All experiments were conducted adhering to the Ethical Guideline Regulation of the International Association for the Study of Pain (IASP) and approved by the local ethical and scientific institutional review board at the Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz in Mexico City; (Project: INPRFM-NC-3230 for FP). Vital signs were constantly monitored: body temperature was measured by a rectal probe and maintained at 37 ± 0.5 °C during all measurements by means of a thermal isolation device, respiration was monitored by a balloon secured on the rat’s chest and a pressure transducer as well as an electrocardiogram were used to monitor (model 1025, SA Instruments, Inc., Stony Brook, NY., USA). The animals were continuously anesthetized with isoflurane 2.5% in a mixture of O2 via a nosecone for the entirety of the experiment. Once anesthetized, they were fixed in a prone position in a rat cradle equipped with a stereotactic-like head holder. Before positioning the animals in the scanner, a nylon filament (3–0) was fixed around the left sciatic nerve to perform the denervation. Also, in the same ankle, an input/output cannula circuit to a latex sleeve enveloping the left rear ankle was placed.
Model 1025
Manufactured by SA Instruments
Sourced in United States
The Model 1025 is a precision laboratory instrument designed for measuring and analyzing various physical and electrical properties. It features a high-performance data acquisition system and advanced signal processing capabilities. The core function of the Model 1025 is to provide accurate and reliable measurements for research and testing applications.
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Lab products found in correlation
Magnetom lumina, by Siemens (3 mentions)
Waterbed, by SA Instruments (2 mentions)
Mr compatible small animal heating system, by SA Instruments (2 mentions)
Mr compatible small animal monitoring and gating system, by SA Instruments (2 mentions)
Mouse scapula array p h04le 030 version1, by Rapid Biomedical (2 mentions)
7t mri scanner, by Bruker (1 mentions)
Paravision 5, by Bruker (1 mentions)
T9562, by Bruker (1 mentions)
Paravision 6, by Bruker (1 mentions)
Quadrature volume resonator, by Bruker (1 mentions)
Gradient insert coil, by Rapid Biomedical (1 mentions)
Osirix, by Pixmeo (1 mentions)
Somatom definition flash, by Siemens (1 mentions)
7.0t clinscan, by Bruker (1 mentions)
Isoflurane, by CP-Pharma (1 mentions)
Paravision 3.0.2 acquisition platform, by Bruker (1 mentions)
Biograph mmr, by Siemens (1 mentions)
Mimics software, by Materialise (1 mentions)
Isoflurane, by Abbott (1 mentions)
39 protocols using model 1025
1
Sciatic Nerve Denervation Protocol
2
In Vivo Murine MRI at 7T
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In vivo magnetic resonance imaging was performed using a 7.0‐Tesla small animal magnetic resonance scanner (Bruker PharmaScan, Ettlingen, Germany). Mice were anesthetized by a continuous supplying of 1% isoflurane. Respiratory rate and body temperature were monitored by a physiology monitor (Model 1025; SA Instruments Inc, Stony Brook, NY). T2‐weighted images were acquired using a turbo‐rapid acquisition with refocused echo/T2 sequence, and the parameters were as follows: repetition time, 2500 ms; echo time, 50 ms; field of view, 2.0×2.0 cm; flip angle, 180 degrees; matrix size, 256×256; and slice thickness, 1 mm.
3
In Vitro and In Vivo MRI Analysis of IONC
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MRI studies were performed using a Bruker 7.0 T ClinScan high-field small animal MRI system with a commercial rat coil (Bruker Biospin). In vitro R2 star was performed after coincubation of IONC with/without AA at room temperature for 4 h (The molar ratio of Fe to AA approximately is 1:2). During in vivo test, body temperature was monitored continuously and controlled with a waterbed (SA Instruments, Stony Brook, NY). T2-weighted images were collected pre- and post-arterial infusion of the HCSVs (50 μL, 10 mg/mL). MR scans were performed using a gradient-echo sequence with following parameters: TR/TE = 1,300/7.2 ms, 1 mm slice thickness, FOV 71 × 85 mm, 216 × 256 matrix, respiratory triggering with MRI-compatible small animal gating system (Model 1025, SA Instruments, Stony Brook, NY).
4
Small Animal MRI Imaging Protocol
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Imaging was performed in supine position with a clinical 3T Siemens MRI scanner (Biograph mMR, Siemens-Healthcare-Solutions, Germany) using a 4 channel receive coil array for small animal imaging (Mouse Heart Array, Rapid Biomedical GmbH, Germany). An overview of the MR scan parameters can be obtained from Table 1 . To avoid cooling and reduced body temperature during MRI, the body temperature (37°C) of the mice was monitored using a MR-compatible heating system (Model 1025, SA Instruments Inc, NY). For venous access, a small diameter tube with a 30G cannula attached to it was used, which allowed the injection of contrast medium into the tail vein.
5
Rat Brain Infarct Quantification via MRI
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Animal MRI scans were performed using a 7.0 T MRI scanner (BioSpec 70/20 USR, Bruker Biospin Gmbls, Germany). After rats were anesthetized with isoflurane (2.5%), their body temperature was controlled with a water circulating heating bed (SA Instruments, Stony Brook, NY) and respiratory rate was monitored during scanning with MRI-compatible small animal gating system (Model 1025, SA Instruments, Stony Brook, NY). T2-weighted images (T2WI) were collected at Days 3 and 7 after EA. T2WI scans were performed using a gradient-echo sequence with following parameters: TR/TE = 4200/35 ms, FOV = 32 × 32 mm, Averages = 2, Matrix = 256 × 256, Slices = 21, Slice Thickness = 0.8 mm. The infarct volume was calculated over all slices (volume of lesion/total brain volume) using Image J and expressed as a percentage of total brain volume.
6
In Vivo Xenograft Tumor Imaging on 7T MRI
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We imaged 22 xenograft mice (7 MCF-7 and 15 MDA-MB-231) on a 7 T MRI scanner (Bruker Biospin, Ettlingen, Germany) using a 1H quadrature transmit/receive volume coil. The mice were anesthetized with 1–3% isoflurane in air and were kept still inside the magnet using ear bars and a bite bar connected to a nose cone. Respiration and rectal temperature were continuously monitored using an MR-compatible monitoring system (Model 1025, SA Instruments, Inc., Stony Brook, NY, USA). The rectal temperature was maintained at 34–37 °C.
7
Longitudinal MRI of Experimental Autoimmune Encephalomyelitis
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Animals were anesthetized with a mixture of 1%–1.5% isofluorane (Abbott GmbH & Co.), air, and oxygen, and they were placed on an animal holder, kept warm with circulating water. Body temperature and respiration rate were monitored continuously with a remote monitoring system (Model 1025, SA Instruments Inc.). MRI was performed (Supplemental Methods ), keeping slice positioning constant through the experiment, positioned parallel to the base of the brain. Mice were imaged at baseline, 2 days before immunization, and every 2–3 days after immunization, until day 64 after EAE induction. A total of n = 9 mice underwent the full longitudinal examination. We confirmed that the signal-to-noise and contrast-to-noise ratio (between cortex and ventricles) were consistent throughout the entire study period.
8
High-field MRI of Embryonic and Fetal Hearts
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A 7T preclinical MR system (BioSpec 70/20 USR, Bruker BioSpin MRI GmbH) installed with ParaVision 5.1 software (Bruker BioSpin) was used for MRI.26 According to the size of the specimens, 2 different conditions were selected for data acquisition (Table S1 ). A transmit‐receive solenoid coil (inner diameter, 19 mm; Takashima Seisakusho Co., Ltd.) was used for the dissected hearts an embryonic and small fetal samples (CRL, 18–32 mm; 8–12 WGA), and a circular polarized transmit‐receive volume coil (inner diameter, 72 mm, T9562; Bruker BioSpin) was used for large fetal samples (CRL, 43–160 mm; 11–24 WGA). During MR measurements, sample temperature was regulated at 21°C by controlling the temperature of the air supplied in the magnet bore via a heater system (MR‐compatible small animal heating system; SA Instruments Inc.). The temperature of samples was monitored using a thermistor temperature probe and monitoring system (Model 1025, MR‐compatible small animal monitoring and gating system; SA Instruments Inc.).
9
Multimodal Cardiac MRI Phenotyping in Murine Ischemia-Reperfusion
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ECG electrodes were placed at the front paws to monitor the heart rate, which was maintained at 400–600 bpm by adjusting the isoflurane level. A respiratory sensor balloon was placed on the abdomen and the respiratory rate was kept at 70 ± 10 bpm (SA Instruments Model 1025, Stony Brook, NY, USA). The mouse was placed in the magnet so that the heart was positioned at the isocenter of the magnet in the middle of the phased array coil. Retrospective triggering was used to distinguish cardiac and respiratory phases from the images after acquisition. For healthy baseline and mice at days 3 and 21 after I/R, one imaging session accounted for DCE, T1-mapping, tagging and volumetric Cardiac MRI (5–7 days prior to I/R surgery). For day 1 after I/R injury, the imaging session was minimized to only tagging and LGE MRI, to diminish the time under anesthesia and to consequently optimize survival. At day 3 and 22 after I/R, DCE, T1-mapping, tagging and volumetric MRI were performed again (Fig. 1e ).
10
In vivo MRI Imaging of Mice
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In vivo MR imaging was performed using a 3.0 Tesla MR scanner (MAGNETOM Lumina, Siemens, Erlangen, Germany) and a 4-channel receive coil array for mouse body applications (Mouse scapula Array, P-H04LE-030, version1, Rapid Biomedical GmbH, Germany). After induction of i.p. anesthesia, the mice were placed in a prone position on the MRI patient table. A venous port was placed via the tail vein for the administration of the contrast agent during MR imaging. The temperature of the body (37 °C) was monitored with an MR-compatible heating system (model 1025, SA Instruments Inc, Stony Brook, NY, USA) to prevent rapid cooling.
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