HFMCD NASH model: 6–8-week-old male C57BL/6N mice were fed with a high-fat methionine-choline-deficient (HFMCD) diet consisting of 60 kcal% fat and 0.1% methionine by weight (A06071302; Research Diets, New Brunswick, NJ, USA). Littermate mice fed with normal chow diet (NCD) were used as the control mice. The mice were divided into three groups: NCD, HFMCD diet alone for 4 weeks, and HFMCD diet with RNase 1 (50 ug/kg). Rnase1 was injected intra-peritoneally, starting every alternate day after 2 weeks of feeding on the HFMCD diet, and continued for the next 2 weeks. All animals were sacrificed after 4 weeks, and serum and liver tissues were collected. All animal procedures were carried out in accordance with the institutional guidelines for animal research at SGPGIMS.
A06071302
Manufactured by Research Diets
Sourced in United States
A06071302 is a laboratory instrument used for the measurement and analysis of various parameters. It is designed to provide accurate and reliable data in a research setting. The core function of this product is to serve as a tool for data collection and analysis, with the specific details of its intended use not included in this factual description.
Automatically generated - may contain errors
Lab products found in correlation
D12492, by Research Diets (4 mentions)
A06071314, by Research Diets (3 mentions)
Cdahfd, by Research Diets (3 mentions)
Td 88137, by Inotiv (2 mentions)
A13012807, by Research Diets (2 mentions)
C57bl 6j mice, by Jackson ImmunoResearch (2 mentions)
Male c57bl 6 mice, by Charles River Laboratories (2 mentions)
C57bl 6j mice, by Japan SLC (2 mentions)
D09100301, by Research Diets (1 mentions)
C57bl 6j, by Charles River Laboratories (1 mentions)
D10042101, by Research Diets (1 mentions)
C57bl 6j mice, by CLEA Japan (1 mentions)
Carbon tetrachloride ccl4, by Merck Group (1 mentions)
Diphtheria toxin from corynebacterium diphtheriae, by Merck Group (1 mentions)
Rosa26idtr, by Jackson ImmunoResearch (1 mentions)
B6 129s1 rnlstm1gvd j mice, by Jackson ImmunoResearch (1 mentions)
Ldlr mice, by Jackson ImmunoResearch (1 mentions)
A02082003by, by Research Diets (1 mentions)
A02082002br, by Research Diets (1 mentions)
Oil red o working solution, by Merck Group (1 mentions)
37 protocols using a06071302
1
HFMCD Diet-Induced NASH Model
2
Mouse Model of NASH Development and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male C57BL/6J mice were obtained from Shanghai SLAC Laboratory and carefully housed under pathogen-free conditions. Mice were fed with a NASH diet containing 40% fat (of which 18% was trans-fat), 22% fructose, and 2% cholesterol (D09100301, Research Diets Inc., New Brunswick, NJ, USA) or a normal diet for 16 or 20 weeks. Mice were fed with a choline-deficient, l -amino acid-defined, high-fat diet with a 0.1% methionine (CDAHFD) diet (A06071302, Research Diets Inc.) or a control diet (A06071314, Research Diets Inc.) for 6 weeks. All animal-related experimental procedures were performed in accordance with the National Institutes of Health guidelines and approved by the Laboratory Animal Ethical Committee of Fudan University. After being fed separately, the mice were sacrificed for analysis. Just before sacrifice, blood was drawn directly from the heart, and the serum was separated and stored at −80°C. Liver tissues were rapidly harvested and fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) or snap-frozen in liquid nitrogen and stored at −80°C for later assessment.
3
Modulating atherosclerosis in Ldlr-deficient mice
4
Dietary Intervention in C57BL/6J Mice
5
Liver Injury and Fibrosis in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal experiments were approved by the Massachusetts General Hospital Institutional Animal Care and Use Committee (IACUC). Animals were maintained on a 12-hour light/dark cycle at 25 °C and fed a standard diet. C57BL/6J mice were purchased from Charles River Laboratories (Wilmington, MA). Pxdn-/- mice were kindly provided by Dr Gautam Bhave from Vanderbilt University Medical Center (Nashville, TN). To induce liver injury by toxin and cause fibrosis and cirrhosis, mice were given 3 times per week of 20% to 40% CCl4 via oral gavage (Sigma-Aldrich, St. Louis, MO) according to our established protocols.69 (link) To create NAFLD animal model, 8-week-old male C57BL/6J mice were purchased from Charles River Laboratories (Wilmington, MA) and housed in a specific pathogen-free environment according to our IACUC protocol. After 1 week of acclimation, mice were fed on either normal chow (Prolab Isopro 3000; Scotts Distributing #8670) or L-amino acid diet with 60 kcal% fat with 0.1% methionine without added choline (CDAHFD); Research Diets A06071302) for 16 weeks. In both models, the well-being of animals was observed daily, and with any sign of distress or pain, mice were euthanized immediately.
6
Choline-Deficient, DDC, and CDAHFD Diets for Liver Disease
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the CDE diet, eight-week-old female mice were housed individually and fed the choline-deficient diet (02960034, MP Biomedicals) and drinking water containing 0.15% DL-ethionine (E5139, Sigma) for 4 weeks. For the DDC diet, 8-week-old male mice were fed the diet containing 0.1% 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) (CLEA Japan) for 4 weeks. For CDAHFD, eight-week-old mice were fed the CDAHFD diet (A06071302, Research Diets) for 12 weeks.
7
Tumor and Fatty Liver Mouse Models
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All protocols for animal experiments were approved by the Ethical Committee on Animal Experiments of Gunma University (18-018), and all animal experiments were conducted under the institutional guidelines. All experiments were performed in compliance with the ARRIVE guidelines. Seven- to nine-week-old Balb/c male mice (CLEA Japan) were used in this study. Tumor transplants were established in 7-week-old male athymic Balb/c nude mice (nu/nu) (CLEA Japan) by subcutaneous injection of a suspension of HCT-116 cells [5 × 106 cells in 150 μL PBS:Matrigel (1:1 v/v)]. Experiments with tumor-bearing mice were performed 2 weeks after the injection of tumor cells. Fatty liver model mice were generated by feeding 6-week-old Balb/c male mice (CLEA Japan) with a choline-deficient, l -amino acid-defined, high-fat diet (CDAHFD, A06071302, Research Diets) consisting of 60 kcal% fat and 0.1% methionine for 2 weeks42 (link).
8
Dietary-induced NASH and gonadectomy models
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animals were purchased from the Jackson Laboratory and bred at UCLA according to approved IACUC protocols with daily monitoring by vivarium personnel. We have previously described both the HMDP and gonadectomy study design in detail.17 (link) Briefly, 8-week-old males and females of ∼98 inbred strains were fed a HF/HS diet (Research Diets-D12266B) for 8 weeks. For the gonadectomy studies, at 6 weeks of age, both sexes of C57BL/6J mice were either gonadectomized or sham-operated under isoflurane anesthesia and placed on a HF/HS diet around 8 weeks of age. For NASH models, 8-week-old C57BL/6J-APOE-Leiden were fed a Western diet (Research Diets-D10042101) for 16 weeks,31 (link) or C57BL/6J mice were fed CDAHFD (Research Diets-A06071302) for 12 weeks.30 (link)
9
Dietary Intervention in C57BL/6J Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C57BL/6J mice were obtained from Clea Japan, Inc. (Tokyo, Japan). A CDAHFD with 0.1% methionine (A06071302; Research Diets, New Brunswick, NJ) was purchased, and its composition is shown in Table 1 . α-Toc (above 99.8% purity) and a T3 mixture (32% α-T3, 5% β-T3, 48% γ-T3, and 15% δ-T) were provided by MITSUBIHSHI CHEMICAL FOODS Co., Ltd. (Tokyo, Japan). Vit E-free stripped corn oil was kindly donated by Tama Biochemical (Tokyo, Japan).
10
Combination therapy for NAFLD
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Both pharmacologic compounds were kindly provided by Bristol-Myers-Squibb. The CCR2/5 antagonist (BMS-687681) was dissolved in sterile water at pH 3 containing 0.5% methylcellulose (400 cps) and 0.1% Tween-80. The CCR2/5 antagonist was administered via oral gavage (PO) at either 45 mg/kg body weight (BW) b.i.d. in single drug treatment or 15 mg/kg BW b.i.d. in combination treatment. PEG-FGF21 variant (BMS-986171) was suspended in a vehicle containing 20 mM Tris(hydroxymethyl)aminomethane and 250 mM sucrose at pH 8.3. PEG-FGF21v was administered by subcutaneous (SC) injection at 0.6 mg/kg BW twice weekly.
Carbon tetrachloride (CCl4) (Merck, Darmstadt, Germany) solved in corn oil was injected once intraperitoneally (IP) at 0.6 mL/kg BW to induced acute liver injury. All mice were sacrificed after 36 h and liver and blood samples were retrieved for analysis.
As a representative NAFLD model, mice were fed a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) (A06071302, Research Diets, New Brunswick, NJ 08901, USA) for up to 12 weeks. Pharmacologic treatment started as single drug or combination therapy after 6 weeks of diet administration. Mice were sacrificed after two or six weeks of treatment for final analysis.
Carbon tetrachloride (CCl4) (Merck, Darmstadt, Germany) solved in corn oil was injected once intraperitoneally (IP) at 0.6 mL/kg BW to induced acute liver injury. All mice were sacrificed after 36 h and liver and blood samples were retrieved for analysis.
As a representative NAFLD model, mice were fed a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) (A06071302, Research Diets, New Brunswick, NJ 08901, USA) for up to 12 weeks. Pharmacologic treatment started as single drug or combination therapy after 6 weeks of diet administration. Mice were sacrificed after two or six weeks of treatment for final analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!