Vega 3 electron microscope

Specific surface area, pore volume and pore size distribution were investigated using Quantachrome Nova 1200e by nitrogen adsorption at 77 K and analyzed by the BET and BJH equations. Prior to analysis, all samples were degassed at room temperature for 48 hours. The samples for transmission electron microscopy (TEM) were obtained by dispersing a small probe in ethanol to form a homogeneous suspension. Then, a suspension drop was coated on a copper mesh covered with carbon for a TEM analysis (FEI TECNAI G2 F20, at an operating voltage of 200 kV). To analyze the samples using high-resolution scanning electron microscopy (SEM), the obtained ground xerogel was deposited on a metal tip and investigated without additional spraying using a Magellan 400 L ultra-high resolution electron microscope. To analyze plasma clot using SEM, the obtained samples were dried under vacuum for 1 hour and investigated without additional spraying using a TESCAN VEGA 3 electron microscope. Optical microscopy was done on a LOMO MIKMED 6 microscope with an X10 lens. Hydrodynamic diameter was measured by the DLS technique on Photocor Compact Z. Spectrophotometrical measurements of enzymatic activity were carried out using an Agilent Cary HP 8454 Diode Array spectrophotometer with TEC.

The crystal phase and crystallinity of the samples were studied by the X-ray diffraction method (Rigaku SmartLab 3 diffractometer of the Engineering center of the Saint-Petersburg State Technological Institute (Technical University)) using Cu-Kα irradiation (λ = 1.54 Å), with samples being scanned along 2θ in the range of 5–80° at a speed of 0.5°/min. For XRD analysis, the samples were dried at 120 °C for 4 h. The particle size and zeta potential of NPs were measured using a Photocor Compact Z. For SEM analysis, the samples were dried in vacuo for 1 h and then examined using a Tescan VEGA 3 electron microscope. The FTIR spectra were obtained by Nicolet iS5 spectrometer. The samples were dried in oven at 60 °C overnight, powdered with agate mortar, and then embedded in mineral oil for measurement.