All stains were performed in 96 well u-bottom plates in a volume of 100 μl. 1×105–1×106 cells were plated and blocked with Fc receptor block (BD, Biosciences, San Jose, CA) for 15 min at 4°C and subsequently incubated with antibodies for 1 h at 4°C. Cells were then washed and fixed in 5% formalin for 15 min at room temperature. Flow cytometry was acquired using a FACSCanto RUO system (BD Biosciences). Data analysis was performed using FlowJo (Treestar, Ashland OR). Antibodies included: Siglec F PE (BD), Ly6G APC, Ly6G FITC, CD11b PE-Cy7, CD11c PB, CD71 PerCP Cy5.5, CD206 BV605, ICAM1 FITC, CD45 APC-Cy7, MHCII BV510, CD103 APC (Biolegend).
Cd11c pb
Manufactured by BioLegend
Sourced in United States
The CD11c PB is a lab equipment product that detects the CD11c surface antigen. CD11c is a type I transmembrane glycoprotein that functions as an integrin alpha subunit. It is expressed on the surface of dendritic cells, monocytes, and macrophages, and plays a role in cell adhesion and phagocytosis.
Automatically generated - may contain errors
Lab products found in correlation
Cd11b pe cy7, by BioLegend (3 mentions)
Cd3 apc, by BioLegend (2 mentions)
Siglec f apc cy7, by BioLegend (2 mentions)
Gr 1 pe, by BioLegend (2 mentions)
F4 80 apc, by BioLegend (2 mentions)
Facsdiva software, by BD (2 mentions)
Fc receptor block, by BD (1 mentions)
Ly6g fitc, by BD (1 mentions)
Siglecf pe, by BD (1 mentions)
Cd71 percp cy5, by BioLegend (1 mentions)
Cd206 bv605, by BioLegend (1 mentions)
Icam1 fitc, by BioLegend (1 mentions)
Mhcii bv510, by BioLegend (1 mentions)
Cd103 apc, by BioLegend (1 mentions)
Cd45 apc cy7, by BioLegend (1 mentions)
Ly6g apc, by BD (1 mentions)
Cd203c apc, by Sony (1 mentions)
Hla dr pb, by Sony (1 mentions)
Cd141 apc, by Miltenyi Biotec (1 mentions)
Cd45 po, by Thermo Fisher Scientific (1 mentions)
4 protocols using cd11c pb
1
Multiparameter Flow Cytometry Profiling
2
Immunophenotyping of Leukocyte Subsets
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Leucocytes subsets were identified using an antibody panel containing CD45‐PO (Life Technologies) for lymphocytes; CD123‐PerCPCy5.5 (BD Pharmingen), CD203c APC (Sony), HLA‐DR‐PB (Sony), and CD41‐PE‐Cy7 for basophils; and CD45‐PO (Life Technologies) and CD14‐APC‐H7 (BD Pharmingen) for monocytes. To distinguish between the three different subsets of dendritic cells (DCs), an antibody panel containing HLA‐DR‐PE‐CY7 (BioLegend), CD11c‐PB (BioLegend), and CD123 PerCP Cy5.5 (BD Pharmingen) was used for plasmacytoid dendritic cells (pDCs). For two subsets of myeloid dendritic cells (mDCs), CD14‐V500 (BD), HLA‐DR‐PE‐CY7 (BioLegend), CD1c‐APC‐Cy7 (BioLegend), and CD141‐APC (Miltenyi) were used. Leucocyte subset quantities were depicted as the percentage of cells within the total leucocyte measures/numbers.
3
Multiparameter Flow Cytometry Profiling
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Isolation of lung cells and splenocytes was performed as described elsewhere [8 (link)]. Cell phenotyping was performed on LSRII flow cytometer using FACS Diva software (BD Bioscience, Heidelberg, Germany) and Flowjo software 9.6.6 (Tree Star Inc, San Carlos, CA, USA) with the following anti-mouse monoclonal antibodies: CD45 Peridinin-Chlorophyll-protein (PerCP), CD11b Allophycocyanin-cyanine dye 7 (APC-Cy7), NK1.1 phycoerythrin (PE), CD19 Fluorescein (FITC), CD3 APC, CD4 Alexa Fluor 700 (A700), CD8 Pacific-Blue (PB), Gr1PE, CD11bPE-Cy7, F480 APC, Siglec F APC-Cy7, CD11 c PB (Biolegend, San Diego, CA, USA).
4
Multiparametric Flow Cytometry for Immune Cell Profiling
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Lung cells and splenocytes were isolated as described elsewhere (Engel et al., 2015) and stained with the following anti-mouse monoclonal antibodies for 20 min at 4 • C in the dark: CD45 PerCP, CD11b APC-Cy7, NK1.1 PE, CD19 FITC, CD3 APC, CD4 A700, CD8 PB, Gr1 PE, CD11b PE-Cy7, F4/80 APC, Siglec F APC-Cy7, CD11c PB (Biolegend, San Diego, USA or BD Bioscience, Heidelberg, Germany). Cell phenotyping was performed on LSRII flow cytometer using FACS Diva software (BD Bioscience, Heidelberg, Germany) and analyzed using FlowJo software (Tree Star Inc). Leukocytes were analyzed as follows: Neutrophils in spleen and lung (CD45+/CD11b high /Gr1 high ), Lymphocytes in spleen and lung (B cells: CD45+/CD11b-/CD19+; T cells: CD45+/CD11b-/ CD3+; NK cells: CD45+/NK1.1+/CD3-; NKT cells: CD45+/NK1.1+/ CD3+), alveolar macrophages (CD45+/Gr1-/SiglecF+/CD11c+), macrophages in spleen (CD45+/Gr1-/CD11b+/CD11c-).
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