Total activity measurements of freely moving mice were made every 30 days after PLX dosing in Promethion cages (Sable Systems International). At each time point, mice were first randomized and placed individually in Promethion cages for 4 to 6 days. Real-time cage activity recording was continuous during the entire session using a combination of a running wheel with sensors to measure speed and distance traveled, three balances to measure body weight, food and water consumption, and a matrix of infrared light beams to measure XYZ movements with 0.25 cm resolution. Analysis of these metrics was used to detect behaviors such as sleep, rearing and general locomotion. For each mouse, data used for analyses were average readings per light or dark cycle. Data from the first circadian cycle were excluded due to variable behavior during habituation. To calculate the activity scores, wheel use, locomotion and rearing were first normalized to a 0–1 scale by the maximum value in the whole dataset, and then the geometric mean of the normalized values for each session was calculated.
Promethion cages
Manufactured by Sable Systems
Sourced in United States
Promethion cages are a series of laboratory animal enclosures designed for housing small rodents, such as mice and rats, in a controlled environment. The cages provide a secure and regulated space for housing and monitoring these animals during scientific research and experiments.
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Lab products found in correlation
5 protocols using promethion cages
1
Comprehensive Locomotion Monitoring of Mice
2
Metabolic Effects of NanoSOD in Mice
3
Urinary Albumin-Creatinine Ratio in Rats
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The rats in each group were fed in the promethion cages (Sable Systems International, USA), and 24 h urine was collected 4 weeks later. After the total amount of urine was determined, 3 mL of urine was centrifuged at 3,000 rpm for 10 min at 4°C, and the supernatant was taken. The 24 h microalbumin (mALB) in urine was detected using an automatic biochemical analyzer (Olympus, Japan) to calculate the urinary albumin/creatinine ratio (ACR).
4
Body Composition Analysis of Transgenic Mice
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Posterior subcutaneous fat pads and epididymal fat pads were excised, weighed and expressed as % body weight. Body composition analyses and indirect calorimetry were performed using Promethion cages (Sable Systems International) at the Vanderbilt Mouse Metabolic Phenotyping Center by monitoring 30-week-old K14-VEGF-C and WT controls for 2 weeks in single housed cages.
5
Automated Monitoring of Mouse Behaviors
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Total activity measurements of freely moving mice were made every 30 days after PLX dosing in Promethion cages (Sable Systems International). At each time point, mice were first randomized and placed individually in Promethion cages for 4 to 6 days. Real-time cage activity recording was continuous during the entire session using a combination of a running wheel with sensors to measure speed and distance traveled, three balances to measure body weight, food and water consumption, and a matrix of infrared light beams to measure XYZ movements with 0.25 cm resolution. Analysis of these metrics was used to detect behaviors such as sleep, rearing and general locomotion. For each mouse, data used for analyses were average readings per light or dark cycle. Data from the first circadian cycle were excluded due to variable behavior during habituation. To calculate the activity scores, wheel use, locomotion and rearing were first normalized to a 0-1 scale by the maximum value in the whole dataset, and then the geometric mean of the normalized values for each session was calculated.
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