Immunohistochemical or immunocytochemical staining for 7 μm wax sections of ONHs or cultured ONH astrocytes were performed as described previously (Ju et al., 2008 (link)). Five sections per wax block from each group (n = 4 ONHs/group) were used for immunohistochemical analysis. The primary antibodies included mouse monoclonal anti-glial fibrillary acidic protein (GFAP) antibody (1:300; Sigma), guinea pig polyclonal anti-GFAP antibody (1:500; Advanced ImmunoChemical. Long Beach, CA), mouse monoclonal anti-neurofilament antibody (1:500; Sigma), mouse monoclonal anti-NR1 antibody (1:1000; BD Pharmingen, San Diego, CA), rabbit monoclonal anti-NR2A antibody (1:100; Millipore, Billerica, MA), rabbit polyclonal anti-NR2B antibody (1:5000; Millipore), and mouse monoclonal anti-DRP1 antibody (1:1000; BD Transduction Laboratories, San Diego, CA). The images were acquired with a FluoView1000 confocal microscope (Olympus, Tokyo, Japan).
Rabbit monoclonal anti nr2a antibody
Manufactured by Merck Group
The Rabbit monoclonal anti-NR2A antibody is a laboratory research tool used for the detection and analysis of the NR2A subunit of the N-methyl-D-aspartate (NMDA) receptor in various biological samples. It is a purified immunoglobulin G (IgG) that is specifically directed against the NR2A protein.
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Lab products found in correlation
Mouse monoclonal anti glial fibrillary acidic protein antibody, by Merck Group (2 mentions)
Mouse monoclonal anti neurofilament antibody, by Merck Group (2 mentions)
Mouse monoclonal anti nr1 antibody, by BD (2 mentions)
Rabbit polyclonal anti nr2b antibody, by Merck Group (2 mentions)
Mouse monoclonal anti drp1 antibody, by BD (2 mentions)
Fluoview 1000 confocal microscope, by Olympus (2 mentions)
2 protocols using rabbit monoclonal anti nr2a antibody
1
Immunostaining of Optic Nerve Head Cells
2
Immunohistochemical Analysis of ONH Astrocytes
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Immunohistochemical or immunocytochemical staining for 7 µm wax sections of ONHs or cultured ONH astrocytes were performed as described previously (Ju et al., 2008 (link)). Five sections per wax block from each group (n = 4 ONHs/group) were used for immunohistochemical analysis. The primary antibodies included mouse monoclonal anti-glial fibrillary acidic protein (GFAP) antibody (1:300; Sigma), guinea pig polyclonal anti-GFAP antibody (1:500; Advanced ImmunoChemical. Long Beach, CA), mouse monoclonal antineurofilament antibody (1:500; Sigma), mouse monoclonal anti-NR1 antibody (1:1,000; BD Pharmingen, San Diego, CA), rabbit monoclonal anti-NR2A antibody (1:100; Millipore, Billerica, MA), rabbit polyclonal anti-NR2B antibody (1:5,000; Millipore), and mouse monoclonal anti-DRP1 antibody (1:1,000; BD Transduction Laboratories, San Diego, CA). The images were acquired with a FluoView1000 confocal microscope (Olympus, Tokyo, Japan).
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