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Skanit for multiscan fc software

Manufactured by Thermo Fisher Scientific
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Sourced in Germany, Japan

SkanIt for Multiscan FC software is a data analysis software designed to work with Thermo Scientific Multiscan FC microplate reader. The software is used to control the instrument and analyze data generated from microplate-based experiments.

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Lab products found in correlation

Multiscan fc, by Thermo Fisher Scientific (2 mentions) Cell counting kit 8 cck 8 reagent, by Dojindo Laboratories (1 mentions) Multiscan fc plate reader, by Thermo Fisher Scientific (1 mentions)

3 protocols using skanit for multiscan fc software

1

Cytokine and Angiogenic Factor Quantification

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Tumor necrosis factor-alpha (TNF-a), interleukin (IL)-1β, IL-6, PRDX2 and VEGF secretion in cell culture supernatants or serum were measured by ELISA, according to the manufacturer’s standard protocols (eBioscience, San Diego, CA). Absorbance was read on a Multiscan FC plate reader and analyzed with Skan It for Multi scan FC software (Thermo Scientific, Schwerte, Germany).
Jin X., Chen C., Li D., Su Q., Hang Y., Zhang P, & Hu W. (2017). PRDX2 in Myocyte Hypertrophy and Survival is Mediated by TLR4 in Acute Infarcted Myocardium. Scientific Reports, 7, 6970.
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2

Cell Proliferation Assay in U2OS and MG63 Cells

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U2OS and MG63 cells were first seeded into 96‐well tissue culture plates (3 × 103 cells per well). The detection was performed at 0, 24, 48 and 72 hours. After adding 10 µL of Cell Counting Kit‐8 (CCK8) reagent (Dojindo Molecular Technologies, Inc, Kumamoto, Japan) per well and incubating for 2 hours, the absorbance at 450 nm was measured using a Multiscan FC plate reader and analysed with SkanIt for Multiscan FC software (Thermo Scientific).
Xie W., Xiao J., Wang T., Zhang D, & Li Z. (2019). MicroRNA‐876‐5p inhibits cell proliferation, migration and invasion by targeting c‐Met in osteosarcoma. Journal of Cellular and Molecular Medicine, 23(5), 3293-3301.
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3

Quantification of Cytokines, AGEs, and HMGB1

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Cytokines (TNF-α, IL-6, and IL-10) in cell culture supernatants or serum were measured by ELISA (eBiosciences, San Diego, CA). AGEs and HMGB1 were quantitated using ELISA kits from MyBioSource Inc. (San Diego, CA), and IBL International Corp. (Toronto, ON, CA), respectively, according to manufacturers’ protocols. Data were acquired with a Multiscan FC plate reader and analyzed with SkanIt for Multiscan FC software (Thermo Scientific).
Yue S., Zhou H., Zhu J., Rao J., Busuttil R.W., Kupiec-Weglinski J.W., Lu L, & Zhai Y. (2015). Hyperglycemia and Liver Ischemia Reperfusion Injury: A Role of the Advanced Glycation Endproduct and Its Receptor Pathway. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 15(11), 2877-2887.
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