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2 protocols using f 12 nutrients

1

Comprehensive Cell Culture Protocol

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Dulbecco’s Modified Eagle Medium (DMEM) (Sigma; cat#D8437), 0.05% trypsin (Thermo Fisher, cat# 25300120), 10% fetal bovine serum (Omega; cat#FB11), 100 μg ml−1 streptomycin and 100 U ml−1 penicillin (Thermo Fisher; cat#15140122), RPMI-1640 Medium (Sigma cat#R8758), Geneticin (Thermo Fisher; cat#11811031), Matrigel (VWR; cat#47743–716), B-27 Supplement, (Thermo Fisher; cat#17504–044), 0.1% Insulin, (Sigma; cat#I6634), 0.1% apo-transferrin, (Sigma; cat#T1428), 0.1% Putrescine (Sigma; cat#P5780). B16 Base Media (pH 7.35): [740 ml DMEM (GIBCO; cat#31053–036), 260 ml F-12 nutrients (GIBCO; cat#21700–075), 14 mM sodium bicarbonate, 11 mM HEPES (Sigma; Cat#H6147) and 1% BSA (Sigma; Cat#A3311) in 1 L of MilliQ water].
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2

Comprehensive Cell Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dulbecco’s Modified Eagle Medium (DMEM) (Sigma; cat#D8437), 0.05% trypsin (Thermo Fisher, cat# 25300120), 10% fetal bovine serum (Omega; cat#FB11), 100 μg ml−1 streptomycin and 100 U ml−1 penicillin (Thermo Fisher; cat#15140122), RPMI-1640 Medium (Sigma cat#R8758), Geneticin (Thermo Fisher; cat#11811031), Matrigel (VWR; cat#47743–716), B-27 Supplement, (Thermo Fisher; cat#17504–044), 0.1% Insulin, (Sigma; cat#I6634), 0.1% apo-transferrin, (Sigma; cat#T1428), 0.1% Putrescine (Sigma; cat#P5780). B16 Base Media (pH 7.35): [740 ml DMEM (GIBCO; cat#31053–036), 260 ml F-12 nutrients (GIBCO; cat#21700–075), 14 mM sodium bicarbonate, 11 mM HEPES (Sigma; Cat#H6147) and 1% BSA (Sigma; Cat#A3311) in 1 L of MilliQ water].
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