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Cd4 pe cy7 sk3 cd8 percp cy5.5 rpa t8 ccr7 fitc g043h7 cd45ra bv421 hi100 hla dr pe l243 cd38 bv605 hit2

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CD4 PE-Cy7 (SK3), CD8 PerCP-Cy5.5 (RPA-T8), CCR7 FITC (G043H7), CD45RA BV421 (HI100), HLA-DR PE (L243), CD38 BV605 (HIT2) are flow cytometry reagents used to identify and characterize immune cell populations. Each reagent consists of a fluorescently-conjugated monoclonal antibody that binds to a specific cell surface marker.

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Lab products found in correlation

Human trustain fcx blocking buffer, by BioLegend (2 mentions) Horizon brilliant stain buffer, by BD (2 mentions) Facsverse, by BD (2 mentions)

Close spelling variants of this product

CD45RA PerCP-Cy5.5 (HI100), HLA-DR BV605 (L243; CD8 BV605 (RPA-T8; PerCp/Cy5.5-HLA DR CCR7-PE-Cy7 (G043H7, CD8(RPA-T8)-FITC HLA-DR PerCP/Cy5.5 CD4 PE-Cy7 (SK3, HLA-DR-PE-Cy5 HLA-DR PE-Cy7

2 protocols using cd4 pe cy7 sk3 cd8 percp cy5.5 rpa t8 ccr7 fitc g043h7 cd45ra bv421 hi100 hla dr pe l243 cd38 bv605 hit2

1

Flow Cytometric Analysis of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For flow cytometric analyses, Fc-receptors were blocked with Human Trustain FcX blocking buffer (Biolegend, USA) for 5 minutes at room temperature, and cells were then stained with CD4 PE-Cy7 (SK3); CD8 PerCP-Cy5.5 (RPA-T8); CCR7 FITC (G043H7); CD45RA BV421 (HI100); HLA-DR PE (L243); CD38 BV605 (HIT2) (all from Biolegend, USA). Antibodies were mixed in BD HorizonTM Brilliant Stain Buffer (BD Biosciences, USA). Cells were incubated in the dark for 20 minutes at 4°C, then washed twice in PBS containing 2% FBS with centrifugation at 300 g for 5 minutes at 5°C. All sample data were acquired on a FACSVerse (BD) and analyses were performed using FlowJo software (v. 10.0.8r1) (Treestar, Inc., OR, USA).
Krarup A.R., Abdel-Mohsen M., Schleimann M.H., Vibholm L., Engen P.A., Dige A., Wittig B., Schmidt M., Green S.J., Naqib A., Keshavarzian A., Deng X., Olesen R., Petersen A.M., Benfield T., Østergaard L., Rasmussen T.A., Agnholt J., Nyengaard J.R., Landay A., Søgaard O.S., Pillai S.K., Tolstrup M, & Denton P.W. (2017). The TLR9 agonist MGN1703 triggers a potent type I interferon response in the sigmoid colon. Mucosal immunology, 11(2), 449-461.
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2

Flow Cytometric Analysis of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For flow cytometric analyses, Fc-receptors were blocked with Human Trustain FcX blocking buffer (Biolegend, USA) for 5 minutes at room temperature, and cells were then stained with CD4 PE-Cy7 (SK3); CD8 PerCP-Cy5.5 (RPA-T8); CCR7 FITC (G043H7); CD45RA BV421 (HI100); HLA-DR PE (L243); CD38 BV605 (HIT2) (all from Biolegend, USA). Antibodies were mixed in BD HorizonTM Brilliant Stain Buffer (BD Biosciences, USA). Cells were incubated in the dark for 20 minutes at 4°C, then washed twice in PBS containing 2% FBS with centrifugation at 300 g for 5 minutes at 5°C. All sample data were acquired on a FACSVerse (BD) and analyses were performed using FlowJo software (v. 10.0.8r1) (Treestar, Inc., OR, USA).
Krarup A.R., Abdel-Mohsen M., Schleimann M.H., Vibholm L., Engen P.A., Dige A., Wittig B., Schmidt M., Green S.J., Naqib A., Keshavarzian A., Deng X., Olesen R., Petersen A.M., Benfield T., Østergaard L., Rasmussen T.A., Agnholt J., Nyengaard J.R., Landay A., Søgaard O.S., Pillai S.K., Tolstrup M, & Denton P.W. (2017). The TLR9 agonist MGN1703 triggers a potent type I interferon response in the sigmoid colon. Mucosal immunology, 11(2), 449-461.
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