Log In Sign up
The largest database of trusted experimental protocols

Shrnas in the plko 1 puro vector for tfam

Manufactured by Merck Group
Visit Supplier

ShRNAs in the pLKO.1-puro vector for TFAM is a laboratory product that allows for the knockdown of the TFAM gene. The pLKO.1-puro vector contains a puromycin resistance cassette and expresses short hairpin RNA (shRNA) targeting the TFAM gene.

Automatically generated - may contain errors

Lab products found in correlation

Trcn0000016095, by Merck Group (2 mentions) Trcn0000016093, by Merck Group (2 mentions) Shrnas in the pgipz vector for tsc1, by Horizon Discovery (2 mentions)

Spelling variants of this product

PLKO.1-puro (99 citations) PLKO.1 (97 citations) PLKO.1 vector (90 citations) PLKO.1-puro vector (73 citations) PLKO vector (19 citations) ShRNA vectors (13 citations) PLKO.1-puro shRNA vector (3 citations) PLKO.1-puro-Tfam (2 citations) PLKO-shRNA vectors (2 citations) PLKO shRNAs (2 citations)

2 protocols using shrnas in the plko 1 puro vector for tfam

1

Lentiviral RNAi Knockdown Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lentiviral RNAi was performed as described2 (link). The shRNAs in the pLKO.1-puro vector for TFAM (sh1: TRCN0000016095; sh2: TRCN0000016093) and PHB2 (sh1: TRCN0000060920; sh2: TRCN0000060921) were obtained from Sigma-Aldrich. The shRNAs in the pGIPZ vector for TSC1 (sh1: V2LHS_92624) and TSC2 (sh1: V2LHS_233694; sh2: V2LHS_93624) were obtained from GE Dharmacon. Sequences of shRNAs are in Supplementary Table 7.
Liu X., Zhang Y., Ni M., Cao H., Signer R.A., Li D., Li M., Gu Z., Hu Z., Dickerson K.E., Weinberg S.E., Chandel N.S., DeBerardinis R.J., Zhou F., Shao Z, & Xu J. (2017). Regulation of Mitochondrial Biogenesis in Erythropoiesis by mTORC1-Mediated Protein Translation. Nature cell biology, 19(6), 626-638.
+ Open protocol
+ Expand
2

Lentiviral RNAi Knockdown Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lentiviral RNAi was performed as described2 (link). The shRNAs in the pLKO.1-puro vector for TFAM (sh1: TRCN0000016095; sh2: TRCN0000016093) and PHB2 (sh1: TRCN0000060920; sh2: TRCN0000060921) were obtained from Sigma-Aldrich. The shRNAs in the pGIPZ vector for TSC1 (sh1: V2LHS_92624) and TSC2 (sh1: V2LHS_233694; sh2: V2LHS_93624) were obtained from GE Dharmacon. Sequences of shRNAs are in Supplementary Table 7.
Liu X., Zhang Y., Ni M., Cao H., Signer R.A., Li D., Li M., Gu Z., Hu Z., Dickerson K.E., Weinberg S.E., Chandel N.S., DeBerardinis R.J., Zhou F., Shao Z, & Xu J. (2017). Regulation of Mitochondrial Biogenesis in Erythropoiesis by mTORC1-Mediated Protein Translation. Nature cell biology, 19(6), 626-638.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!