The human GBM cell lines LN229, A172, LN229, U118 were purchased from the American Type Culture Collection (ATCC). The human GBM cell lines U251 and T98G were obtained from RIKEN bioresource center (Tsukuba, Japan). To maintain authenticity of the cell lines, frozen stocks were prepared from initial stocks, and every 3 months, a new frozen stock was used for the experiments. For each experiment, GBM cells were sustained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. Sodium pyruvate, nonessential amino acids, L-glutamine, and a vitamin solution. NHAs were purchased from Lonza (Walkersville, MD) and maintained following the manufacturer’s instructions.
ShTRIM14, shCtrl, shZEB2, pLVGFP- TRIM14 and pLVGFP- TRIM14 vector were purchased from GenePharma (Shanghai, China). All the plasmids were transfected into cells grown in DMEM culture media using Lipofectamine 3000 Transfection Reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions.
ShTRIM14, shCtrl, shZEB2, pLVGFP- TRIM14 and pLVGFP- TRIM14 vector were purchased from GenePharma (Shanghai, China). All the plasmids were transfected into cells grown in DMEM culture media using Lipofectamine 3000 Transfection Reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions.