Recombinant human ifn β beta 300 02bc

Recombinant human IFN-β (beta) (300-02BC) and human IFN-λ (lambda) (300-02 L) were obtained from PeproTech and were used to test IFN sensitivity of full-length, authentic SARS-CoV-2 variants (IFN was added 18 h prior to inoculation and removed prior to virus addition). Recombinant human IFN-β (beta) 1a (11415-1) and human IFN-λ (lambda) (11725-1) were obtained from PBL Assay Science and were used to test IFN sensitivity of VSV-based pseudoviruses (IFN was added 18 h prior to inoculation and removed prior to virus addition). The following inhibitors were reconstituted in DMSO: E64d (Sigma; E8640), camostat mesylate (Sigma; SML0057), incyclinide (MedChemExpress; HY-13648), apratastat (MedChemExpress; HY-119307), batimastat (SelleckChem: S7155), EG00229 (SelleckChem; E1119). Bafilomycin A1 (Sigma; SML1661) was received as a ready-made solution in DMSO, and amphotericin B (Sigma; A2942) was received as a ready-made solution in deionized water.

Recombinant human IFN-β (beta) (300–02BC) and human IFN-λ (lambda) (300–02L) were obtained from PeproTech and were used to test IFN sensitivity of full-length, authentic SARS-CoV-2 variants (IFN was added 24 hours prior to inoculation and removed prior to virus addition). Recombinant human IFN-β (beta) 1a (11415–1) and human IFN-λ (lambda) (11725–1) were obtained from PBL Assay Science and were used to test IFN sensitivity of VSV-based pseudoviruses (IFN was added 24 hours prior to inoculation and removed prior to virus addition). The protease inhibitor E64d (E8640) was obtained from Sigma and reconstituted in DMSO. The serine transmembrane protease inhibitor camostat mesylate (SML0057) was obtained from Sigma and reconstituted in DMSO. The MMP inhibitor incyclinide (HY-13648) was obtained from MedChemExpress. The endolysosomal acidification inhibitor bafilomycin A1 (SML1661) was obtained from Sigma as a ready-made solution in DMSO.

Recombinant human IFN-β (beta) (300-02BC) and human IFN-λ (lambda) (300-02L) were obtained from PeproTech and were used to test IFN sensitivity of full-length, authentic SARS-CoV-2 variants (IFN was added 18 hours prior to inoculation and removed prior to virus addition). Recombinant human IFN-β (beta) 1a (11415-1) and human IFN-λ (lambda) (11725-1) were obtained from PBL Assay Science and were used to test IFN sensitivity of VSV-based pseudoviruses (IFN was added 18 hours prior to inoculation and removed prior to virus addition). The following inhibitors were reconstituted in DMSO: E64d (Sigma; E8640), camostat mesylate (Sigma; SML0057), incyclinide (MedChemExpress; HY-13648), apratastat (MedChemExpress; HY-119307), batimastat (SelleckChem: S7155), EG00229 (SelleckChem; E1119). Bafilomycin A1 (Sigma; SML1661) was received as a ready-made solution in DMSO and amphotericin B (Sigma; A2942) was received as a ready-made solution in deionized water.