L phenylephrine

Ang-[1–10], Ang-[1–8], Ang-[1–7], captopril (Cap), losartan, inactin, L-phenylephrine and Evans blue dye were purchased from Sigma (St. Louis, MO). Isoflurane was purchased from Cristália Ltda (Itapira, SP, Brazil). Ketoprofen (Ketoflex) was purchased from Mundo Animal (Pindamonhangaba, SP, Brazil). Penicillin, streptomycin, and dihydrostreptomycin were purchased from Fort Dodge Animal Health (Hialeah, FL, USA). Abz-Phe-Arg-Lys(Dnp)-Pro-OH was purchased from GenScript (Piscataway, NJ, USA). Mca-Ala-Pro-Lys(Dnp)-OH was purchased from Enzo Life Science (Farmingdale, NY, USA). MLN-4760 was purchased from EMD Milllipore (Billerica, MA, USA). Ceramic microbeads were purchased from MP Biomedicals (Solon, OH, USA). RNeasy was purchased from Qiagen (Germantown, MD, USA). RNase-free water, SYBR green supermix, and high-capacity cDNA reverse transcription kits (#1708890) were purchased from Bio-Rad (Hercules, CA, USA). Renin activity 520 Rat Renin Fluorimetric assay kit was purchased from SensoLyte (AnaSpec, San Jose-CA). Angiotensinogen Elisa kit was purchased from IBL (Hamburg, Germany). Aldosterone Elisa kit was purchased from Enzo Life Science (Farmingdale, NY, USA).

The baroreflex is induced by successive administration of diltiazem (Sigma) at 1 mg/kg into the penis vein, followed by infusion of L-phenylephrine (Sigma) at 20 µg/kg/min into the jugular vein. The baroreflex curve is constructed by plotting the RNA/pressure from the lowest pressure after diltiazem injection to the highest pressure after phenylephrine administration. For each rat, the RNA values are averaged by steps of 5 mmHg; therefore, the values can be averaged in each group of rats (n = 9 WKY, n = 9 SHR, and n = 10 SHRLN).
The Bezold–Jarisch reflex is elicited by a rapid injection of m-chlorophenylbiguanide hydrochloride (1-3-20 µg/kg) (CPBG, RBI Biochemicals) via the right jugular vein, close to the heart, in an additional group of rats (n = 7 WKY, n = 5 SHR, and n = 7 SHRLN).

Sodium citrate, citric acid, L-phenylephrine, formalin, N-benzoyl-arginine-p-nitroanilide, trypsin from porcine pancreases, hyaluronic acid sodium salt from Streptococcus equi, hexadecyltrimethylammonium, hyaluronidase from bovine testes type IV-S, and sinapinic acid (>99.0% purity) were obtained from Sigma (USA). Refined chemicals used for buffer preparations, deionized water, and HPLC grade water were purchased from J.T. Baker (USA). All reagents used in the determination of protein concentration and in electrophoretic analysis were obtained from Bio-Rad (USA).