Pvan software

Manufactured by Millar

Sourced in United States

PVAN software is a data analysis tool developed by Millar. It is designed to process and analyze data from various laboratory equipment and sensors. The software provides users with the ability to visualize, interpret, and manage their experimental data.

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Lab products found in correlation

Spr 839, by Millar (10 mentions) Mpvs 400 system, by Millar (5 mentions) Vevo 770, by Fujifilm (2 mentions) Sodium pentobarbital, by Vortech Pharmaceuticals (2 mentions) Chart 5, by ADInstruments (2 mentions) Mpvs 300, by Millar (2 mentions) Spr 835, by Millar (1 mentions) Vevolab software, by Fujifilm (1 mentions) Avertin, by Merck Group (1 mentions) Automated syringe pump, by B. Braun (1 mentions) Powerlab 8 30, by ADInstruments (1 mentions) Powerlab, by ADInstruments (1 mentions) Labchart, by ADInstruments (1 mentions) Powerlab data acquisition system, by ADInstruments (1 mentions) Dedicated software, by ADInstruments (1 mentions) 1.4f micromanometer catheter, by Millar (1 mentions) Spr 838, by Millar (1 mentions) Catheter system, by Millar (1 mentions) 1.4 f mikro tip catheter, by Millar (1 mentions)

Spelling variants of this product

PVAN data analysis software (6 citations) PVAN data analysis software version 3.3 (3 citations)

27 protocols using pvan software

Comprehensive Cardiac Function Assessment

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Cardiac function was assessed as described previously [20 (link)]. Briefly, mice were anesthetized with isoflurane inhalation (5% for induction, 2% for maintenance) and anticoagulated with heparin (Elkins-Sinn, Cherry Hill, NJ; 1,000 units/kg, IP). Animals were laid supine on a heating blanket and core body temperature was maintained at 37 ± 0.5°C. A pressure-volume microcatheter (Millar Instrument, Houston, TX; 1.0 F) was inserted into the left ventricle (LV) through the right common carotid artery. The pressure-volume loop and heart rate were recorded using the MPVS-400 system with the aid of PVAN software (Millar Instruments, Houston, TX). Heart rate, LV developed pressure, end-systolic and end-diastolic volume, ejection fraction, and cardiac output was analyzed.

Li X., Zhai Y., Yao Q., The E., Ao L., Fullerton D.A., Yu K.J, & Meng X. (2023). Up-regulation of Myocardial Klotho Expression to Promote Cardiac Functional Recovery in Old Mice following Endotoxemia. Research Square.

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Cardiac Function Assessment via Echocardiography

Cited 1 time

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Upon anesthetization, echocardiography was performed with a 30-MHz high-frequency scanhead (VisualSonics Vevo770, VisualSonics) as described previously.⁴³ (link) To monitor LV catheterization, a catheter manometer (Millar 1.4F, SPR 835, Millar Instruments) was inserted via the right carotid artery into the left ventricle. After stabilization, data were continuously recorded. Cardiac function parameters were calculated with the PVAN software (Millar Instruments, Houston, TX, USA) as described.⁴³ (link)

Li H., Dai B., Fan J., Chen C., Nie X., Yin Z., Zhao Y., Zhang X, & Wang D.W. (2019). The Different Roles of miRNA-92a-2-5p and let-7b-5p in Mitochondrial Translation in db/db Mice. Molecular Therapy. Nucleic Acids, 17, 424-435.

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Cardiac Catheterization in Hypoxic Mice

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After a 4-week exposure to hypoxia, the mice were subjected to cardiac catheterisation and haemodynamic analysis, as previously described^{65 (link)}. Briefly, each mouse was anaesthetised via inhaled isoflurane, and the chest was opened via a median sternotomy. A 1.4-F Millar microtip catheter transducer (SPR-839, Millar Instruments, Houston, TX) was inserted to the left ventricular cavity to allow continuous recording of pressure-volume signals. PVAN software (Millar Instruments) was used to analyse the pressure–volume loops according to the manufacturer’s instructions.

Jiang Y.H., Zhu Y., Chen S., Wang H.L., Zhou Y., Tang F.Q., Jian Z, & Xiao Y.B. (2019). Re-enforcing hypoxia-induced polyploid cardiomyocytes enter cytokinesis through activation of β-catenin. Scientific Reports, 9, 17865.

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Comprehensive Cardiac Evaluation Protocol

Cited 1 time

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A 12-lead ECG assay was performed using a commercial system from AD Instruments according to our previously published protocol.72 (link), 73 (link) The Q wave amplitude was determined using the lead I tracing. Other ECG parameters were analyzed using the lead II tracing. The QTc interval was determined by correcting the QT interval with the heart rate, as described by Mitchell et al.⁷⁴ (link) The cardiomyopathy index was calculated by dividing the QT interval by the PQ segment.⁷⁵ Left ventricular hemodynamics was evaluated using a Millar ultra-miniature pressure-volume (PV) catheter SPR 839. The catheter was placed in the left ventricle using a closed chest approach as we previously described.72 (link), 73 (link) The resulting PV loops were analyzed with PVAN software (Millar Instruments). The relaxation constant of the left ventricle was determined using the method of Weiss et al.⁷⁶ (link) Detailed protocols for ECG and hemodynamic assays are available at the Parent Project Muscular Dystrophy standard operating protocol website (http://www.parentprojectmd.org/site/PageServer?pagename=Advance_researchers_sops).⁷⁷ (link)

Hakim C.H., Wasala N.B., Pan X., Kodippili K., Yue Y., Zhang K., Yao G., Haffner B., Duan S.X., Ramos J., Schneider J.S., Yang N.N., Chamberlain J.S, & Duan D. (2017). A Five-Repeat Micro-Dystrophin Gene Ameliorated Dystrophic Phenotype in the Severe DBA/2J-mdx Model of Duchenne Muscular Dystrophy. Molecular Therapy. Methods & Clinical Development, 6, 216-230.

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Cardiac Function Evaluation in LPS-Treated Mice

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We assessed cardiac function at 6 h after LPS treatment as described previously [21 (link),22 (link)]. Briefly, mice were anesthetized with pentobarbital sodium (Vortech Pharmaceuticals, Dearborn, MI, USA; 50 mg/kg, intraperitoneal (ip)) and anticoagulated with heparin (Elkins-Sinn, Cherry Hill, NJ, USA; 1,000 units/kg, ip). Animals were laid supine on a heating blanket and core body temperature was maintained at 37°C ± 0.5°C. A microcatheter (Millar Instruments, Houston, TX, USA; 1 F) was inserted into the left ventricle (LV) through the right common carotid artery. Pressure-volume loop was recorded using the MPVS-400 system with the aid of PVAN software (Millar Instruments). Heart rates, LV pressure, LV volume, and related function parameters were analyzed: 10 μL of 30% saline was injected into the inferior vena cava for actual ventricle volume calculation.

Slimani H., Zhai Y., Yousif N.G., Ao L., Zeng Q., Fullerton D.A, & Meng X. (2014). Enhanced monocyte chemoattractant protein-1 production in aging mice exaggerates cardiac depression during endotoxemia. Critical Care, 18(5), 527.

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LV Hemodynamic Analysis in Septic Mice

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Left ventricle (LV) hemodynamics was analyzed at 6 hours after LPS treatment as described previously [17 (link)]. Briefly, mice were anesthetized with pentobarbital sodium (50 mg/kg, ip; Vortech Pharmaceuticals, Dearborn, Michigan, USA) and anticoagulated using heparin (1,000 units/kg, ip; Elkins-Sinn, Cherry Hill, New Jersey, USA). Animals were laid supine on a heating pad and the core body temperature was kept at 37±0.5°C. A microcatheter (1F; Millar Instruments, Houston, Texas, USA) was introduced into the LV through the right common carotid artery. LV pressure-volume loops were recorded using the MPVS-400 system. LV pressure, LV volume, heart rates, and related function parameters were analyzed with the aid of PVAN software (Millar Instruments, Houston, Texas, USA).

Li J., Zhai Y., Ao L., Hui H., Fullerton D.A., Dinarello C.A, & Meng X. (2017). Interleukin-37 suppresses the inflammatory response to protect cardiac function in old endotoxemic mice. Cytokine, 95, 55-63.

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Cardiac Function Evaluation in Mice

Cited 1 time

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Cardiac function was assessed as described previously [40 (link), 41 (link)]. Briefly, mice were anesthetized with isoflurane inhalation (2% isoflurane mixed with pure oxygen), and anticoagulated with heparin (Elkins-Sinn, Cherry Hill, NJ; 1,000 units/kg, ip). Animals were laid supine on a heating blanket and core body temperature was maintained at 37 ± 0.5°C. A pressure-volume microcatheter (Millar Instruments, Houston, TX; 1 F) was inserted into the LV through the right common carotid artery. Pressure-volume loop was recorded using the MPVS-400 system with the aid of PVAN software (Millar Instruments, Houston, TX). Heart rate, LV developed pressure, ejection fraction and cardiac output were analyzed.

Hui H., Zhai Y., Ao L., Cleveland JC J.r., Liu H., Fullerton D.A, & Meng X. (2017). Klotho suppresses the inflammatory responses and ameliorates cardiac dysfunction in aging endotoxemic mice. Oncotarget, 8(9), 15663-15676.

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Cardiac Function Measurement Protocol

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Cardiac function data were analyzed with PVAN software (Millar Instruments, Houston, TX, USA). All cardiac function parameters were averaged from 8 to 12 cardiac cycles at each time point. End systolic pressure (P_es) was directly measured. Cardiac output (CO), stroke work (SW) and stroke volume (SV) were calculated. VR was calculated as using the MAP divided by the CO (VR=MAP/CO). Oxygen delivery (DO₂) was calculated as the product of the total Hb by the O₂ carrying capacity of saturated Hb (1.34 mLO₂/g_Hb) by the arterial blood O₂ saturation and CO (DO₂=[RBCHb × 1.34 × SA] × CO)46 (link).

Díaz-Trelles R., Scimia M.C., Bushway P., Tran D., Monosov A., Monosov E., Peterson K., Rentschler S., Cabrales P., Ruiz-Lozano P, & Mercola M. (2016). Notch-independent RBPJ controls angiogenesis in the adult heart. Nature Communications, 7, 12088.

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Assessing Mouse Cardiac Hemodynamics

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Mouse hemodynamics were assessed as previously described [47 (link)]. After anaesthetization, left ventricular (LV) catheterization was performed on mice before sacrifice. The portion of the right carotid artery next to the trachea was isolated from the surrounding tissue and nerves. A 1.0 Fr Millar Mikrotip Catheter Transducer (Millar 1.4F, SPR 835, Millar Instruments, Houston, TX) connected to a pressure transducer (Millar Instruments, Houston, TX) was inserted through the right carotid artery into the LV cavity. Hemodynamic parameters were recorded and analyzed with PowerLab/8sp and LabChart 7.2.1 software. PVAN software (Millar Instruments, Houston, TX) was used to perform the cardiac pressure–volume analysis. The peak instantaneous rate of the LV pressure increase and decline (dp/dt max, dp/dt min) were measured. All data were averages of at least five measurements, each measurement concluded at least ten successive loops.

Dai B., Li H., Fan J., Zhao Y., Yin Z., Nie X., Wang D.W, & Chen C. (2018). MiR-21 protected against diabetic cardiomyopathy induced diastolic dysfunction by targeting gelsolin. Cardiovascular Diabetology, 17, 123.

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ECG and Hemodynamic Measurements in Duchenne Models

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Twelve‐lead ECG was performed using a commercial system from AD Instruments (Colorado Springs, CO, USA). Results were evaluated using our previously published standard operating protocol in “Cardiac protocols for Duchenne animal models” (http://www.parentprojectmd.org/site/PageServer?pagename=Advance_researchers_sops).³² All ECG parameters but Q wave amplitude were analyzed using the lead II tracing. Q wave amplitude was determined using the lead I tracing. QTc interval was calculated by correcting the QT interval with the heart rate as described by Mitchell et al.³³ The cardiomyopathy index was calculated by dividing the QT interval by the PQ segment.³⁴ Left ventricular hemodynamics was performed using our previously described closed chest approach with the Millar catheter, which can also be found in the “Cardiac protocols for Duchenne animal models”.³² The resulting pressure‐volume loops were analyzed using the PVAN software (Millar Instruments, Houston, TX, USA). Cardiac relaxation time constant (Tau) was calculated according to Weiss et al.³⁵ Body surface area was calculated as described by Cheung et al.³⁶

Morales E.D., Yue Y., Watkins T.B., Han J., Pan X., Gibson A.M., Hu B., Brito‐Estrada O., Yao G., Makarewich C.A., Babu G.J, & Duan D. (2023). Dwarf Open Reading Frame (DWORF) Gene Therapy Ameliorated Duchenne Muscular Dystrophy Cardiomyopathy in Aged mdx Mice. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 12(3), e027480.

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