Tetramer staining was adapted from Tungatt et al. (Tungatt et al., 2015 (link)). Cells were treated with dasatinib (AdooQ Bioscience) at a final concentration of 50 nM for 30 minutes at 37°C. PE- and APC-labeled tetramers were added directly to dasatinib-treated cells (without washing) at a final concentration of 12.5–100 nM for 1 hour at room temperature. Cells were washed and incubated with unconjugated mouse anti-PE antibody (clone PE001, Biolegend) and mouse anti-APC antibody (clone APC003, Biolegend) at a concentration of 10 µg/mL for 20 minutes on ice. Cells were washed and stained for flow cytometric analysis as described below. Staining of cells from Aire+/+ and Aire−/− prostates was performed with 12.5 nM of each tetramer. Staining of cells from TRAMP prostate tumors was performed with 20 nM of each tetramer.
Dasatinib
Manufactured by Adooq
Sourced in China
Dasatinib is a tyrosine kinase inhibitor used in research applications. It is a selective inhibitor of the Bcr-Abl, Src, c-Kit, PDGFR, and Ephrin receptor tyrosine kinases.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti apc antibody clone apc003, by BioLegend (1 mentions)
Trametinib, by Adooq (1 mentions)
Ruxolitinib, by Adooq (1 mentions)
Afatinib, by Adooq (1 mentions)
Lenvatinib, by Adooq (1 mentions)
Regorafenib, by LGC (1 mentions)
Gefitinib, by Cayman Chemical (1 mentions)
Elacridar, by LGC (1 mentions)
Ponatinib, by Adooq (1 mentions)
Dabrafenib, by Adooq (1 mentions)
Carfilzomib, by Adooq (1 mentions)
Vemurafenib, by Adooq (1 mentions)
Ko143, by Merck Group (1 mentions)
Vandetanib, by Adooq (1 mentions)
Dacomitinib, by Adooq (1 mentions)
Lapatinib, by Adooq (1 mentions)
Bortezomib, by Adooq (1 mentions)
Pazopanib, by Adooq (1 mentions)
Erlotinib, by Cayman Chemical (1 mentions)
Sorafenib, by LKT Laboratories (1 mentions)
3 protocols using dasatinib
1
Tetramer Staining Protocol for T Cell Analysis
2
Dasatinib Inhibits Abelson Tyrosine Kinase in Silkworms
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Abelson tyrosine kinase was inhibited by Dasatinib (Adooq Bioscience, Nanjing, China). An amount of 1 mg of Dasatinib was dissolved in 205 mL H2O to prepare the stock solution. The stock solution was diluted to 0.6 nm and smeared on the fresh mulberry leaves. First-day third-instar larvae of B. mori were reared with inhibitor-treated leaves until the wandering stage. The Control group larvae were reared with H2O-treated leaves. Each group had three biological replicates. Each replicate contained thirty larvae. All larvae were taken for the same treatment.
3
Cell-based Assay of Multi-Targeted Kinase Inhibitors
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Regorafenib, M-2, M-5, and elacridar were purchased from Toronto Research Chemicals (North York, ON). Ko143 was from Sigma-Aldrich (St. Louis, MO). Imatinib mesylate was from Focus Biomolecules (Plymouth Meeting, PA). Afatinib, bortezomib, cabozantinib, carfilzomib, dabrafenib, dacomitinib, dasatinib, lapatinib, lenvatinib, pazopanib, ponatinib, ruxolitinib, tofacitinib, trametinib, vandetanib, and vemurafenib were from AdooQ Bioscience (Irvine, CA). Sorafenib was from LKT Laboratories Inc. (St. Paul, MN). Sunitinib was from Synkinase Pty Ltd. (San Diego, CA). Crizotinib was from LC Laboratories Inc. (Woburn, MA). Gefitinib and erlotinib were from Cayman Chemical Company (Ann Arbor, MI). Nilotinib was from ChemScene, LLC (Monmouth Junction, NJ). All other chemicals and reagents were of analytical grade and were obtained from commercial sources.
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