Antibodies to β-actin and HA tag were purchased from Cell Signaling Technologies. Antibodies against double stranded DNA and Histone-H3 (tri-methyl K9) were purchased from Abcam. β1-integrin and α-tubulin antibodies were purchased from MilliporeSigma. CD63 antibody used for immunoblotting was obtained from System Biosciences International. VAMP3, ARF6, Emerin, APEX1, Annexin-A1, Flotillin, Alix, CD81, TSG-101, SRY, and Histone H-3 antibodies, along with rabbit polyclonal antibody against cGAS, were purchased from Proteintech. Mouse monoclonal antibody against human CD63 (H5C6) used for immunofluorescence was purchased from the Developmental Studies Hybridoma Bank. Antibody against GFP was purchased from Life Technologies. Fluorophore conjugated secondary antibodies (donkey anti-mouse Alexa Fluor Plus 488, donkey anti-rabbit Alexa Fluor Plus 555, donkey anti-mouse Alexa Fluor Plus 680, and donkey anti-rabbit Alexa Fluor Plus 800), rhodamine phalloidin, Alexa Fluor 647-phalloidin, To-Pro-3 iodide, Nuc Green stain, and DRAQ-5 were purchased from Life Technologies. High capacity magnetic sepharose beads were purchased from MilliporeSigma. Unless noted, all other chemicals were purchased from V.W.R.
Annexin a1
Manufactured by Proteintech
Annexin-A1 is a member of the annexin family of calcium-dependent phospholipid-binding proteins. Annexins have the ability to bind to phospholipids in the presence of calcium. Annexin-A1 plays a role in various cellular processes, including regulation of inflammation, apoptosis, and membrane organization.
Automatically generated - may contain errors
Lab products found in correlation
Rhodamine phalloidin, by Thermo Fisher Scientific (2 mentions)
Draq5, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 647 phalloidin, by Thermo Fisher Scientific (2 mentions)
Antibodies to β actin and ha tag, by Cell Signaling Technology (2 mentions)
Antibodies against double stranded dna, by Abcam (2 mentions)
Histone h3 tri methyl k9, by Abcam (2 mentions)
Vamp3, by Proteintech (2 mentions)
Emerin, by Proteintech (2 mentions)
Apex1, by Proteintech (2 mentions)
Mouse monoclonal antibody against human cd63 h5c6, by Developmental Studies Hybridoma Bank (2 mentions)
Alexa fluor plus 488, by Thermo Fisher Scientific (2 mentions)
Alexa fluor plus 555, by Thermo Fisher Scientific (2 mentions)
Alexa fluor plus 680, by Thermo Fisher Scientific (2 mentions)
Alexa fluor plus 800, by Thermo Fisher Scientific (2 mentions)
Nuc green stain, by Thermo Fisher Scientific (2 mentions)
α tubulin, by Merck Group (2 mentions)
Integrin β1, by Merck Group (2 mentions)
High capacity magnetic sepharose beads, by Merck Group (2 mentions)
Tsg101, by Proteintech (2 mentions)
Histone h3, by Proteintech (2 mentions)
3 protocols using annexin a1
1
Antibody Reagents for Extracellular Vesicle Analysis
2
Western Blot Analysis of Virus-Infected Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The infected and mock-infected cells were collected at 36 h pi. Equivalent amounts of cell lysates from each sample were mixed with 5 × sample loading buffer and boiled for 10 min, then separated by 12% SDS-PAGE. The proteins were electrotransferred to 0.45 μm PVDF membranes (Millipore). Membranes were blocked with 5% (w/v) skim milk-TBST at room temperature for 2 h and then incubated overnight at 4 °C with primary antibodies to calnexin (Cell Signaling Technology, Inc.), phospho-p70S6K (Cell Signaling Technology, Inc.), annexin A1 (Protein-Tech, Inc.), and other antibodies purchased from ABclonal Technology. The membranes were washed with TBST and then incubated with horseradish peroxidase (HRP) conjugated goat anti-rabbit IgG (ABclonal, Inc.) or goat anti-mouse IgG (ABclonal, Inc.) at 37 °C for 1 h. The signals were obtained using the clarity-enhanced chemiluminescence (ECL) reagent (Bio-Rad, Hercules, CA).
3
Antibody Reagents for Extracellular Vesicle Analysis
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