Fatty acids were determined by gas mass spectrometry (Trace 1310-ISQ 7000, Thermo Fisher, Waltham, MA, USA). The injection volume was 1 μL, the split ratio was 8:1, the inlet temperature was 250 °C, the ion source temperature was 230 °C, the transmission line temperature was 250 °C, the quadrupole temperature was 150 °C and the carrier gas flow rate was 0.63 mL/min.
Trace 1310 isq 7000
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Trace 1310-ISQ 7000 is a gas chromatography-mass spectrometry (GC-MS) system designed for analytical applications. It combines a Thermo Scientific Trace 1310 gas chromatograph with the Thermo Scientific ISQ 7000 single quadrupole mass spectrometer. The system provides high-performance separation and detection capabilities for a variety of analytical tasks.
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Lab products found in correlation
4 protocols using trace 1310 isq 7000
1
Fatty Acid Analysis by GC-MS
2
Gushi Chicken Fatty Acid Profiling
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To obtain meat quality trait phenotypic data, we analyzed the different fatty acid composition contents of Gushi chicken breast muscles tissues at three developmental stages: 14, 22, and 30 weeks of age. The content of fatty acids in breast muscle tissue was performed by Trace 1310-ISQ 7000 gas chromatography-mass spectrometry (GC–MS) (Thermo Fisher Scientific, Shanghai, China), in accordance with the PRC National Standard “Meat and meat products—determination of fatty acids (GB/T 9695.2–2008).” In plain terms, we assessed the 49 kinds of fatty acids content in Gushi chicken breast muscle tissue and 16 kinds of fatty acid metabolism traits. The fatty acids content was expressed as the percentage of the breast muscle by fresh weight (μg/g).
3
Fecal SCFA Quantification Protocol
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Fecal SCFA levels at the end of the intervention period were measured using a Trace GC Ultra Gas Chromatograph coupled with an ISQ Mass Spectrometer (TRACE 1310-ISQ 7000, Thermo, MA, United States). Final data were normalized based on fecal weight.
4
Volatile Organic Compounds Analysis by GC-MS
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VOCs in samples were analyzed by TRACE1310/ISQ7000 mass selective detector (ThermoFisher) with TG-5MS column (30 m × 0.25 mm × 0.25 µm; ThermoFisher). The injection was set to splitless mode for 5 min at 250 °C at a helium flow rate of 1.0 mL/min. The temperature program used 40 °C for 2 min then heating by 4 °C/min to 160 °C for 4 min and finally, to 250 °C at 15 °C/min which was maintained for 2 min. The temperature of the inlet pipe was set to 250 °C and the temperature of the ion source was set to 230 °C. The MS detector used in positron ionization (EI+) mode of 70 eV and the mass scanning range was 35–450 amu (m/z). Preliminary identification was conducted with mass spectrometry data deposited in the National Institute of Standards and Technology database (NIST 11). Identification was further achieved based on the possible percentages of the three candidate components provided by GC–MS. The relative content of each compound was calculated by the peak area normalization method where the total percentage of the peak area represents the sum of the peak areas of all identified compounds.
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