A 4T1 tumor model was built by injecting 100 μL of 4T1 cell suspension (1 × 106 cells) into the right armpit of female Balb/c mice. To examine the behavior of the RhB-loaded SA-Gd2O3@MSN hybrid systems in acidic pH environments, the mice were randomly assigned to three groups and treated with free RhB, RhB-loaded MSNs (NH2-Gd2O3@MSN-RhB), and SA-Gd2O3@MSN-RhB at a dose of 200 mg/kg RhB via tail vein injection. The fluorescence images (FI) were captured at 1, 6, 12, 24, and 48 h after injection using the IVIS Lumina Ⅲ imaging system (PerkinElmer, Waltham, MA, USA). The 4T1 tumor-bearing mice were injected with 100 μL of SA-Gd2O3@MSN NPs (1 mg of Gd3+ per kg) via the tail vein for in vivo MR imaging. Subsequently, the MR images were captured at 0, 1, and 24 h after injection using a 3.0T clinical MRI scanner (Philips).
Ivis lumina 3 imaging system
Manufactured by PerkinElmer
Sourced in United States
The IVIS Lumina III is a high-performance in vivo imaging system designed for preclinical research. The system utilizes sensitive charge-coupled device (CCD) cameras to capture bioluminescent and fluorescent signals from small animal models, enabling non-invasive visualization and quantification of biological processes within living subjects.
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Lab products found in correlation
Living image software, by PerkinElmer (4 mentions)
D luciferin, by GoldBio (3 mentions)
D luciferin sodium salt, by GoldBio (2 mentions)
Living image software version 4, by PerkinElmer (2 mentions)
Mitosox red probe, by Yeasen (1 mentions)
Balb c nude mice, by Charles River Laboratories (1 mentions)
Living image 4, by PerkinElmer (1 mentions)
Ivis spectrum, by PerkinElmer (1 mentions)
Luminol sodium salt, by GoldBio (1 mentions)
Lucigenin, by Tokyo Chemical Industry (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Igor image analysis fx software, by Wavemetrics (1 mentions)
Living image, by PerkinElmer (1 mentions)
Living image analysis software, by PerkinElmer (1 mentions)
Facsaria 3, by BD (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
Cd90.1 microbeads, by Miltenyi Biotec (1 mentions)
D luciferin, by PerkinElmer (1 mentions)
D luciferin, by Yeasen (1 mentions)
Luciferin, by Promega (1 mentions)
32 protocols using ivis lumina 3 imaging system
1
Tumor Imaging with Hybrid Nanoparticles
2
Oxidative Stress Model in BALB/c Mice
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BALB/c nude mice were purchased from Vital River Laboratories (Beijing, China), weighting about 20 g were chosen for the establishment of an oxidative stress model, LPS (0.02 ml, 1 mg/ml) daily subgingival injection administration. A total of 3 days later, MitoQ@PssL NPs were injected in situ with a dosage of 200 μl per site. A total of 24 h later, the MitoSOXTM Red probe (Yeasen, China) was injected to detect the remaining mtROS. The results of whole-body fluorescence imaging were used an IVIS® Lumina Ⅲ imaging system (PerkinElmer, United States). ImageJ software was used to measure and quantify the fluorescence signal intensity.
3
Ex vivo Fluorescence Imaging of NPs
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Ex vivo optical imaging was performed on the excised brain and spinal cord after fluorescent NP administration. Fluorescence images were acquired with an IVIS Lumina III imaging system (PerkinElmer, Waltham, MA, USA). The following acquisition parameters were used: excitation filter range from 680 to 740 nm, emission filter (790 nm, exposure time 2 s), binning factor 4, and f/Stop 2. Spectral unmixing, image processing, and analysis were performed using Living Image 4.3.1 software (PerkinElmer).
4
In vivo Bioluminescence Tumor Imaging
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On days one, three and eight, following the inoculation of bioluminescent tumor cells, the animals underwent in vivo imaging using the IVIS Lumina III imaging system (PerkinElmer, Waltham, MA, USA). The transfected 4T1 tumor cells express firefly luciferase which produces bioluminescence following the addition of its substrate (luciferin). In consideration of the imaging, a 15 mg/ml solution of D−luciferin sodium salt (Gold Biotechnology, St. Louis, MO, USA) in DPBS was administered i.p. in a 150 mg/kg dose. Animals were anesthetized using ketamine-xylazine three minutes following the administration of D-luciferin, and were imaged ten minutes postinjection using the following settings: auto acquisition, F/stop = 1 and Binning = 4. Data were analyzed using the Living Image software (PerkinElmer). Regions of interest (ROI) were drawn around the luminescent tumor signal automatically using identical signal thresholds. Total radiance, a calibrated unit of the luminescence (total photon flux per second), was calculated in each ROI and used for further statistical analysis.
5
Bioluminescent Imaging of Postsurgical Infection
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Postsurgical infection was confirmed by direct BLI of bacteria using the IVIS Lumina III imaging system (PerkinElmer, Hopkinton, MA). Imaging (large binning and a 5 min exposure) was performed immediately on anesthetized mice (2% isoflurane) before surgery and then on postoperative days 3, 7, 14, and 21. Signal was quantified using the Living Image software within a region of interest (ROI) of 0.5 × 0.75 cm2 centered over the surgical knee and measured as maximum radiance (photons/second/cm2/steradian). The limit of detection was 2.5 × 103 photons/s/cm2/sr. After an infection was established, the right hind limb was cleared of hair, and a bioluminescent image was acquired (IVIS Spectrum; Perkin Elmer). An elliptical ROI of 0.74 × 0.98 cm was used to measure persistent infection.
6
Imaging Inflammatory Reactions in Joints
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Inflammatory reaction of the joints was examined by in vivo bioluminescence imaging. We measured neutrophil myeloperoxidase (MPO) activity with luminol (5-amino-2,3-dihydro-1,4-phthalazine-dione) and macrophage NADPH oxidase (Phox) activity with lucigenin (bis-N-methylacridinium nitrate) in order to detect reactive oxygen species [48 (link)]. Imaging of the animals was performed 10 min after the i.p. injection of luminol sodium salt (150 mg/kg; Gold Biotechnology, Olivette, MO, USA) dissolved in phosphate buffered saline (PBS; 30 mg/mL) and lucigenin (25 mg/kg; Tokyo Chemical Industry Co., Ltd., Tokyo, Japan) dissolved in saline (2.5 mg/mL) using the IVIS Lumina III imaging system (PerkinElmer, Waltham, MA, USA). Acquisition times were 120 s for luminol-derived bioluminescence and 180 s for lucigenin-derived bioluminescence (Binning = 8, F/Stop = 1). Identical regions of interest (ROIs) were applied on the hind paws and ankle joints, and luminescence was expressed as the total radiance (total photon flux/s) within the ROIs [49 (link)]. Animal numbers: Acute Control (n = 24); Acute CFA (n = 21); Chronic Control (n = 9–10); Chronic CFA (n = 10).
7
Preclinical Evaluation of Anti-FGF19 and G1A8
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Mice (6‐8 weeks old NOD SCID and NSG) were s.c. injected with 5 × 106 Hep3B cells in the right flank. Based on similar mean tumor bioluminescence intensities or tumor volumes, mice were divided into groups and received i.p. injection of 10 mg/kg anti‐FGF19 or control Ab. Tumor bioluminescence intensities were measured using an IVIS Lumina III Imaging System (PerkinElmer). Tumor volume was measured with an electronic caliper and calculated using the formula 3.14 × L × W2/6, where L and W are the largest and smallest measured diameters, respectively. All animal experiments were carried out following the National Guidelines for Housing and Care of Laboratory Animals in China and under the approved Institutional Animal Care and Use Committee (IACUC) protocols at the National Institute of Biological Sciences. The study to evaluate antitumor efficacy of HS29 in PDXs in BALB/c nude mice was undertaken under the approved IACUC protocols at Crown Bioscience.
Safety assessment of G1A8 was carried out at JOINN Laboratories following approved IACUC protocols. Healthy cynomolgus monkeys (3‐4 years old) weighing approximately 3 kg were i.v. injected with G1A8. Blood samples were collected at various time points for pharmacokinetic analysis of G1A8. Liver, ileum, and kidney samples were collected at the end of the study for mRNA expression analysis of genes related to bile acid metabolism.
Safety assessment of G1A8 was carried out at JOINN Laboratories following approved IACUC protocols. Healthy cynomolgus monkeys (3‐4 years old) weighing approximately 3 kg were i.v. injected with G1A8. Blood samples were collected at various time points for pharmacokinetic analysis of G1A8. Liver, ileum, and kidney samples were collected at the end of the study for mRNA expression analysis of genes related to bile acid metabolism.
8
Bioluminescence Imaging of Luciferase-Expressing Tumors
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Bioluminescence imaging was performed as previously described34 (link). Briefly, mice bearing tumor with luciferase were injected IP with 80 mg/kg D-luciferin (Gold Biotechnology). After 10 min, bioluminescence signals were recorded with IVIS Lumina III Imaging System (Perkin Elmer) and analyzed with Living Image Software version 4.5 (Perkin Elmer).
9
Bioluminescent Tumor Cell Seeding
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B16F10 and B16F10/effluc cells were seeded at various cell densities in black, clear-bottomed 96-well plates. After 24 h, 3 µl (3 mg/mL) ᴅ-luciferin was added to each well, and effluc activity was measured using an IVIS® Lumina III imaging system (PerkinElmer, Waltham, MA, USA).
10
Evaluating DEX Effects on TAMs
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To evaluate the effect of DEX (Sigma, St. Louis, MO) on both TAMs migration as well as on TAMs modulation of tumor growth, CT26/RM tumor-bearing mice were divided into three groups: CT26/RM, CT26/RM+Raw/effluc, and CT26/RM+Raw/effluc+DEX. Mice received 10 mg/kg DEX immediately via intraperitoneal injection following the transfer of Raw/effluc cells. Daily treatment with DEX was sustained for 3 days. In vivo BLI and FLI were performed at the indicated times.
For in vivo BLI, mice received eitherd -luciferin (for effluc) or coelenterazine h (for Rluc) via either intraperitoneal or intravenous injection. BLI was performed at 10 minutes (effluc) or immediately (Rluc) after the injection of the respective substrate using the IVIS Lumina III imaging system (PerkinElmer). Grayscale photographic images and bioluminescent color images were superimposed using LIVINGIMAGE (version 2.12, PerkinElmer) and IGOR Image Analysis FX software (WaveMetrics, Lake Oswego, OR). BLI signals are expressed in units of photons per cm2 per second per steradian (P/cm2/s/sr). All mice were anesthetized using 1% to 2% isoflurane gas during imaging.
For in vivo BLI, mice received either
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