J., Homma S.T., Wang Y., Smith G.R., Ruf-Zamojski F., Sealfon S.C, & Zhou L. (2022). Diverse effector and regulatory functions of fibro/adipogenic progenitors during skeletal muscle fibrosis in muscular dystrophy. iScience, 26(1), 105775.

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Tissue Staining and Mounting Protocol

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Tissue sections were dried at room temperature for 2 h. Slides were then placed in 3-min baths of xylene, 100% ethanol, 95% ethanol, 70% ethanol, and dH₂O. Slides were next placed in an eriochrome solution (0.16% eriochrome cyanine, 0.4% sulfuric acid, 0.4% ferric chloride in dH₂O) for 14 min, washed with tap water, placed in a developing solution (0.3% ammonium hydroxide in dH₂O) for 5 min, washed with dH₂O, and then placed into a cresyl violet solution (0.4% cresyl violet, 6% 1 M sodium acetate, and 34% 1 M acetic acid) for 18 min. Finally, slides were placed in baths of dH₂O, 70% ethanol, 95% ethanol, 100% ethanol, and xylene. Slides were then mounted with poly-mount xylene (Polysciences), and cover slips were added. Slides were then kept at room temperature for analysis.

Urban M.W., Ghosh B., Block C.G., Strojny L.R., Charsar B.A., Goulão M., Komaravolu S.S., Smith G.M., Wright M.C., Li S, & Lepore A.C. (2019). Long-Distance Axon Regeneration Promotes Recovery of Diaphragmatic Respiratory Function after Spinal Cord Injury. eNeuro, 6(5), ENEURO.0096-19.2019.

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Histological Analysis of Carnoy's Fixed Glands

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Tissue sections from Carnoy’s fixed glands were deparaffinized and rehydrated through a series of ethanols, and then stained with H&E or Masson’s trichrome as described previously (https://www.bcm.edu/research/labs/jeffrey-rosen/protocols). The stained sections were mounted in Poly-Mount Xylene (Polysciences, Inc) and imaged using an Olympus light microscope (BX40) with an Olympus HIH-035382 camera.

Shore A.N., Chang C.H., Kwon O.J., Weston M.C., Zhang M., Xin L, & Rosen J.M. (2015). PTEN is required to maintain luminal epithelial homeostasis and integrity in the adult mammary gland. Developmental biology, 409(1), 202-217.

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Visualization of Murine Mammary Glands

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The fourth pair of mammary glands were harvested from mice and spread onto glass slides. Next, they were fixed in Carnoy’s Fix (6:3:1 mixture of 100% ethanol, chloroform, and glacial acetic acid) for 1–4 hr, and stained in carmine-alum stain overnight. The stained glands were washed and dehydrated in 70%, 95%, and 100% ethanol for 1 hr each, and then transferred to xylene overnight for clearing. They were mounted in Poly-Mount Xylene (Polysciences, Inc) and imaged using a Leica MZ16F Stereoscope with a Leica DFC300FX camera.

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